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Electromechanical and Physicochemical Regulation of Cartilage Strength and Metabolism

  • Chapter
Book cover Connective Tissue Matrix

Part of the book series: Topics in Molecular and Structural Biology ((TMSB))

Abstract

Articular cartilage functions as a weight-bearing wear-resistant tissue in synovial joints. The sparse population of cells in adult articular cartilage is responsible for the synthesis, maintenance and gradual turnover of an extracellular matrix (ECM) composed principally of hydrated collagen fibrils, highly charged proteoglycan molecules (PG) and glycoproteins (Hay, 1981; Muir, 1980). Recent discoveries concerning the genetic expression, metabolism, structure and function of these various matrix constituents have been summarized in excellent reviews (Hunziker and Schenk, 1987; Mecham, 1986; Kuettner et al., 1986; Heinegrd and Paulsson, 1987; Heinegård and Sommarin, 1987).

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References

  • Afoke, N. Y. P., Byers, P. D. and Hutton, W. C. (1987). Contact pressures in the human hip joint. J. Bone Jt Surg., 69B, 536–541

    Google Scholar 

  • Akeson, W. H., Woo, S. L.-Y., Amiel, D., Coutts, R. D. and Daniel, D. (1973). The connective tissue response to immobility: biochemical changes in periarticular connective

    Google Scholar 

  • tissue of the immobilized rabbit knee. Clin. Orthop., 93, 356–362

    Google Scholar 

  • Armstrong, C. G., Lai, W. M. and Mow, V. C. (1984). An analysis of the unconfined compression of articular cartilage. J. Biomech. Eng., 106, 165–173

    Google Scholar 

  • Aydelotte, M. B., Schleyerbach, R., Zeck, B. J. and Kuettner, K. E. (1986). In Kuettner, K. E., Schleyerbach, R. and Hascall, V. C. (Eds), Articular Cartilage Biochemistry. Raven Press, New York, pp. 235–256

    Google Scholar 

  • Bayliss, M. T., Ridgway, G. D. and Ali, S. Y. (1984). Delayed aggregation of proteoglycans in adult human articular cartilage. Biosci. Rep., 4, 827–833

    Google Scholar 

  • Bayliss, M. T., Urban, J. P. G., Johnstone, B. and Holm, S. (1986). In vitro method for measuring synthesis rates in the intervertebral disc. J. Orthop. Res., 4, 10–17

    Google Scholar 

  • Benya, P. D. and Nimni, M. E. (1979). The stability of the collagen phenotype during stimulated collagen, glycosaminoglycan and DNA synthesis. Arch. Biochem. Biophys., 192, 327–335

    Google Scholar 

  • Benya, P. D. and Shaffer, J. D. (1982). Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell, 30, 215–224

    Google Scholar 

  • Broom, N. D. and Myers, D. B. (1980). A study of the structural response of wet hyaline cartilage to various loading situations. Conn. Tiss. Res., 7, 227–237

    Google Scholar 

  • Buckwalter, J., Hunziker, E., Rosenberg, L., Coutts, R., Adams, M. and Eyre, D. (1988). In Woo, S. L.-Y. and Buckwalter, J. A. (Eds), Injury and Repair of the Musculoskeletal Soft Tissue. American Academy of Orthopaedic Surgeons, pp. 405–425

    Google Scholar 

  • Buschmann, M. D., Gluzband, Y. A., Grodzinsky, A. J., King, K. B., Kimura, J. H. and Kuettner, K. E. (1989). Chondrocytes in agarose gel synthesize a mechanically functional matrix. Trans. Orthop. Res. Soc, 15, 86

    Google Scholar 

  • Buschmann, M. D., Grodzinsky, A. J. and Kimura, J. H. (1988). Electromechanical detection of chondrocyte biosynthesis: in vitro model for electrical and mechanical stimulation of cartilage cells. Trans. BRAGS, 8, 1

    Google Scholar 

  • Caterson, B. and Lowther, D. A. (1978). Changes in the metabolism of the proteoglycans from sheep articular cartilage in response to mechanical stress. Biochim. Biophys. Acta, 540, 412–422

