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Video and Opto-Digital Imaging Microscopy

  • David M. Shotton
Chapter
Part of the Topics in Molecular and Structural Biology book series (TMSB)

Abstract

The major inherent advantage of the light microscope (LM) over the electron microscope (EM), namely its utility for the study of dynamic processes in living cells, has until recently been severely compromised by its inability to image very weak fluorescent signals or to make visible minute cellular structures with the contrast generation methods available for unstained cytoplasm. One consequence of this has been the dominance of electron microscopic investigations of fixed cells and tissues during the postwar development of cell biology. However, within the last few years the combination of novel optical microscopic techniques with advanced video and digital image processing technology has resulted in dramatic improvements in the quality of light microscopic images, leading to a true renaissance in the use of light microscopy for the imaging of both fixed and living biological specimens.

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