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Monoclonal Antibodies Directed Against Human Immunoglobulins: Preparation and Evaluation Procedures

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Reviews on Immunoassay Technology

Abstract

Monoclonal antibodies (Mabs) are prepared by immunizing an animal (generally a mouse or rat) and fusing the spleen cells, after a given period of time, with a plasmacytoma cell line (Köhler and Milstein, 1975). The fusion mixture is plated out into such numbers of microcultures that the likelihood of obtaining cultures with single fusion events is reasonable (De Blas et al., 1981). Supernatants from individual cultures are tested with a suitable immunoassay for the presence of antibodies with the desired specificity.

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Authors
  1. J. J. Haaijman
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  2. J. Coolen
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  3. C. Deen
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  4. C. J. M. Kröse
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  5. J. J. Zijlstra
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  6. J. Radl
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Editors and Affiliations

  1. Abteilung für Innere Medizin I, Universität Ulm, 7900 Ulm, FR Germany

    S. B. Pal

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© 1988 S. B. Pal and the Contributors

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Haaijman, J.J., Coolen, J., Deen, C., Kröse, C.J.M., Zijlstra, J.J., Radl, J. (1988). Monoclonal Antibodies Directed Against Human Immunoglobulins: Preparation and Evaluation Procedures. In: Pal, S.B. (eds) Reviews on Immunoassay Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-09854-5_3

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