Abstract
Dopamine (DA) receptors have been identified in various areas of the mammalian central nervous system (CNS) by conventional in vitro binding techniques using radiolabelled DA agonists and antagonists (see Seeman, 1980 for a review). However, in order to better understand the organization of DA-containing systems within the CNS, DA receptors need to be localized more precisely within the various DA-rich areas. The recent development of light microscopic receptor autoradiography (Young and Kuhar, 1979; Murrin, 1981; Palacios, 1981) has provided a means to analyze the localization of specific sites quantitatively and with a high degree of anatomical resolution in the CNS. In the present study we have used the technique of autoradiography to investigate in detail the anatomical distribution of DA receptors in the rat brain and spinal cord subregions and in selected regions of the human brain using 3H-N-propyl- norapomorphine, a potent DA receptor agonist, as a ligand.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Biegon, A. and Rainbow, T.C. (1983). Localization and characterization of 3H-desmethylimipramine binding sites in rat brain by quantitative autoradiography. J. Neurosci. 3, 1069–1076.
Björklund, A. and Skagerberg, G. (1979). Evidence for a major spinal cord projection from the diencephalic A11 dopamine cell group in the rat using transmitter specific fluorescent retrograde tracing. Brain Res. 177, 170–175.
Bokobza, B., Ruberg, M., Scatton, B., Javoy-Agid, F. and Agid, Y. (1982). 3H-spiperone binding, dopamine and HVA concentrations in Parkinson’s disease and supranuclear palsy. Europ. J. Pharmacol. 99, 167–175.
Commissiong, J.W., Gentleman, S. and Neff, N.H. (1979). Spinal cord dopaminergic neurons: evidence for an uncrossed nigrospinal pathway Neuropharmacology 18, 565–568.
Commissiong, J.W. and Neff, N.H. (1979). Current status of dopamine in the mammalian spinal cord. Biochem. Pharmacol. 28, 1569–1573.
Demenge, P., Mouchet, P., Guerin, B. and Feuerstein, C. (1981). Identification and distribution of neuroleptic binding sites in the rat spinal cord. J. Neurochem. 37, 53–59.
Dubocovich, M.L. and Zahniser, N.R. (1983). Binding of the dopamine uptake inhibitor 3H-nomifensine to striatal membranes. Soc. Neurosci. Abstr. 9 564.
Fuxe, K., Calza, L., Benfenati, F., Zini, I. and Agnati, L.F. (1983). Quantitative autoradiographic localization of 3H-imipramine binding sites in the brain of the rat: relationship to ascending 5-hydroxy-tryptamine neuron systems. Proc. Natl. Acad. Sci. USA, 80, 3836–3840.
Hall, M.D., Jenner, P., Kelly, E. and Marsden, C.D. (1983). Differential anatomical location of 3H-N, n-propylnorapomorphine and 3H- spiperone binding sites in the striatum and substantia nigra of the rat. Brit. J. Pharmacol. 79, 599–610.
Hunt, P., Kannengiesser, M.H. and Raynaud, J.P. (1974). Nomifensine: a new potent inhibitor of dopamine uptake into synaptosomes from rat brain corpus striatum. J. Pharm. Pharmac. 26, 370–371.
Javitch, J.A., Blaustein, R.O. and Snyder, S.H. (1983). 3H-mazindol binding associated with neuronal dopamine uptake sites in corpus striatum membranes. Europ. J. Pharmacol. 90, 461–462.
Jones, B.E. and Moore, R.Y. (1977). Ascending projections of the locus coeruleus in the rat. II. Autoradiographic studies. Brain Res. 127, 23–53.
Kennedy, L.T. and Hanbauer, I. (1983). Sodium-sensitive cocaine binding to rat striatal membrane: possible relationship to dopamine uptake sites. J. Neurochem. 41, 172–178.
