Abstract
Light microscopy has entered an exciting era in which conventional imaging with light has achieved unprecedented lateral and vertical resolution that is approaching the fundamental limits that physics imposes on imaging with light focused through a lens. In addition, new lensless techniques of optical imaging are being devised which have already overcome the limitations of these lens-based instruments that operate in the far field (at distances in which the lens and the sample are separated by multiple wavelengths of light). This chapter describes these great strides in instrumentation which have poised the field at the brink of the possibility of assembling an integrated light microscope with the capability to zoom in from low resolutions of x a few hundred to imaging with single-molecule resolution. These exciting developments will without doubt have a tremendous impact in fields ranging from biology to chemistry and physics and even technology.
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© 1991 The Macmillan Press Ltd
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Lewis, A. (1991). The Confluence of Advances in Light Microscopy: CCD, Confocal, Near-field and Molecular Exciton Microscopy. In: Cherry, R.J. (eds) New Techniques of Optical Microscopy and Microspectroscopy. Topics in Molecular and Structural Biology. Palgrave, London. https://doi.org/10.1007/978-1-349-10802-2_2
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DOI: https://doi.org/10.1007/978-1-349-10802-2_2
Publisher Name: Palgrave, London
Print ISBN: 978-1-349-10804-6
Online ISBN: 978-1-349-10802-2
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