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GABAergic Neurones and Terminals in the Dorsal Column Nuclei and the Lateral Cervical Nucleus

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Information Processing in the Somatosensory System

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Abstract

More than 20 years ago Galindo and co-workers (1967) found that extracellular micro-iontophporetic application of y-aminobutyric acid or GABA had substantial inhibitory effects on cuneate neurones. Subsequently, several investigators using different techniques (Penny et al.,1983, Rustioni et al.,1983, Spreafico et al.,1983, Newberry and Simmonds, 1984, Rustioni et al., 1984, Westman et al., 1984, Blomqvist et.al., 1985, Ralston, 1985, Basbaum et al., 1986, Barbesi et al., 1986, Simmonds, 1987, Matthews et al., 1988) published reports emphasizing the role of GABA as an inhibitory transmitter at different subcortical levels of the somatosensory systems. The immuno-cytochemistry of these studies employed initially an indirect method to demonstrate GABA using antiserum against glutamic acid decarboxylase (GAD), the enzyme responsible for the synthesis of GABA.

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References

  • Andersen, P. Eccles, J.C. Schmidt, R. F. and Yokota, T. (1964a). Identification of relay cells and interneurones in the cuneate nucleus. J. Neurophysiol., 27, 1080–1095.

    CAS  PubMed  Google Scholar 

  • Andersen, P. Eccles, J.C. Oshima, T. and Schmidt, R. F. (1964b). Mechanisms of synaptic transmission in the cuneate nucleus. J. Neurophysiol., 27, 1096–1116.

    CAS  PubMed  Google Scholar 

  • Barbaresi, P. Spreafico, R. Frassoni, C. and Rustioni, A. (1986). GABAergic neurons are present in the dorsal column nuclei but not in the ventroposterior complex of rats. Brain Res 382, 305–326,.

    Article  CAS  PubMed  Google Scholar 

  • Basbaum, A I. Glazer, E. J. and Oertel, W. (1986). Immunoreactive glutamic acid decarboxylase in the trigeminal nucleus caudalis of the cat: a light-and electron-microscopic analysis. Somatosens. Res., 4, 77–94.

    Article  CAS  PubMed  Google Scholar 

  • Blomqvist, A. and Westman, J. (1976). Interneurons and initial axon collaterals in the feline gracile nucleus demonstrated with the rapid Golgi technique. Brain Res., 111, 407–410.

    Article  CAS  PubMed  Google Scholar 

  • Blomqvist, A. Westman, J., Köhler, J. and Wu, J-Y. (1985). Immunocytochemical localization of glutamic acid decarboxylase and substance P in the lateral cervical nucleus: A light and electron microscopic study in the cat. Neurosci. Lett., 56, 229–233.

    Article  CAS  PubMed  Google Scholar 

  • Boone, T. B. and Aides, L. D. (1984). The ultrastructure of two distinct neuron populations in the hypoglossal nucleus of the rat. Exp. Brain Res., 54, 321–326.

    Article  CAS  PubMed  Google Scholar 

  • Brodal, A. (1941). Die Verbundungen des Nucleus Cuneatus Externus mit den Kleinhirn beim Kaninchen und bei der Katze. Z. ges. Neurol. Psychiat., 171, 167–199.

    Article  Google Scholar 

  • Broman, J. and Blomqvist, A. (1989). GABA-Immunorective neurones and terminals in the lateral cervical nucleus of the cynomolgus monkey. J. Comp. Neurol. 283. 415–424.

    Article  CAS  PubMed  Google Scholar 

  • Broman, J. and Westman, J. (1988). GABAergic neurons and terminals in the lateral cervical nucleus of the cat. J. Comp Neurol., 274, 461–472.

    Google Scholar 

  • Cavalieri, B. (1635 and 1966) Geometrica degli Invisibili. Torino: UnioneTipografico-Editrice Torinese.

    Google Scholar 

  • Craig, A.D. Jr. and Tapper, D.N. (1978). Lateral cervical nucleus in the cat: Functional organization and characteristics. J. Neurophysiol. 41, 1511–1534.

    PubMed  Google Scholar 

  • Craig, A.D. Jr. Sailer, S. and Kniffki, K.-D.(1987). Organization of anterogradely labeled spinocervical tract terminations in the lateral cervical nucleus of the cat. J. Comp. Neurol. 263. 214–222.

    Article  PubMed  Google Scholar 

  • Cullheim, S. and Kellerth, J. O. (1978). Combined light and electron microscopic tracing of neurons, including axons and synaptic terminals after intracellular injection of horseradish peroxidase. Neurosci. Lett., 2, 307–313.

