Antibodies have proved themselves to be extremely versatile and sensitive reagents for the detection and quantitation of an analyte. This is partly because the mechanisms for generating antibody diversity ensure that an immense range of different chemical structures can be recognised, with exquisite specificity, by the antibody population. This allows the analyst to select an individual antigen-targeted antibody and use it to distinguish the antigen from a complex background of closely related structures. Furthermore, the recognition properties of the antibody can be readily coupled to a detection mechanism which can quantitatively signal antigen binding by the antibody. There are now a wide-range of different physical and chemical detection mechanisms which can be employed to produce such a signal.
KeywordsFermentation Recombination Adduct Oligomer Polypeptide
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