    Google Scholar 

  • Chun, L. E., Koob, T. J. and Eyre, D. R. (1986). Sequential enzymic dissection of the proteoglycan complex from articular cartilage. Trans. Orthop. Res. Soc, 11, 96

    Google Scholar 

  • Cleland, R. L., Wang, J. L. and Detweiler, D. M. (1982). Polyelectrolyte properties of sodium hyaluronate: potentiometric titration of hyaluronic acid. Macromolecules, 15, 386

    Google Scholar 

  • Eisenberg, S. R. and Grodzinsky, A. J. (1985). Swelling of articular cartilage and other connective tissues: electromechanochemical forces. J. Orthop. Res., 3, 148–159

    Google Scholar 

  • Eisenberg, S. R. and Grodzinsky, A. J. (1987). The kinetics of chemically induced nonequilibrium swelling of articular cartilage and corneal stroma. J. Biomech. Eng., 109, 79–89

    Google Scholar 

  • Flory, P. J. (1953). Principles of Polymer Chemistry. Cornell University Press, Ithaca, New York

    Google Scholar 

  • Frank, E. H. (1987). Electromechanics of Normal and Degenerated Cartilage: Poroelastic Behavior and Electrokinetic Mechanisms. Ph.D. thesis, MIT

    Google Scholar 

  • Frank, E. H. and Grodzinsky, A. J. (1987a). Cartilage electromechanics—I. Electrokinetic transduction and the effects of electrolyte pH and ionic strength. J. Biomech., 20, 615–627

    Google Scholar 

  • Frank, E. H. and Grodzinsky, A. J. (1987b). Cartilage electromechanics—II. A continuum model of cartilage electrokinetics and correlation with experiments. J. Biomech., 20, 629–639

    Google Scholar 

  • Frank, E. H. and Grodzinsky, A. J. (1989). Multi-layer model of cartilage electromechanics (in preparation)

    Google Scholar 

  • Frank, E. H., Grodzinsky, A. J., Kavesh, N. G. and Eisenberg, S. R. (1985). In Langrana, N. A. (Ed.), 1985 Advances in Bioengineering. ASME, New York, pp. 5–6

    Google Scholar 

  • Frank, E. H., Grodzinsky, A. J., Koob, T. J. and Eyre, D. R. (1987). Streaming potentials: a sensitive index of enzymatic degradation in articular cartilage. J. Orthop. Res., 5, 497–508

    Google Scholar 

  • Fukada, E. et al. (Eds) (1985). Bioelectrical Repair and Growth. Nishimura, Asahimachi-dori, Niigata, Japan

    Google Scholar 

  • Gillard, G. C., Reilly, H. C., Bell-Booth, P. G. and Flint, M. H. (1979). The influence of mechanical forces on the glycosaminoglycan content of the rabbit flexor digitorium profundus tendon. Conn. Tiss. Res., 7, 37

    Google Scholar 

  • Gray, M. L., Pizzanelli, A. M., Grodzinsky, A. J. and Lee, R. C. (1988). Mechanical and physicochemical determinants of the chondrocyte biosynthetic response. J. Orthop. Res., 6, 777–792

    Google Scholar 

  • Gray, M. L., Pizzanelli, A. M., Lee, R. C., Grodzinsky, A. J. and Swann, D. A. (1989). Kinetics of the chondrocyte biosynthetic response to compressive load and release. Biochim. Biophys. Acta, 991, 415–425

    Google Scholar 

  • Gritzka, T. L., Fry, L. R., Cheesman, R. L. and Lavigne, A. (1973). Deterioration of articular cartilage caused by continuous compression in a moving rabbit joint. J. Bone Jt Surg., 55A, 1698–1720

    Google Scholar 

  • Grodzinsky, A. J. (1983). Electromechanical and physicochemical properties of connective tissue. CRC Crit. Rev. Bioeng., 9, 133–199

    Google Scholar 

  • Grodzinsky, A. J., Roth, V., Myers, E., Grossman, W. D. and Mow, V. C. (1981). The significance of electromechanical and osmotic forces in the nonequilibrium swelling behavior of articular cartilage in tension. J. Biomech. Eng., 103, 221–231

