Generating Immortalized Immunoglobulin-secreting Human Lymphocytes by Recombinant DNA Technology

  • Martin I. Mally
  • Mark C. Glassy

Abstract

The classical technique of producing hybridomas involves the fusion of B lymphocytes, albeit at a low efficiency (approximately 1 hybrid per 105–106 cells), with an appropriate fusion partner, typically a myeloma or lymphoblastoid cell line, via the cumbersome and somewhat unpredictable polyethylene glycol (PEG) procedure.1 The fusion partner simply acts as a vector, supplying immortalization functions to an immunoglobulin-secreting lymphocyte. Recently, however, other approaches have become available that have the potential to generate immortalized, immunoglobulin-secreting lymphocytes with a higher efficiency. The aim of this chapter is to provide an introduction to an alternative method of generating immortalized lymphocytes—that of a form of DNA-mediated gene transfer known as electroporation.

Keywords

Permeability Sucrose Lymphoma Titration Rubber 

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Copyright information

© Palgrave Macmillan, a division of Macmillan Publishers Limited 1989

Authors and Affiliations

  • Martin I. Mally
  • Mark C. Glassy

There are no affiliations available

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