Quantitative Autoradiographic Localization of Dopamine Receptors and Uptake Sites in the Rat and Human Cns
Dopamine (DA) receptors have been identified in various areas of the mammalian central nervous system (CNS) by conventional in vitro binding techniques using radiolabelled DA agonists and antagonists (see Seeman, 1980 for a review). However, in order to better understand the organization of DA-containing systems within the CNS, DA receptors need to be localized more precisely within the various DA-rich areas. The recent development of light microscopic receptor autoradiography (Young and Kuhar, 1979; Murrin, 1981; Palacios, 1981) has provided a means to analyze the localization of specific sites quantitatively and with a high degree of anatomical resolution in the CNS. In the present study we have used the technique of autoradiography to investigate in detail the anatomical distribution of DA receptors in the rat brain and spinal cord subregions and in selected regions of the human brain using 3H-N-propyl- norapomorphine, a potent DA receptor agonist, as a ligand.
KeywordsSpinal Cord Dopamine Receptor Caudate Nucleus Human Central Nervous System Olfactory Tubercle
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