    Google Scholar 

  • Hardingham, T. E., Fitton-Jackson, S. and Muir, H. (1972). Replacement of proteoglycans in embryonic chick cartilage in organ culture after treatment with testicular hyaluronidase. Biochem. J., 129, 101–112

    Google Scholar 

  • Hascall, V. C., Handley, C. J., McQuillan, D. J., Hascall, G. K., Robinson, H. C. and Lowther, D. A. (1983). The effect of serum on biosynthesis of proteoglycans by bovine articular cartilage in culture. Arch. Biochem. Biophys., 224, 206–223

    Google Scholar 

  • Hay, E. D. (Ed.) (1981). Cell Biology of Extracellular Matrix. Plenum Press, New York Heinegård, D. and Paulsson, M. (1987). Methods in Enzymology, 145, 336–363

    Google Scholar 

  • Heinegård, D. and Sommarin, Y. (1987). Methods in Enzymology, 144, 305–319

    Google Scholar 

  • Hodge, W. A., Fijan, R. S., Carlson, K. L., Burgess, R. G., Harris, W. H. and Mann, R. W. (1986). Contact pressures in the human hip joint measured in vivo. Proc. Natl Acad. Sci. USA, 83, 2879–2883

    Google Scholar 

  • Holmes, M. H., Lai, W. M. and Mow, V. C. (1985). Singular perturbation analysis of the nonlinear, flow-dependent compressive stress relaxation behavior of articular cartilage. J. Biomech. Eng., 107, 206–218

    Google Scholar 

  • Huberti, H. H. and Hayes, W. C. (1984). Patellofemoral contact pressures: the influence of q-angle and tendofemoral contact. J. Bone Jt Surg., 66A, 715–724

    Google Scholar 

  • Hunziker, E. R. and Schenk, R. K. (1987). In Wight, T. N. and Mecham, R. P. (Eds), Biology of Proteoglycans. Academic Press, Orlando, pp. 155–186

    Google Scholar 

  • Jones, I. L., Klamfeldt, D. D. S. and Sandstrom, T. (1982). The effect of continuous mechanical pressure upon the turnover of articular cartilage proteoglycans in vitro. Clin. Orthop., 165, 283–289

    Google Scholar 

  • Kempson, G. E. (1980). The mechanical properties of articular cartilage. In Sokoloff, L. (Ed.), The Joints and Synovial Fluid. Academic Press, New York, pp. 140–176

    Google Scholar 

  • Kiviranta, I., Jurvelin, J., Tammi, M., Saamanen, A.-M. and Helminen, H. J. (1987). Weight bearing controls glycosaminoglycan concentration and articular cartilage thickness in the knee joints of young beagle dogs. Arthr. Rheum., 30, 801–809

    Google Scholar 

  • Koob, T. J. and Vogel, K. G. (1987). Proteoglycan synthesis in organ cultures from regions of bovine tendon subjected to different mechanical forces. Biochem. J., 246, 589–598

    Google Scholar 

  • Kuettner, K. E. and Lindenbaum, A. (1965). Analysis of mucopolysaccharides in partially aqueous media. Biochim. Biophys. Acta, 101, 223

    Google Scholar 

  • Kuettner, K., Schleyerbach, R. and Hascall, V. C. (Eds) (1986). Articular Cartilage Biochemistry. Raven Press, New York

    Google Scholar 

  • McDevitt, C. A., Gilbertson, E. and Muir, H. (1977). An experimental model of osteoarthritis, early morphological and biochemical changes. J. Bone Jt Surg., 59B, 24–35

    Google Scholar 

  • McQuillan, D. J., Handley, C. J., Campbell, M. A., Bolis, S., Milway, V. E. and Herington, A. C. (1986). Stimulation of proteoglycan biosynthesis by serum and insulin-like growth factor-I in cultured bovine articular cartilage. Biochem. J., 240, 423–430

    Google Scholar 

  • Mak, A. F. (1986). Unconfined compression of hydrated viscoelastic tissues: a biphasic poroviscoelastic analysis. Biorheology, 23, 371–383

