Establishment of a Very Sensitive Avidin-Biotin ELISA for the Measurement of Human IgG

  • Y. G. Alevy
  • C. M. Blynn


It is well established that antibodies of the IgG isotype are important in resisting infections. Serum levels of IgG in normal individuals vary over a wide range, falling between 662–1372 mg/dl in most individuals. Although established ELISA assays have sufficient sensitivity to detect serum IgG levels, a more sensitive ELISA assay is required for the detection of IgG synthesized by human peripheral blood lymphocytes (PBL), in normal and immunodeficient patients.


ELISA Assay Optical Density Reading Zymed Laboratory Immunodeficient Patient Coating Antibody 
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  1. Bayer, E. A. and Wilchek, M. (1978). The avidin-biotin complex as a tool in molecular biology. Trends Biochem. Sci., 3, N257–N259.Google Scholar
  2. Bayer, E. A. and Wilchek, M. (1980). The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem. Anal., 26, 1–45.CrossRefGoogle Scholar
  3. Green, N. M. (1983). Avidin 1. The use of [14C]biotin for kinetic studies and for assay. Biochem. J., 89, 595.Google Scholar
  4. Guesdon, J., Ternynck, T. and Avrameas, S. (1979). The use of avidin-biotin interaction in immunoenzymatic techniques. J. Histochem. Cytochem., 27, 1131.CrossRefGoogle Scholar
  5. Kendall, C., Ionescue-Matiu, I. and Dressman, G. R. (1983). Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA). J. Immunol. Methods, 56, 329.CrossRefGoogle Scholar
  6. Wilchek, M. and Bayer, E. A. (1984). The avidin-biotin complex in immunology. Immunol. Today, 5, 39.CrossRefGoogle Scholar

Copyright information

© S. B. Pal and the Contributors 1988

Authors and Affiliations

  • Y. G. Alevy
  • C. M. Blynn

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