A Novel Homogeneous β-Galactosidase Immunoassay System
A new generation of enzyme immunoassays (EIAs) that offer better accuracy is now possible with the help of recombinant DNA techniques. The use of fragmented β-galactosidase proteins obtained from genetically engineered Escherichia coli forms the basis of this new concept in EIAs. Traditionally, EIAs have been performed using whole enzyme molecules covalently linked to an antibody, an antigen, or a hapten, as described elsewhere (Monroe, 1983, 1984, 1985). Assay speed and precision are often compromised with conventional methods, however, owing to required sample pretreatment, incubation, and washing steps. Such tedious procedures can now be eliminated by means of this new separation-free or homogeneous β-galactosidase immunoassay system known as CEDIA™ (Henderson et al., 1986).
KeywordsHydrolysis Phosphorus Filtration Acetonitrile Titration
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