    Article  Google Scholar 

  • Dale, N. Ottersen, O. P. Roberts, A. and Storm-Mathisen, J. (1986) Inhibitory neurons of a motor patterns generator in Xenopus revealed by antibodies to glycin. Nature 324. 255–257.

    Article  CAS  PubMed  Google Scholar 

  • Destombes, J. Gogan, P. and Rouviere, A. (1979). The fine structure of neurones and cellular relationsships in the abducens nucleus in the cat. Exp. Brain. Res., 35, 249–267.

    Article  CAS  PubMed  Google Scholar 

  • Elias, H. and Schwartz, D. (1971). Cerbrocortical surface areas, lengths of gyri and their interdependence in mammals, including man. Z. Säugetirk., 36, 147–163.

    Google Scholar 

  • Enevoldson, T. P. Gordon, G. and Sanders, D.J. (1984). The use of retrograde transport of horseradish peroxidase for studying the dendritic trees and axonal courses of particular groups of tract cells in the spinal cord. A modified procedure and its evaluation in the cat and rat. Exp. Brain Res., 54, 529–537.

    CAS  PubMed  Google Scholar 

  • Falls, W. M. and King, J. S. (1976). The facial motor nucleus of the opossum: Cytology and axosomatic synapses. J. Comp. Neurol., 167. 177–204.

    Article  CAS  PubMed  Google Scholar 

  • Fedina, L. Gordon, G. and Lundberg, A. (1968). The source and mechanisms of inhibition in the lateral cervical nucleus of the cat. Brain Res 11, 694–696.

    Article  CAS  PubMed  Google Scholar 

  • Ferraro, A. and Barrera, S. E. (1935). The nuclei of the posterior funiculi in Macacus rhesus. An anatomic and experimental investigation. Arch. Neurol. Psychiat. (Chic.) 33, 262–275.

    Article  Google Scholar 

  • Flink, R. and Westman, J. (1986). Different neuron populations in the feline lateral cervical nucleus: A light and electron microscopical study with the retrograde axonal transport technique. J. Comp. Neurol., 250, 265–281.

    Article  CAS  PubMed  Google Scholar 

  • Gabbot, P. L. A. Somogyi, J. Stewart, M. G. and Hamori, J. (1986). A quantitative investigation of neuronal composition of the rat dorsal lateral geniculate nucleus using GABA-immunocytochemistry. Neuroscience 19. 101–111.

    Article  Google Scholar 

  • Galindo, A. Krnjevic, K. and Schwartz, S. (1967). Micro-iontophoretic studies on neurones in the cuneate nucleus. J. Physiol. (Lond.)., 192. 359–377.

    Article  CAS  Google Scholar 

  • Glagolev, A.A. (1934). Quantitative analysis with the microscope by the point method. Eng. Min. J., 135, 399–402.

    CAS  Google Scholar 

  • Giesler, G.J. Jr., and Elde, R.T. (1985). Immunocytochemical studies of the peptidergic content of fibers and terminals within the lateral spinal and lateral cervical nuclei. J. Neurosci., 5, 1833–1841.

    CAS  PubMed  Google Scholar 

  • Gordon, G., and Jukes, M.G.M. (1963). An investigation of cells in the lateral cervical nucleus of the cat which respond to stimulation of the skin. J. Physiol. (Lond.) 169, 28–29.

    Google Scholar 

  • Gundersen, H. J. G. Bagger, P. Bendtsen, T. F. Evans, S. M. Korbo, L. Marcussen, N. Møller, A. Nielsen, K. Nyengaard, J. R. Pakkenber, G. B. Sørensen, F. B. Vesterby, A. and West, M. J. (1988). The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological reasearch and diagnosis. APMIS 96, 857–881.

    Article  CAS  PubMed  Google Scholar 

  • Heino, R. & Westman, J.(1990). Quantitative analysis of the feline dorsal column nuclei and their GABAergic and non-GABA-ergic neurons. Anat. Embryol. Submitted.

    Google Scholar 

  • Horrobin, D.F. (1966). The lateral cervical nucleus of the cat; an electrophysiological study. Q. J. Exp. Physiol., 51. 351–371.

    Article  CAS  PubMed  Google Scholar 

  • Jankowska, E. Rastad, J. and Westman, J. (1976). Intracellular application of horseredish peroxidase and its light and electron microscopical appearence in spinocervical tract cells. Brain Res. 105, 557–562.

