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A Novel Homogeneous β-Galactosidase Immunoassay System

  • Dan Monroe

Abstract

A new generation of enzyme immunoassays (EIAs) that offer better accuracy is now possible with the help of recombinant DNA techniques. The use of fragmented β-galactosidase proteins obtained from genetically engineered Escherichia coli forms the basis of this new concept in EIAs. Traditionally, EIAs have been performed using whole enzyme molecules covalently linked to an antibody, an antigen, or a hapten, as described elsewhere (Monroe, 1983, 1984, 1985). Assay speed and precision are often compromised with conventional methods, however, owing to required sample pretreatment, incubation, and washing steps. Such tedious procedures can now be eliminated by means of this new separation-free or homogeneous β-galactosidase immunoassay system known as CEDIA™ (Henderson et al., 1986).

Keywords

Tetrameric Enzyme Enzyme Peptide Sodium Potassium Phosphate Homogeneous Immunoassay Enzyme Donor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Editor and Contributors 1989

Authors and Affiliations

  • Dan Monroe

There are no affiliations available

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