Skip to main content

Chemiluminescence — An Ultrasensitive Analytical Tool in Medicine and Biotechnology

  • Chapter
Resources and Applications of Biotechnology
  • 332 Accesses

Abstract

Walk out in one of the quiet Welsh valleys on a warm summer’s night and you might be lucky enough to see the bright green glow of a female beetle. This is the European glow-worm (Figure 1) trying to attract a mate. Such luminous phenomena are rare on land, but are much more common in the sea. In fact, animal light plays a major role in the ecology of the deep oceans, where as many as nine out of ten of all the fish may be luminous7. Here we find the extraordinary angler fish with its luminous lure containing a culture of bacteria, and the remarkable Malacosteus with its two pairs of light organs, one emitting blue, the other red, light (Figure 2). There are also many invertebrate examples of living light, including starfish, shrimps, tunicates and jellyfish. The sources of this light are chemical reactions within specialised cells in the animal.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 69.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. Boveris, A., Cadenas, E. and Chance, B. (1981). Ultraweak chemiluminescence: A sensitive assay for oxidated radical reactions. Fed. Proc., 40, 195–198

    Google Scholar 

  2. Campbell, A. K. (1986). Living light: chemiluminescence in the research and clinical laboratory. TIBS, 11, 104–108

    Google Scholar 

  3. Campbell, A. K. (1987). Intracellular calcium: friend or foe? Clinical Science, 72

    Google Scholar 

  4. Campbell, A. K., Hallett, M. B. and Weeks, I. (1985). Chemiluminescence as an analytical tool in cell biology and medicine. Methods of Biochemical Analysis, 31, 317–416

    Article  Google Scholar 

  5. Campbell, A. K. and Herring, C. J. (1986). A novel red fluorescent protein from the deep sea luminous fish Malacosteus niger. Comparative Biochemistry and Physiology

    Google Scholar 

  6. Campbell, A. K., Roberts, P. A. and Patel, A. (1985). Chemiluminescence energy transfer: a technique for homogeneous immunoassay. In Collins, W. P. (Ed.), Alternative Immunoassays, pp. 153–183. John Wiley, Chichester

    Google Scholar 

  7. Herring, P. J. (Ed.) (1978). Bioluminescence in Action. Academic Press, London

    Google Scholar 

  8. Matthews, J. A., Batki, A., Hynds, C. and Kricka, L. J. (1985). Enhanced chemiluminescence method for the detection of DNA dot-hybridization assays. Analytical Biochemistry, 151, 205–209

    Article  Google Scholar 

  9. Roberts, C. A. and Campbell, A. K. (1987). Pholasin — a bioluminescent indicator for detecting activation of single neutrophils. Analytical Biochemistry

    Google Scholar 

  10. Thorpe, G. H., Kricka, L. J., Gillespie, E., Moseley, S., Amess, R., Baggett, N. and Whitehead, T. P. (1985). Enhancement of the horseradish peroxidase-catalysed chemiluminescent oxidation of cyclic diacylhydrazides by 6-hydroxybenzothiazoles. Clinical Chemistry, 31, 1335–1341

    Google Scholar 

  11. Weeks, I., Beleshti, I., McCapra, F., Campbell, A. K. and Woodhead, J. S. (1983). Acridinium esters as high-specific activity label in immunoassay. Clinical Chemistry, 29, 1474–1479

    Google Scholar 

  12. Whitehead, T. C., Thorpe, G. H. G., Carter, T. J. N., Groucutt, C. and Kricka, L. J. (1983). Enhanced luminescence procedure for sensitive determination of peroxidase-label conjugates in immunoassay. Nature, Lond., 305, 158–159

    Article  Google Scholar 

  13. Williams, E. J. and Campbell, A. K. (1986). A homogeneous assay for biotin based on chemiluminescence energy transfer. Analytical Biochemistry, 155, 249–255

    Article  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Copyright information

© 1988 The Editor and the Contributors

About this chapter

Cite this chapter

Campbell, A.K. (1988). Chemiluminescence — An Ultrasensitive Analytical Tool in Medicine and Biotechnology. In: Greenshields, R. (eds) Resources and Applications of Biotechnology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-09574-2_9

Download citation

Publish with us

Policies and ethics