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PCR Technology pp 225-233 | Cite as

Detection of ras Oncogenes Using PCR

  • Johannes L. Bos

Abstract

Cancer is thought to be mainly due to alterations in the cellular genome that affect the expression or function of genes controlling cell growth and differentiation. Present-day molecular cancer research aims at identifying the alterations responsible for the development of tumors, at characterizing the genes involved and at determining the consequences of the gene alterations for the control of cell growth and differentiation and for the process of carcinogenesis. Five major approaches have been used to identify genes that may place a role in carcinogenesis: 1) characterization of cellular sequences transduced to the genome of acutely transforming retroviruses, 2) transfection of tumor DNA into mouse NIH/3T3 cells and selection for transforming genes, 3) isolation of genes altered by insertion or viral sequences, 4) direct isolation of altered genes from tumor cells with a visible alteration, such as a translocation in their chromosomes, and 5) isolation of genes with sequence similarities to genes discovered by the other approaches. By these procedures, a large number of genes have been identified, but to date only a fraction of these have been implicated in the development of human tumors. Of these, the ras family has been the most thoroughly investigated.

Keywords

Acute Myeloid Leukemia Familial Adenomatous Polyposis Papillary Carcinoma Follicular Adenoma Kras Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Barbacid, M. (1987) Ann. Rev. Biochem. 56:779-827.CrossRefGoogle Scholar
  2. 2.
    Bos, J.L., Verlaan-de Vries, M. Janssen, A.M., Veeneman, G.H., van Boom, J.H., and van der Eb, A.J. (1984) Nucl. Acids Res. 12:9155–9163.CrossRefGoogle Scholar
  3. 3.
    Verlaan-de Vries, M., Bogaard, M.E., van den Elst, H., van Boom, J.H., van der Eb, A.J., and Bos, J.L. (1986) Gene 50:313–320.CrossRefGoogle Scholar
  4. 4.
    Bos, J.L., Fearon, E.R., Hamilton, S.R., Verlaan-de Vries, M., van Boom, J.H., van der Eb, A.J., and Volgelstein, B. (1987) Nature 327:293–297.CrossRefGoogle Scholar
  5. 5.
    Shibata, D., Arnheim, N., and Martin, J. (1988) J. Exp. Med. 167:225–230.CrossRefGoogle Scholar
  6. 6.
    Saiki, R., Sharf, S., Faloona, F., Mullis, K., Horn, G., Ehrlich, H.A., and Arnheim, N. (1985) Science 230:1350–1353.CrossRefGoogle Scholar
  7. 7.
    Winter, E., Yamamoto, F., Almoguera, C., and Perucho, M. (1985) Proc. Natl. Acad. Sci. USA 82:7575–7579.CrossRefGoogle Scholar
  8. 8.
    Almoguera, C., Shibata, D., Forrester, K., Martin, J., Arnheim, N., and Perucho, M. (1988) Cell 53:549–554.CrossRefGoogle Scholar
  9. 9.
    McMahon, G., Davis, E., and Wogan, G.N. (1987) Proc. Natl. Acad. Sci. USA 84:4974–4978.CrossRefGoogle Scholar
  10. 10.
    Collins, S.J. (1988) Mutat. Res. 195:255–271.CrossRefGoogle Scholar
  11. 11.
    Bos, J.L. (1988) Mutat. Res. 195:255–271.CrossRefGoogle Scholar
  12. 12.
    Smit, V.T.H.B.M., Boot, A.J.M., Smits, A.M.M., Fleuren, G.J., Comelisse, CJ., and Bos, J.L. (1988) Nucl. Acids Res. 16:7773–7782.CrossRefGoogle Scholar
