Quantitative Autoradiographic Localization of Dopamine Receptors and Uptake Sites in the Rat and Human Cns
Dopamine (DA) receptors have been identified in various areas of the mammalian central nervous system (CNS) by conventional in vitro binding techniques using radiolabelled DA agonists and antagonists (see Seeman, 1980 for a review). However, in order to better understand the organization of DA-containing systems within the CNS, DA receptors need to be localized more precisely within the various DA-rich areas. The recent development of light microscopic receptor autoradiography (Young and Kuhar, 1979; Murrin, 1981; Palacios, 1981) has provided a means to analyze the localization of specific sites quantitatively and with a high degree of anatomical resolution in the CNS. In the present study we have used the technique of autoradiography to investigate in detail the anatomical distribution of DA receptors in the rat brain and spinal cord subregions and in selected regions of the human brain using 3H-N-propyl- norapomorphine, a potent DA receptor agonist, as a ligand.
KeywordsDopamine Dementia Serotonin Gelatin Levodopa
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- Dubocovich, M.L. and Zahniser, N.R. (1983). Binding of the dopamine uptake inhibitor 3H-nomifensine to striatal membranes. Soc. Neurosci. Abstr. 9 564.Google Scholar
- Fuxe, K., Calza, L., Benfenati, F., Zini, I. and Agnati, L.F. (1983). Quantitative autoradiographic localization of 3H-imipramine binding sites in the brain of the rat: relationship to ascending 5-hydroxy-tryptamine neuron systems. Proc. Natl. Acad. Sci. USA, 80, 3836–3840.PubMedCentralCrossRefPubMedGoogle Scholar
- Lindvall, O. and Bjorklund, A. (1978). Anatomy of the dopaminergic neuron system in the rat brain. In Dopamine Advances in Biochemical Psychopharmacology, Vol. 19 (eds P.J. Roberts, G.N. Woodruff and L. L. Iversen). Raven Press, New York, pp 1–23.Google Scholar
- Scatton, B., Dubois, A. and Cudennec, A. (1984). Autoradiographic localization of dopamine receptors in the spinal cord of the rat using 3H-N-propylnorapomorphine. J. Neural Transm. 59, in press.Google Scholar
- Schoemaker, H., Pimoule, C., Arbilla, S., Scatton, B., Javoy-Agid, F., Agid, Y. and Langer, S.Z. (1984). Sodium dependent 3H-cocaine binding associated with dopamine uptake sites parallels dopaminergic denervation in Parkinson’s disease. Submitted.Google Scholar
- Seeman, P. (1980). Brain dopamine receptors. Pharmacol. Reviews 32, 229–313.Google Scholar