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Establishment of a Very Sensitive Avidin-Biotin ELISA for the Measurement of Human IgG

  • Y. G. Alevy
  • C. M. Blynn

Abstract

It is well established that antibodies of the IgG isotype are important in resisting infections. Serum levels of IgG in normal individuals vary over a wide range, falling between 662–1372 mg/dl in most individuals. Although established ELISA assays have sufficient sensitivity to detect serum IgG levels, a more sensitive ELISA assay is required for the detection of IgG synthesized by human peripheral blood lymphocytes (PBL), in normal and immunodeficient patients.

Keywords

ELISA Assay Optical Density Reading Zymed Laboratory Immunodeficient Patient Coating Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© S. B. Pal and the Contributors 1988

Authors and Affiliations

  • Y. G. Alevy
  • C. M. Blynn

There are no affiliations available

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