    Article  CAS  PubMed  Google Scholar 

  • Kosaka, T. Nagatsu, I. Wu, J.-Y. and Hama, K. (1986). Use of high concentrations of glutaraldehyde for immunocytochemistry of transmitter-synthesizing enzymes in the central nervous system. Neuroscience 18, 975–990.

    Article  CAS  PubMed  Google Scholar 

  • Kuypers, H. G. J. M. and Tuerk, J. D. (1964). The distribution of cortical fibres within the nuclei cuneatus and gracilis in the cat. J. Anat. Lond., 98, 143–162.

    CAS  Google Scholar 

  • Lieberman, A. R. (1973). Neurons with presynaptic perikarya and presynaptic dendrites in the rat lateral geniculate nucleus. Brain Res., 59, 35–59.

    Article  CAS  PubMed  Google Scholar 

  • Madarász, M., Somogyi, Gy. Somogyi, J and Hámori, J.(1985). Numerical estimation of y-aminobutyric acid (GABA)-containing neurons in three thalamic nuclei of the cat: direct GABA immunocytochemistry. Neurosci. Lett. 61, 73–78.

    Article  PubMed  Google Scholar 

  • Matthews, M. A. McDonald, G. K. and Hernandez, T. V. (1988). GABA distribution in a pain-modulating zone of trigeminal subnucleus interpolaris. Somatosens. Res., 5, 205–217.

    Article  CAS  PubMed  Google Scholar 

  • Newberry, N. R. and Simmonds, M. A. (1984). The rat gracile nucleus in vitro: I. Evidence for GABA-mediated depolarisation of the dorsal column afferents. Brain Res., 303. 41–49.

    Article  CAS  PubMed  Google Scholar 

  • OHara, P.T. Lieberman, A. R. Hunt, S. P. and Wu, J-Y. (1983). Neural elements containing glutamic acid decarboxylase (GAD) in the dorsal lateral geniculate nucleus of the rat: immunohistochemical studies by light and electron microscopy. Neuroscience, 8, 189–211.

    Article  Google Scholar 

  • OHara, P.T. Chazal, G. and Ralston, H.J. III (1989). Ultrastructural analysis of GABA-immunoreactive elements in the monkey thalamus ventrobasal complex. J. Comp. Neurol., 283. 541–558

    Article  Google Scholar 

  • O’Neal, J.T. & Westrum, L.E.: The fine structural synaptic organisation of the cat lateral cuneate nucleus. A study of sequential alterations in degeneration. Brain. Res., 51, 97–124, 1973.

    Article  PubMed  Google Scholar 

  • Ottersen, O. P. Storm-Mathisen, J., Madsen, S. Skumlien, S. and Strømhaug, J. (1986). Evaluation of the immunocytochemical method for amino acids. Medical Biology 64, 147–158.

    CAS  PubMed  Google Scholar 

  • Penny, G. R. Fitzpatrick, G., Schmechel, D. E. & Diamond, I.T (1983). Glutamic acid decarboxylase-immunoreactive neurons and horseradish peroxidase labeled projection neurons in the ventral posterior nucleus of the cat and Galago senegalensis. J. Neurosci., 3, 1868–1887.

    CAS  PubMed  Google Scholar 

  • Rafols, J. A. and Fox, C. A. (1976). The neurons in the primate subthalamic nucleus: A Golgi and electron microscopical study. J. Comp. Neurol., 168, 75–112.

    Article  CAS  PubMed  Google Scholar 

  • Ralston, H. J. III (1985). The fine structure of the ventrobasal thalamus of the monkey and cat. In: Albe-Fessard D. Berkley, K. J. Kruger, L. Ralston, H. J. III, Willis, W. D. Jr. Diencephalic mechanisms of pain sensation. Brain. Res. Rev., 9, 228–241

    Google Scholar 

  • Rinvik, E., Ottersen, O. P. and Storm-Mathisen, J (1987). Gamma-aminobutyrate-like immunoreactivity in the thalamus of the cat. Neuroscience 21, 781–805,.

    Article  CAS  PubMed  Google Scholar 

  • Rustioni, A. Schmechel, D. E. Spreaflco, R. Cheema, S. and Cuénod, M. (1983). Excitatory and inhibitory amino acid putative neurotransmitters in the ventralis posterior complex: an autoradiographic and immunocytochemical study in rats and cats. In: Macchi, G. Rustioni, A. Spreaflco, R. Somatosensory integration in the thalamus. Elsevier, Amsterdam pp 365–383.

    Google Scholar 

  • Rustioni, A. Schmechel, D. E., Cheema, S. and Fitzpatrick, D. (1984). Glutamic acid decarboxylase-containing neurons in the dorsal column nuclei of the cat. Somatosens. Res., 1: 329–357.