    Google Scholar 

  • Maroudas, A. (1976). Transport of solutes through articular cartilage. J. Anat., 122, 335–347

    Google Scholar 

  • Maroudas, A. (1979). In Freeman, M. A. R. (Ed.), Adult Articular Cartilage, 2nd edn. Pitman Medical, Tunbridge Wells, pp. 215–290

    Google Scholar 

  • Maroudas, A. (1980). In Sokoloff, L. (Ed.), The Joints and Synovial Fluid, Vol. 2. Academic Press, New York, pp. 239–291

    Google Scholar 

  • Maroudas, A., Ziv, I., Weisman, N. and Venn, M. (1985). Studies of hydration and swelling pressure in normal and osteoarthritic cartilage. Biorheology, 22, 159–169

    Google Scholar 

  • Matthews, M. B. (1959). Macromolecular properties of isomeric chondroitin sulfates. Biochim. Biophys. Acta, 35, 9

    Google Scholar 

  • Mecham, R. P. (Ed.) (1986). Regulation of Matrix Accumulation. Academic Press, Orlando Melching, L. I. and Roughley, R. J. (1985). The role of link protein in mediating the interaction between hyaluronic acid and newly secreted proteoglycan subunits from adult human articular cartilage. J. Biol. Chem., 260, 16279–16285

    Google Scholar 

  • Morgenthaler, A. W. (1987). Electromechanical Response of Articular Cartilage Subject to Enzymatic Degradation. Master’s Thesis, MIT

    Google Scholar 

  • Mow, V. C., Holmes, M. H. and Lai, W. M. (1984). Fluid transport and mechanical properties of articular cartilage: a review. J. Biomech., 17, 377–394

    Google Scholar 

  • Mow, V. and Rosenwasser, M. (1988). In Woo, S. L.-Y. and Buckwalter, J. A. (Eds), Injury and Repair of the Musculoskeletal Soft Tissue. American Academy of Orthopaedic Surgeons, pp. 427–463

    Google Scholar 

  • Muir, I. H. M. (1980). In Sokoloff, L. (Ed.), The Joints and Synovial Fluid. Academic Press, New York, pp. 28–94

    Google Scholar 

  • Myers, E. R., Lai, W. M. and Mow, V. C. (1984). A continuum theory and an experiment for the ion-induced swelling behavior of articular cartilage. J. Biomech. Eng., 106, 151–158

    Google Scholar 

  • Oegema, T. R. (1980). Delayed formation of proteoglycan aggregate structures in human articular cartilage disease states. Nature, 288, 583–585

    Google Scholar 

  • Olah, E. H. and Kostenszky, K. S. (1972). Effect of altered functional demand on the glycosaminoglycan content of the articular cartilage of dogs. Acta Biol. Hung., 23, 195–200

    Google Scholar 

  • Overbeek, J. T. (1952). In Kruyt, H. R. (Ed.), Colloid Science I. American Elsevier, New York, pp. 115–193

    Google Scholar 

  • Palmoski, M. J. and Brandt, K. D. (1984). Effects of static and cyclic compressive loading on articular cartilage plugs in vitro. Arthr. Rheum., 27, 675–681

    Google Scholar 

  • Palmoski, M. J., Perricone, E. and Brandt, K. D. (1979). Development and reversal of a proteoglycan aggregation defect in normal canine knee cartilage after immobilization. Arthr. Rheum., 22, 508–517

    Google Scholar 

  • Phillips, S. L. (1984). The Determination of Charge Density in Articular Cartilage via Chemical Titration. Master’s Thesis, MIT

    Google Scholar 

  • Plaas, A. J. K. and Sandy, J. D. (1986). The affinity of newly synthesized proteoglycan for hyaluronic acid can be enhanced by exposure to mild alkali. Biochem. J., 234, 221–223

    Google Scholar 

  • Poole, C. A., Flint, M. H. and Beaumont, B. W. (1984). Morphological and functional interrelationships of articular cartilage matrices. J. Anat., 138, 113–138

