Establishment of a Very Sensitive Avidin-Biotin ELISA for the Measurement of Human IgG
It is well established that antibodies of the IgG isotype are important in resisting infections. Serum levels of IgG in normal individuals vary over a wide range, falling between 662–1372 mg/dl in most individuals. Although established ELISA assays have sufficient sensitivity to detect serum IgG levels, a more sensitive ELISA assay is required for the detection of IgG synthesized by human peripheral blood lymphocytes (PBL), in normal and immunodeficient patients.
KeywordsELISA Assay Optical Density Reading Zymed Laboratory Immunodeficient Patient Coating Antibody
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© S. B. Pal and the Contributors 1988