Advertisement

D1 and D2 Dopamine Receptors: Identification by Photoaffinity Labeling and Purification by Affinity Chromatography

  • Marc G. Caron
  • Susan E. Senogles
  • Nourdine Amlaiky
  • Joel G. Berger
Part of the Satellite Symposia of the IUPHAR 10th International Congress of Pharmacology book series (SSNIC)

Abstract

In an attempt to characterize the receptors which mediate the physiological actions of dopamine, we have developed several probes for the characterization of both the D1 and D2 subtypes of dopamine receptors. To identify the ligand binding site of these receptors we have developed affinity and photoaffinity probes based on the structure of high affinity selective antagonists. [125I]p(azidcphenethyl) spiperone has been used to identify the ligand binding site of the D2 dopamine receptor in several tissues (Amlaiky and Caron, 1985). More recently, our laboratory has also characterized the ligand binding subunit of the D1 dopamine by covalently incorporating a radioiodinated arylamine derivative of the selective D1 antagonist SCH-23390 into rat striatal membranes by photoaffinity crosslinking (Amlaiky et al. 1987). Another derivative of the potent D2 antagonist spiperone (carboxynethyleneoximinospiperone) has been used in the development of an affinity chromatography procedure for the purification of the D2 receptor from bovine anterior pituitary gland (Senogles et al., 1986).

Keywords

Dopamine Receptor Receptor Preparation Radiation Inactivation Affinity Chromatography Procedure Photoaffinity Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amlaiky, N., Kilpatrick, B.F. and Caron, M.G. (1984) A novel radioiodinated high affinity ligand for the D2-dopamine receptor. FEBS Lett. 176, 436–440.CrossRefPubMedGoogle Scholar
  2. Amlaiky, N. and Caron, M.G. (1985) Photoaffinity labeling of the D2-dopamine receptor using a novel high affinity radioiodinated probe. J. Biol. Chem. 260, 1983–1986.PubMedGoogle Scholar
  3. Amlaiky, N. and Caron, M.G. (1986) Identification of the D2-dopamine receptor binding subunit in several mammalian tissues and species by photoaffinity labeling. J. Neurochem. 47, 196–204.CrossRefPubMedGoogle Scholar
  4. Amlaiky, N., Berger, J.G., Chang, W., McQuade, R.J. and Caron, M.G. (1987) Identification of the binding subunit of the D1-dopamine receptor by photoaffinity crosslinking. Mol. Pharmacol. 31, 129–134.PubMedGoogle Scholar
  5. Bouvier, M., Potier, M., Beauregard, G., Lafond, J., Amlaiky, N., Caron, M.G. and Collu, R. (1986) Solubilization and characterization of the D2-dopamine receptor in estrogen-induced, prolactin-secreting rat pituitary adenoma. J. Neurochem. 47, 1653–1660.CrossRefPubMedGoogle Scholar
  6. Kilpatrick, B.F. and Caron, M.G. (1984) Dopamine receptor of the porcine anterior pituitary gland: Solubilization and characterization. Biochem. Pharmacol. 33: 1981–1988.Google Scholar
  7. Kuno, T. and Tanaka, C. (1983) Correspondency between different affinity states and target size of the bovine striatal D2 dopamine receptor. Biochem. Biophys. Res. Commun. 117, 65–70.CrossRefGoogle Scholar
  8. Lefkowitz, R.J. and Caron, M.G. (1987) Molecular and regulatory properties of adrenergic receptors. Recent Prog. Hormone Res. 43, 469–497.Google Scholar
  9. Lilly, L., Fraser, C.N., Jong, C.Y., Seeman, P. and Venter, J.C. (1983) Molecular size of the canine and human brain D2-dopamine receptor as determined by radiation inactivation. Mol. Pharmacol. 24, 10–14.PubMedGoogle Scholar
  10. Nielsen, M., Klinek, V. and Hyttel, J. (1984) Distinct target size of dopamine D1-and D2 receptors in rat striatum. Life Sci. 35, 325–332.CrossRefPubMedGoogle Scholar
  11. Senogles, S.E., Amlaiky, N., Johnson, A.L. and Caron, M.G. (1986) Affinity chromatography of the anterior pituitary D2-dopamine receptor. Biochem. 25, 749–753.CrossRefGoogle Scholar
  12. Senogles, S.E., Benovic, J.L., Amlaiky, N., Unson, C., Milligan, G., Vinitsky, R., Spiegel, A. and Caron, M.G. (1987) The D2-dopamine receptor of anterior pituitary is functionally associated with a pertussis toxin-sensitive guanine nucleotide binding protein. J. Biol. Chem. 262, 4860–4867.PubMedGoogle Scholar

Copyright information

© Marc G. Caron, Susan E. Senogles, Nourdine Amlaiky and Joel G. Berger 1988

Authors and Affiliations

  • Marc G. Caron
    • 1
  • Susan E. Senogles
    • 1
  • Nourdine Amlaiky
    • 1
  • Joel G. Berger
    • 2
  1. 1.Depts of Physiology and Medicine, Howard Hughes Medical InstituteDuke University Medical CenterDurhamUSA
  2. 2.Pharmaceutical Research DivisionSchering Plough CorpBloomfieldUSA

Personalised recommendations