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In Vivo Methods for the Rate of Serotonin Synthesis and Axonal Transport Measurements in the Brain

  • Mirko Diksic
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Abstract

For more than twenty years researchers have been searching for a method of measuring the rate of brain serotonin synthesis which is both convenient and anatomically precise. Serotonin (5-hydroxytryptamine; 5-HT) is a neuro-transmitter widely distributed in the brain. It has been implicated in many brain functions (e.g. sleep cycle: Jouvet, 1967; food intake: Blundell and Hill, 1987) and in disorders ranging from migraine (e.g. Andersen and Dafny, 1983) to schizophrenia (e.g. Sedvall, 1981) and depression (e.g. Young et al., 1981). To date it has been impossible to measure serotonin synthesis rate in living human brain, although it has often been determined in laboratory animals in a ‘normal’ state and under the influence of various drugs. All procedures developed to date are fatal and therefore obviously not useful in humans. Attempts have been made to measure the neurotransmitter concentration in post-mortem human brain (e.g. Dodd et al., 1988). However, although these measurements might provide information on the steady state concentration of 5-HT, they cannot give any information about the 5-HT synthesis rate. And, of course, there is always the possibility of post-mortem changes.

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Copyright information

© Macmillan Publishers Limited 1991

Authors and Affiliations

  • Mirko Diksic
    • 1
    • 2
  1. 1.Department of Neurology and NeurosurgeryMcGill UniversityMontrealCanada
  2. 2.Montreal Neurological Institute and HospitalCanada

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