Abstract
The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E. coli DNA Polymerase I, Klenow fragment (PolI-Kf).2,3 Since Taq DNA Polymerase can withstand repeated exposure to the high temperatures (94°–95°C)1 required for strand separation, the tedium and frequent rebellion resulting from having to add PolI-Kf after each cycle is minimized.
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© 1989 Stockton Press
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Gelfand, D.H. (1989). Taq DNA Polymerase. In: Erlich, H.A. (eds) PCR Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-20235-5_2
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DOI: https://doi.org/10.1007/978-1-349-20235-5_2
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-0-333-48948-2
Online ISBN: 978-1-349-20235-5
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