    Article  CAS  PubMed  Google Scholar 

  • Sakumoto, T. Nagai, T. Kimura, H. and Maeda, T. (1980). Electron microscopic visualization of tetramethyl benzidine reaction product on horseradish peroxidase neurohistochemistry. Cell Mol. Biol., 26, 211–216.

    CAS  Google Scholar 

  • Schönitzer, K. and Holländer, H. (1981). Anterograde tracing of horseradish peroxidase (HRP) with the electron microscope using the tetramethyl benzidine reaction. J. Neurosci. Methods 4, 373–383.

    Article  PubMed  Google Scholar 

  • Simmonds, M. A. (1987). Classification of inhibitory amino acid receptors in the mammalian nervous system. Med. Biol. 64, 301–311.

    Google Scholar 

  • Spreaflco, R. Schmechel, D. E., Ellis, L. C. Jr. and Rustioni, A. (1983). Cortical relay neurons and interneurons in the N. ventralis posterolateral of cats: a horseradish peroxidase, electron-microscopic, Golgi and immunocytochemical study. Neuroscience 9, 491–509.

    Article  Google Scholar 

  • Sterio, D.C. (1984). The unbiased estimation of number and sizes of arbitrary particles using the disector. J. Microscopy 134. 127–136.

    Article  CAS  Google Scholar 

  • Stürmer, C. Bielenberg, K. and Spatz, W. B. (1981). Electron microscopical identification of 3, 3′, 5, 5′-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport. Neurosci. Lett. 23, 1–5.

    Article  PubMed  Google Scholar 

  • Svensson, B. A. Westman, J. and Rastad, J. (1985). Light and electron microscopic study of neurones in the feline lateral cervical nucleus with a descending projection. Brain Res., 361. 114–124.

    Article  CAS  PubMed  Google Scholar 

  • Svensson, B. A. Griph, S. Rastad, J. and Westman, J. (1987). Quantitative ultrastructural study of boutons of ascending afferents to the feline lateral cervical nucleus. Brain Res., 423. 229–226.

    Article  CAS  PubMed  Google Scholar 

  • Walberg, F. (1965). Axo-axonic contacts in the cuneate nucleus, probable basis for presynaptic depolarisation. Exp.Neurol 13, 218–231.

    Article  CAS  PubMed  Google Scholar 

  • Westman, J. (1971a). Quantitative estimation of the proportion of perikaryal surface area covered with boutons — a possibility to distinguish different nerve cell populations. Brain Res., 32, 203–207.

    Article  CAS  PubMed  Google Scholar 

  • Westman, J. (1971b). The lateral cervical nucleus in the cat. V. A quantitative evaluation on the bouton-and glia-covered surface area of different LCN-neurons. Z. Zeilforsch., 115, 377–387.

    Article  CAS  Google Scholar 

  • Westman, J (1989a). Light and electron microscopical studies of the Substance P innervation of the dorsal column nuclei and the lateral cervical nucleus in the primate species Macaca fascicularis and Papio anubis. Uppsala J. Med. Sci., 94, 123–128.

    Article  CAS  Google Scholar 

  • Westman, J (1989b). Light and electron microscopical studies of the GABA innervation of the dorsal column nuclei and the lateral cervical nucleus in the primate species Macaca fascicularis and Papio anubis. Uppsala J. Med. Sci., 94, 255–270.

    Article  CAS  Google Scholar 

  • Westman, J. Blomqvist, A. Köhler, C. and Wu, J-Y. (1984). Light and electron microscopic localization of glutamic acid decarboxylase and substance P in the dorsal column nuclei of the cat. Neurosci. Lett., 51, 347–35.

    Article  CAS  PubMed  Google Scholar 

  • Westman, J, Heino, R. and Dietrichs, E. (1989). GABA immunorectivity in the human dorsal column nuclei. Neuvieme Séminaire Hivernal des Neurosciences Européennes, Les Arcs (France) p 41.

    Google Scholar 

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  1. Jan Westman
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  1. Department of Human Anatomy, University of Uppsala, Sweden

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Westman, J. (1991). GABAergic Neurones and Terminals in the Dorsal Column Nuclei and the Lateral Cervical Nucleus. In: Franzén, O., Westman, J. (eds) Information Processing in the Somatosensory System. Wenner-Gren Center International Symposium Series. Palgrave, London. https://doi.org/10.1007/978-1-349-11597-6_9

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  • DOI: https://doi.org/10.1007/978-1-349-11597-6_9

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