Purification of Receptors Binding Antipsychotics: Comparison with D2-Dopamine Receptor

  • Y. Nishizawa
  • M. Sato
  • S. Hayashi
  • Y. Takahashi
Part of the Satellite Symposia of the IUPHAR 10th International Congress of Pharmacology book series (SSNIC)

Abstract

Nowadays antipsychotics are essential to treat psychotic patients. Antipsychotics reduce the excitable and stupor conditions, but Parkinsonian tremor, rigidity or dyskinesia appear on the other hand. It seems that the syndrome is dependent on the disturbance of synaptic receptors sensitive to antipsychotics.

Keywords

Sucrose Dopamine Epoxy Fluoride Schizophrenia 

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References

  1. Creese, I., Schneider, R. and Snyder, S.H. (1977) 3H-Spiroperidol Labels Dopamine Receptors in Pituitary and Brain. Eur. J. Pharm., 46, 377–381.CrossRefGoogle Scholar
  2. Gurd, J.W., Jones, J.R., Mahler, H.R. and Moore, W.J. (1974) Isolation and Partial Characterization of Rat Brain Synaptic Plasma membranes. J. Neurochem. 22, 281–290.CrossRefPubMedGoogle Scholar
  3. Kuno, I., Saijoh, K. and Tanaka, C. (1983) Solubilization of D2-Dopamine Receptor Coupled to Guanine Nucleotide Regulatory Protein from Bovine Striatum. J. Neurochem. 41, 841–847.CrossRefPubMedGoogle Scholar
  4. Lowry, O.H., Rosebrough, N.J., Farr, A.R. and Randall, R.J. (1951) Protein Jfeasurement with the Folin Phenol Reagent. J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar
  5. Madras, B., Davis, A., Kunashko, P. and Seeman, P. (1980) Solubilization of Dopamine Receptors from Dog and Human Brains. In Psychopharm. and Biochem. of Neurotransmitter Receptors, (eds. E. Yamamura, H. Olson and E. Usdin) Elsevier North Holland, New York.Google Scholar
  6. Nishizawa, Y., Sato, M., Hayashi, T. and Takahashi, Y. (1986) Purification of Receptors Binding to Antipsychotics — Affinity Chromatography. In Biological Research of Schizophrenia. (ed. K. Inagaki). Sozo Press, Tokyo.Google Scholar
  7. Ramwani, J. and Mishra, R.K. (1986) Purification of Bovine Striatal Dopamine D-2 Receptor by Affinity Chromatography. J. Biol. Chem. 261, 8894–8896.PubMedGoogle Scholar
  8. Riddall, D.R. and Leavens, W.J. (1978) Affinities of Drugs for the Agonist and Antagonist States of the Dopamine Receptor. Eur. J. Pharmacology, 51 187–188.CrossRefGoogle Scholar
  9. Seeman, P. (1980) Brain Dopamine Receptors. Pharmacol. Rev., 32, 229–313.PubMedGoogle Scholar
  10. Senogles, S.E., Amlaiky, N., Johnson, A.L. and Caron, M.G. (1986) Affinity Oiromatography of the Anterior Pituitary D2-Dopamine Receptor. Biochemistry, 25, 749–753.CrossRefPubMedGoogle Scholar

Copyright information

© Y. Nishizawa, M. Sato, S. Hayashi and Y. Takahashi 1988

Authors and Affiliations

  • Y. Nishizawa
    • 1
  • M. Sato
    • 1
  • S. Hayashi
    • 1
  • Y. Takahashi
    • 2
  1. 1.Department of Clinical Investigation and ResearchSaigata National HospitalNakakubikigunJapan
  2. 2.Department of Neuropharmacology, Brain Research InstituteUniversity of NiigataNiigataJapan

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