  13. 13.
    Qualfe, CJ., Pinkert, CA. Omitz, D.M., Palmiter, R.D., and Brinster, R.L. (1987) Cell 48:1032–1034.Google Scholar
  14. 14.
    Vogelstein, B., Fearon, E.R., Hamilton, S.R., Kern, S.E., Preisinger, A.C., Leppert, M., Nakamura, Y., Whyte, R., Smits, A.M.M., and Bos, J.L. (1988) 319:525-532.Google Scholar
  15. 15.
    Forrester, K., Almoguera, C., Han, K., Grizzle, W.E., and Perucho, M. (1987) Nature 327:298–303.CrossRefGoogle Scholar
  16. 16.
    Rodenhuis, S., van de Wetering, M.L., Mooi, WJ., Evers, S.G., van Zandwijk, N., and Bos, J.L. (1987) N. Engl. J. Med. 317:929–935.CrossRefGoogle Scholar
  17. 17.
    Rodenhuis, S., Slebos, RJ., Boot, A.J.M., Evers, S.G., Mooi, W.J., Wagenaar, S.S., van Bodegom, P.C., and Bos, J.L. (1988) Submitted.Google Scholar
  18. 18.
    Lemoine, N.R., Mayall, E.S., Wyllie, F.W., Dillwyn-Williams, E., Goyns, M., Stringer, B., and Wynford-Thomas, D. (1989) in press.Google Scholar
  19. 19.
    Suarez, H.G., Du Villard, J.A., Caillou, B., Schlumberger, M., Tubiana, M., Parmentier, C., and Monier, R. (1988) Oncogene 2:403–406.Google Scholar
  20. 20.
    Fusco, A., Grieco, M., Santoro, Berlingieri, M.J., Pilotti, S., Pieroui, M.A., Dell Porta, G., and Vecchio, G. (1987) Nature 328:170–172.CrossRefGoogle Scholar
  21. 21.
    Raybaud, F., Noguchi, T., Marics, I., Adelaide, J., Planche, J., Batoz, M., Aubert, C., de Lapeyriere, O., and Bimbaum, D. (1988) Cancer Res. 48:950–953.Google Scholar
  22. 22.
    Van’t Veer, L.J., Burgering, B.M.Th., Versteeg, R., Boot, A.J.M., Ruiter, DJ., Osanto, S., Schrier, P.I., and Bos, J.L. (1989) Submitted.Google Scholar
  23. 23.
    Raskind, W.H., Triumali, N., Jacobson, R., Singer, J., and Fialkow, P.J. (1984) Blood 63:1318–1323.Google Scholar
  24. 24.
    Nowell, P.C. (1976) Science 194:23–28.CrossRefGoogle Scholar
  25. 25.
    Bos, J.L., Toksoz, D., Marshall, C.J., Verlaan-de Vries, M., Veeneman, G.H., van der Eb, A.J., van Boom, J.H., Janssen, J.W.G., and Steenvoorden, A.C.M. (1985) Nature 315:726–730.CrossRefGoogle Scholar
  26. 26.
    Bos, J.L., Verlaan-de Vries, M., van der Eb, A.J., Janssen, J.W.G., Delwel, R., Lowenberg, B., and Colly, L.P. (1987) Blood 69:1237–1241.Google Scholar
  27. 27.
    Needleman, S.W., Kraus, M.H., Srivastava, S.K., Levine, P.H., and Aaronson, S.A. (1986) Blood 67:753–757.Google Scholar
  28. 28.
    Hirai, H., Kobayashi, Y, Mano, H., Hagiwara, K., Maru, Y., Omine, M., Mizoguchi, H., Nishida, J., and Takaku, F. (1987) Nature 327:430–432.CrossRefGoogle Scholar
  29. 29.
    Janssen, J.W.G., Steenvoorden, A.C.M., Lyons, J., Anger, B., Bohlke, J.U., Bos, J.L., Seliger, H., and Bartram, C.R. (1987) Proc. Natl. Acad. Sci. USA USA 84:9228–9232.CrossRefGoogle Scholar
  30. 30.
    Lui, E., Hjelle, B., Morgan, R., Hecht, F., and Bishop, J.M. (1987) Nature 330:186–188.CrossRefGoogle Scholar
  31. 31.
    Toksoz, D., Farr, C.J., and Marshall, C.J. (1987) Oncogene 1:409–413.Google Scholar
  32. 32.
    Farr, C.J., Saiki, R.K., Ehrlich, H.A., McCormick, F., and Marshall, C.J. Proc. Natl. Acad. Sci. USA 85:1629–1633.Google Scholar
  33. 33.
