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Purification, characterization and reconstitution of the adenylate cyclase-coupled β-adrenergic receptor

  • R. J. Lefkowitz
  • R. A. Cerione
  • J. L. Benovic
  • B. Strulovici
  • G. L. Stiles
  • J. Codina
  • L. Birnbaumer
  • M. G. Caron

Abstract

Of all the receptor-coupled effector systems found in mammalian cells none has received more attention than the adenylate cyclase system (Lefkowitz et al., 1983; Smigel et al., 1984). Its ubiquity and the diversity of hormones and drugs which stimulate it has made it a premier model system for the study of the mechanisms whereby drugs and hormones activate their physiological effectors. Although numerous hormone receptors are coupled in a stimulatory or inhibitory fashion to the adenylate cyclase, to date only the β-adrenergic receptors, which respond to catecholamines such as adrenaline and noradrenaline, have been characterized in any significant detail. They are the only adenylate cyclase-coupled receptors which have been purified (Lefkowitz et al., 1983; Stiles et al., 1984a).

Keywords

Adenylate Cyclase Adrenergic Receptor GTPase Activity Adenylate Cyclase System Competition Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Macmillan Publishers Limited 1984

Authors and Affiliations

  • R. J. Lefkowitz
    • 1
  • R. A. Cerione
    • 1
  • J. L. Benovic
    • 1
  • B. Strulovici
    • 1
  • G. L. Stiles
    • 1
  • J. Codina
    • 2
  • L. Birnbaumer
    • 2
  • M. G. Caron
    • 1
  1. 1.Howard Hughes Medical Institute, Departments of Medicine, Biochemistry and PhysiologyDuke University Medical CenterDurhamUSA
  2. 2.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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