Langer, S.Z., Moret, C., Raisman, R., Dubocovich, M.L. and Briley, M. (1980). High affinity 3H-imipramine binding in rat hypothalamus is associated with the uptake of serotonin but not norepinephrine, Science 210, 1133–1135.
Lee, C.M. and Snyder, S.H. (1981). Norepinephrine neuronal uptake binding sites in rat brain membranes labelled with 3H-desipramine. Proc. Natl. Acad. Sci. USA 78, 5250–5254.
Lindvall, O. and Bjorklund, A. (1978). Anatomy of the dopaminergic neuron system in the rat brain. In Dopamine Advances in Biochemical Psychopharmacology, Vol. 19 (eds P.J. Roberts, G.N. Woodruff and L. L. Iversen). Raven Press, New York, pp 1–23.
Murrin, C.L. (1981). Neurotransmitter receptors: neuroanatomical localization through autoradiography. Int. Review of Neurobiol. 22, 111–171.
Palacios, J., Niehoff, D., Kuhar, M. (1981). Receptor autoradiography with tritium-sensitive film. Potential for computerized densitometry. Neurosci. Lett. 25, 101–105.
Pimoule, C., Schoemaker, H., Javoy-Agid, F., Scatton, B., Agid, Y. and Langer, S.Z. (1983). Decrease in 3H-cocaine binding to the dopamine transporter in Parkinson’s disease. Europ. J. Pharmacol. 95, 145–146.
Raisman, R., Briley, M.S. and Langer, S.Z. (1980). Specific tricyclic antidepressant binding sites in rat brain characterized by high affinity 3H-imipramine binding. Europ. J. Pharmacol. 61, 373–380.
Scatton, B., Dubois, A. and Cudennec, A. (1984). Autoradiographic localization of dopamine receptors in the spinal cord of the rat using 3H-N-propylnorapomorphine. J. Neural Transm. 59, in press.
Scatton, B., Simon, H., Le Moal, M. and Bischoff, S. (1980). Origin of dopaminergic innervation of the rat hippocampal formation. Neurosci. Lett. 18, 125–131.
Schoemaker, H., Pimoule, C., Arbilla, S., Scatton, B., Javoy-Agid, F., Agid, Y. and Langer, S.Z. (1984). Sodium dependent 3H-cocaine binding associated with dopamine uptake sites parallels dopaminergic denervation in Parkinson’s disease. Submitted.
Seeman, P. (1980). Brain dopamine receptors. Pharmacol. Reviews 32, 229–313.
Steele, J.C., Richardson, J.C. and Olzewski, J. (1964). Progressive supranuclear palsy. A heterogeneous degeneration involving the brain stem, basal ganglia and cerebellum with vertical gaze and pseudobulbar palsy, nuchal dystonia and dementia. Arch. Neurol. 10, 333–343.
Unnerstall, J.R., Niehoff, D.L., Kuhar, M.J. and Palacios, J.M. (1982). Quantitative receptor autoradiography using 3H-ultrofilm: application to multiple benzodiazepine receptors. J. Neurosci. Meth. 6, 59–73.
Versteeg, D.H., Van der Gugten, J., De Jong, Z. and Palkovits, M. (1976). Regional concentrations of noradrenaline and dopamine in rat brain. Brain Res. 113, 563–574.
Young, W.S. and Kuhar, M.J. (1979). A new method for receptor autoradiography: 3H-opioid receptor labelling in mounted tissue sections. Brain Res. 179, 255–270.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Copyright information
© 1985 The Wenner-Gren Centre
About this chapter
Cite this chapter
Scatton, B. et al. (1985). Quantitative Autoradiographic Localization of Dopamine Receptors and Uptake Sites in the Rat and Human Cns. In: Agnati, L.F., Fuxe, K. (eds) Quantitative Neuroanatomy in Transmitter Research. Wenner-Gren Center International Symposium Series. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-08171-4_23
Download citation
DOI: https://doi.org/10.1007/978-1-349-08171-4_23
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-1-349-08173-8
Online ISBN: 978-1-349-08171-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)