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Characterization of 5-HT1 Binding Site Subtypes Labelled by [3H]-5-Hydroxytryptamine

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Serotonin

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Abstract

The first radioligand used in an attempt to label receptors for 5-hydroxytryptamine (5-HT) receptors in the CNS was [3H]-5-HT (March-banks, 1966). It was not until 1976, however, that a specific population of high-affinity, saturable binding sites in brain membranes was identified using this radioligand by Bennett and Snyder (1976). In 1979, it was determined that the binding sites labelled by [3H]-5-HT were distinct from a second class of 5-HT binding sites labelled by [3H]-spiperone (Peroutka and Snyder, 1979; Peroutka et al., 1981). The sites labelled by [3H]-5-HT were designated 5-HT1 binding sites, and initially were believed to be homogeneous.

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Author information

Authors and Affiliations

  1. Departments of Neurology and Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA

    S. J. Peroutka

Authors
  1. S. J. Peroutka
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Editor information

Editors and Affiliations

  1. Department of Pharmacology, University of Sydney, Australia

    Ewan J. Mylecharane

  2. Baker Medical Research Institute, Prahran, Australia

    James A. Angus

  3. Department of Clinical and Experimental Pharmacology, University of Adelaide, Australia

    Ivan S. de la Lande

  4. Glaxo Group Research Ltd, Ware, England

    Patrick P. A. Humphrey

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© 1989 The editors and contributors

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Peroutka, S.J. (1989). Characterization of 5-HT1 Binding Site Subtypes Labelled by [3H]-5-Hydroxytryptamine. In: Mylecharane, E.J., Angus, J.A., de la Lande, I.S., Humphrey, P.P.A. (eds) Serotonin. Satellite Symposia of the IUPHAR 10th International Congress of Pharmacology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-10114-6_24

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