Advertisement

Gene structure of the pulmonary surfactant proteins SP-A, SP-B and SP-C

  • Jeffrey A. Whitsett
Chapter

Abstract

The application of molecular biology to the study of pulmonary surfactant has resulted in the isolation and characterization of the genes encoding surfactant proteins SP-A, SP-B and SP-C. Nucleotide sequences of the genes provide a precise amino acid sequence of the polypeptides and a starting point for the elucidation of the genetic elements, acting in cis, which may control lung-specific, developmental and hormonal control of surfactant protein synthesis. The present summary will provide an overview of recent progress made in the analysis of the surfactant protein genes, primarily describing work from our laboratory.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    White, R. T., Damm, D., Miller, J., Spratt, K., Schilling, J., Hawgood, S., Benson, B. and Cordell, B. Isolation and characterization of the human pulmonary surfactant apoprotein gene. Nature 1985; 317: 361–3.CrossRefGoogle Scholar
  2. [2]
    Korfhagen, T. R.,Glasser, S. W., Bruno, M. D., Clark, J. C., Pilot-Matias T. J. and Whitsett, J. A. Complexity of human pulmonary SP-A gene sequences. Pediatr Res 1988; 23: 513A (Abstr).CrossRefGoogle Scholar
  3. [3]
    Hawgood, S. Pulmonary surfactant apoproteins: a review of protein and genomic structure. Am J Physiol: Lung, Cell Mol Physiol 1989; 1: 13–22.Google Scholar
  4. [4]
    Whitsett, J. A. and Weaver, T. E. Pulmonary surfactant proteins: implications for surfactant replacement therapy. In: Shapiro, D. L. and Notter, R. H. eds. Surfactant Replacement Therapy, New York: Alan R. Liss, 1989; 71–89.Google Scholar
  5. [5]
    Pilot-Matias, T. J., Kister, S. E., Fox, J. L., Kropp, K., Glasser, S. W. and Whitsett, J. A. Structure and organization of the gene encoding human pulmonary surfactant proteolipid SP-B. DNA 1989; 8: 75–86.CrossRefGoogle Scholar
  6. [6]
    Whitsett, J. A., Weaver, T. E., Clark, J. C., Sawtell, N., Glasser, S. W., Korfhagen, T. R. and Hull, W. M. Glucocorticoid enhances surfactant proteolipid Phe and pVal synthesis and RNA in fetal lung. J Biol Chem 1987; 262: 15618–23.Google Scholar
  7. [7]
    Glasser, S. W., Korfhagen, T. R., Perme, C. M., Pilot-Matias, T. J., Kister, S. E and Whitsett, J. A. Two genes encoding human pulmonary surfactant proteolipid SP-C. J Biol Chem 1988; 263: 10326–31.Google Scholar

Copyright information

© Macmillan Publishers Limited 1991

Authors and Affiliations

  • Jeffrey A. Whitsett

There are no affiliations available

Personalised recommendations