    Google Scholar 

  • Radin, E. L., Martin, R. B., Burr, D. B., Caterson, B., Boyd, R. D. and Goodwin, C. (1984). Effects of mechanical loading on the tissues of the rabbit knee. J. Orthop. Res., 2, 221–234

    Google Scholar 

  • Sachs, J. R. (1987). A Mathematical Model of an Electromechanically Coupled Poroelastic Medium. Ph.D. Thesis, MIT

    Google Scholar 

  • Sachs, J. R. and Grodzinsky, A. J. (1989). An electromechanically coupled poroelastic medium driven by an applied electric current: surface detection of bulk material properties. Physiochem. Hydrodynam., 11, 585–614

    Google Scholar 

  • Sah, R. L., Doong, J. Y. H., Kim, Y. J., Grodzinsky, A. J., Plaas, A. H. K. and Sandy, J. D. (1988). Biosynthetic response of cartilage expiants to mechanical and physicochemical stimuli. Trans. Orthop. Res. Soc., 13, 70

    Google Scholar 

  • Sah, R. L., Doong, J. Y. H., Tiao, P. M., Grodzinsky, A. J., Plaas, A. H. K. and Sandy, J. D. (1989b). Catabolic response of cartilage expiants to mechanical stimuli. Trans. Orthop. Res. Soc., 14, 50

    Google Scholar 

  • Sah, R. L.-Y., Grodzinsky, A. J., Plaas, A. H. K. and Sandy, J. D. (1989a). Effects of tissue compression on the hyaluronate binding properties of newly synthesized proteoglycans in cartilage expiants. Biochem. J. (in press)

    Google Scholar 

  • Sah, R. L., Kim, Y. J., Doong, J. Y. H., Grodzinsky, A. J., Plaas, A. H. K. and Sandy, J. D. (1989c). Biosynthetic response of cartilage expiant to dynamic compression. J. Orthop. Res., 7, 619–636

    Google Scholar 

  • Schneiderman, R., Keret, D. and Maroudas, A. (1986). Effects of mechanical and osmotic pressure on the rate of glycosaminoglycan synthesis in the human adult femoral head cartilage: an in vitro study. J. Orthop. Res., 4, 393–408

    Google Scholar 

  • Sun, D., Aydelotte, M. B., Maldonado, B., Kuettner, K. E. and Kimura, J. H. (1986).

    Google Scholar 

  • Clonal analysis of the population of chondrocytes from the swarm rat chondrosarcoma in agarose culture. J. Orthop. Res., 4, 427–436

    Google Scholar 

  • Sweet, M. B. E., Thonar, E. J. M. A. and Immelman, A. R. (1977). Regional distribution of water and glycosaminoglycan in immature articular cartilage. Biochim. Biophys. Acta, 500, 173–186

    Google Scholar 

  • Urban, J. P. G., Maroudas, A., Bayliss, M. T. and Dillon, J. (1979). Swelling pressures of proteoglycans at the concentrations found in cartilaginous tissues. Biorheology, 16, 447–464

    Google Scholar 

  • Vasan, N. (1983). Effects of physical stress on the synthesis and degradation of cartilage matrix. Conn. Tiss. Res., 12, 49–58

    Google Scholar 

  • Veis, A. (1967). In Ramachandran, G. N. (Ed.), Treatise on Collagen. Academic Press, Orlando, p. 367127–166

    Google Scholar 

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Authors
  1. Alan J. Grodzinsky
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  2. Eliot H. Frank
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Editors and Affiliations

  1. Department of Medical Biophysics, University of Manchester, UK

    David W. L. Hukins

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© 1990 The Macmillan Press Ltd

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Grodzinsky, A.J., Frank, E.H. (1990). Electromechanical and Physicochemical Regulation of Cartilage Strength and Metabolism. In: Hukins, D.W.L. (eds) Connective Tissue Matrix. Topics in Molecular and Structural Biology. Palgrave, London. https://doi.org/10.1007/978-1-349-09865-1_5

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  • DOI: https://doi.org/10.1007/978-1-349-09865-1_5

  • Publisher Name: Palgrave, London

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  • Online ISBN: 978-1-349-09865-1

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