    Lyons, J., Janssen, J.W.G., Bartram, C.R., Layton, D.M., and Mufti, G.J. (1988) Blood 71:1707–1711.Google Scholar
  34. 34.
    Senn, H.P., Fopp, J.M., Schmid, L., and Moroni, C. (1988) Int. J. Cancer 41:59–64.CrossRefGoogle Scholar
  35. 35.
    Padua, R.A., Carter, G., Hughes, D., Gow, J., Farr, C., Oscier, D., McCormick, F., and Jacobs, A. (1988) Leukemia 2:503–510.Google Scholar
  36. 36.
    Yunis, JJ., Boot, A.J.M., Mayer, M.G., and Bos, J.L. (1988) Submitted.Google Scholar
  37. 37.
    Senn, H.P., Fopp, J.M., Schmid, L., and Moroni, C. (1988) Blood 72:931–935.Google Scholar
  38. 38.
    Kraus, M.H., Yuasa, Y., and Aaronson, S.A. (1984) Proc. Natl. Acad. Sci, USA 81:5384–5388.CrossRefGoogle Scholar
  39. 39.
    Van de Vijver, M., Peterse, J.L., Mooi, W.J., Lohmans, J., Verbruggen, M., van de Bersselaar, R., Devilee, P., Cornelisse, C., Bos, J.L., Yarnolad, J., and Nusse, R. (1988) Cancer Cell in press.Google Scholar
  40. 40.
    Spandidos, D.A. (1987) Anticancer Res. 7:991–996.Google Scholar
  41. 41.
    Van’t Veer, L.J., Hennens, R., van den Berg-Bakker, L.A.M., Cheng, N. Ching, Fleuren, G.J., Bos, J.L., Cleton, F.J., and Schrier, P.I. (1988) Oncogene 2:157–165.Google Scholar
  42. 42.
    Hollstein, M.C., Smits, A.M., Galiana, C., Yamasaki, H., Bos, J.L., Mandard, A., Partensky, C., and Montesano, R. (1988) Submitted.Google Scholar
  43. 43.
    Ballas, K., Lyons, J., Janssen, J.W.G., and Bartram, C.R. (1988) Eur. J. Ped. 147:313–314.CrossRefGoogle Scholar
  44. 44.
    Sakato, H., Mori, M., Taira, M., Yoshida, T., Matsukawa, S., Shimizu, K., Sekiguchi, M., Terada, M., and Sugimura, T. (1986) Proc. Natl. Acad. Sci. USA 83:3997–4001.CrossRefGoogle Scholar
  45. 45.
    Fujita, K.N., Ohuchi, T. Yao, Okumura, M., Fukushima, Y., Kanakura, V., Kitamura, Y., and Fujita, J. (1987) Gastroenterology 6:1339–1345.Google Scholar
  46. 46.
    Fujita, J., Kraus, M.H., Onoue, H., Srivastava, S.K., Ebi, Y., Kitamura, Y., and Rhim, J.S. (1988) Cancer Res. 48:5251–5255.Google Scholar
  47. 47.
    Leon, J., Kamino, H., Steinberg, J.J., and Pellicer, A. (1988) Mol. Cell. Biol. 8:786–793.CrossRefGoogle Scholar
  48. 48.
    Fujita, J., Srivastava, S.K., Kraus, M.H., Rhim, J.S., Tronick, S.R., and Aaronson, S.A. (1985) Proc. Natl. Acad. Sci. USA 82:3849–3853.CrossRefGoogle Scholar
  49. 49.
    Malone, P.R., Visvanathan, K.V., Ponder, BA., Shearer, R.J., and Summerhayes, II.C. (1985) Br. J. Urol. 57:664–667.CrossRefGoogle Scholar
  50. 50.
    Visvanathan, K.V., Pocock, R.D., and Summerhays, I.C. (1988) Oncogene Res. 3:77–86.Google Scholar
  51. 51.
    Liu, E., Hjelle, B., and Bishop, J.M. (1988) Proc. Natl. Acad. Sci. USA 85:1952–1956.CrossRefGoogle Scholar
  52. 52.
    Rodenhuis, S., Bos, J.L., Slater, R.M., Behrendt, H., van’t Veer, M., and Smets, L.A. (1986) Blood 67:1698–1704.Google Scholar
  53. 53.
    Neri, A., Knowles, D.M., Greco, A, McCormick, F., and Dalla-Favera, R. (1988) Proc. Natl. Acad. Sci. USA 85:9268–9272CrossRefGoogle Scholar

Copyright information

© Stockton Press 1989

Authors and Affiliations

  • Johannes L. Bos

There are no affiliations available

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