PCR offers a means of much more quickly and easily modifying DNA fragments to meet specific needs than was previously available. This is because of the ease of PCR itself compared to recombinant DNA techniques, because sequence changes can much more readily be made in oligonucleotide primers chemically than by manipulating DNA fragments with restriction and ligation enzymes, and because PCR products can readily accept such sequence changes as 5’ “add-on” sequences to the primers. Furthermore, the efficiency at which modified product is produced is nearly 100%.
KeywordsAcquire Immune Deficiency Syndrome Sequence Alteration Excess Primer Ligation Enzyme Specific Mutagenesis
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