Today it is generally accepted that the amino acid sequence of a protein determines its three-dimensional structure as well as its function. This means that the amino acid sequence contains much of what we would like to know about a protein, if only we could extract it. The challenge of extracting this information has stimulated many approaches to this “protein folding problem.” This chapter describes some of the most commonly used techniques for extracting some of the information contained in a protein sequence. Most methods discussed here are based on assumptions and approximations that are not always valid. Therefore, the results have to be analyzed carefully and accepted only with caution.
KeywordsTransmembrane Helix Vertical Step Variation Moment Pancreatic Ribonuclease Horizontal Step
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