Abstract
Examples are presented on the use of computer-assisted image analysis for the quantitation of nerve terminal density of certain transmitter-identified (noradrenaline, serotonin, substance P) neuron systems in CNS and PNS demonstrated by histochemical and immunocytochemical techniques. The effects of selective neurotoxic lesions on these neurotransmitter systems and their behavior during conditions of regrowth was investigated. The results obtained with image analysis were compared with measurements of relevant neurochemical parameters for the respective neuron types under identical experimental conditions. The results from the neurochemical analyses were in general in good agreement with those obtained by quantitative image analysis. It is concluded that image analysis is a very powerful and useful tool for quantitation of transmitter-identified nerve terminal networks under conditions of dynamic changes (degen- eration-regrowth) in terminal density.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Agnati, L.F., Fuxe, K., Calza, L., Benefenati, F., Cavicchioli, L., Toffano, G. and Goldstein, M. (1983). Gangliosides increase the survival of lesioned nigral dopamine neurons and favour the recovery of dopaminergic synaptic function in striatum of rats by collateral sprouting. Acta Physiol. Scand. 119, 347–363.
Coons, A.H. (1958). Fluorescent antibody methods. In General Cyto- chemical Methods, Vol. I. (ed. J.F. Canielli). Academic Press, New York.
Corrodi, K. and Jonsson, G. (1967). The formaldehyde fluorescence method for the histochemical demonstration of biogenic monoamines. A review on the methodology. J. Histochem. Cytochem. 15, 65–78.
Cuello, A.C., Galfre, G. and Milstein, C. (1979). Detection of substance P in the central nervous system by a monoclonal antibody. Proc. Natl. Acad. Sci. 76, 3532–3536.
Falck, B., Hillarp, N.-Å., Thieme, G. and Torp, A. (1962). Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 10, 348–354.
Fuxe, K., Hökfelt, T., Jonsson, G. and Ungerstedt, U. (1970). Fluorescence microscopy in neuroanatomy. In Contemporary Research in Neuroanatomy, (eds. W.J.K. Nauta and S.O.E. Ebbesson). Springer-Verlag, Berlin.
Gorio, A., Marini, P. and Zanoni, R. (1983). Muscle reinnervation. III. Motoneuron sprouting capacity, enhancement by exogenous gangli- osides. Neuroscience 8, 417–429.
Janscó, G., Hökfelt, T., Lundberg, J.M., Kiraly, E, Halász, N., Nilsson, G., Terenius, L., Rehfeld, J., Steinbusch, H., Verhofstad, A., Elde, R., Said, S. and Brown, M. (1981). Immunohistochemical studies on the effect of capsaicin on spinal and medullary peptide and monoamine neurons using antisera to substance P, gastrin/CCK, VIP, enkephalin, neurotensin and 5-hydroxytryptamine. J. Neurocytol. 10, 963–980.
Jansco, G., Kiraly, E. and Jancsó-Gábor (1977). Pharmacologically induced selective degeneration of chemosensitive primary sensory neurons. Nature 270, 741–743.
Jonsson, G. (1981). Lesion methods in neurobiology. In Techniques in Neuroanatomical Research, (eds. Ch. Heym and W.G. Forssman). Springer-Verlag, Berlin.
Jonsson, G. (1983). Chemical lesioning techniques: Monoamine neurotoxins. In Handbook of Chemical Neuroanatomy. Vol. 1. Methods in Chemical Neuroanatomy, (eds. A. Björklund and T. Hökfelt). Elsevier, Amsterdam.
Jonsson, G., Gorio, A., Hallman, H., Janigro, D., Kojima, H. and Zanoni, R. (1984). Effect of GM1 ganglioside on neonatally neurotoxin induced degeneration of serotonin neurons in the rat brain. Develop. Brain Res. (in press).
Jonsson, G. and Hallman, H. (1982). Response of central monoamine neurons following an early neurotoxic lesion. Biblthca Anat. 23, 76–92.
Jonsson, G., Hallman, H., Pollase, T. and Sachs, Ch. (1978). Developmental plasticity of central serotonin neurons following 5,7- dihydroxytryptamine treatment. Ann. N.Y. Acad. Sci. 305, 328–345.
Jonsson, G., Hallman, H. and Sundstrom, E. (1982). Effects of the noradrenaline neurotoxin DSP4 on the postnatal development of central noradrenaline neurons in the rat. Neuroscience 7, 2895–2907.
Jonsson, G. and Sachs, Ch. (1970). Effects of 6-hydroxydopamine on the uptake and storage of noradrenaline in sympathetic adrenergic neurons. Eur. J. Pharmacol. 9, 141–155.
Jonsson, G. and Sachs, Ch. (1972). Neurochemical properties of adrenergic nerves regenerated after 6-hydroxydopamine. J. Neurochem. 19, 2577–2585.
Ljungdahl, Å., Hökfelt, T. and Nilsson, G. (1979). Distribution of substance P-like immunoreactivity in the central nervous system of the rat. I. Cell bodies and nerve terminals. Neuroscience 3, 861–943.
Malmfors, T. (1965). Studies on adrenergic nerves. Acta Physiol. Scand. 64 Suppl., 248.
Nagy, J.I., Vincent, S.R., Staines, W.M.A., Fibiger, H.C., Reisine, T.D. and Yamamura, H.I. (1980). Neurotoxic action of capsaicin on spinal substance P neurons. Brain Res. 186, 435–444.
Olson, L., Hamberger, B., Jonsson, G. and Malmfors, T. (1968). Combined fluorescence histochemistry and 3H-noradrenaline measurements of adrenergic nerves. Histochemie 15, 38–45.
Ponzio, F. and Jonsson, G. (1979). A rapid and simple method for the determination of picogram levels of serotonin in brain tissue using liquid chromatography with electrochemical detection. J. Neurosci. 32, 129–132.
Rapport, M.M. and Gorio, A. (eds.) (1981). Gangliosides in Neurological and Neuromuscular Function, Development and Repair. Raven Press, New York.
Sachs, Ch. and Jonsson, G. (1975). 5,7-Dihydroxytryptamine induced changes in the postnatal development of central 5-hydroxytryptamine neurons. Med. Biol. 53, 156–164.
Steinbush, H.W.M., Verhofstad, A.A.J. and Joosten, H.W. (1978). Localization of serotonin in the central nervous system by immuno- histochemistry: Description of a specific and sensitive technique and some applications. Neuroscience 3, 811–819.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Copyright information
© 1985 The Wenner-Gren Centre
About this chapter
Cite this chapter
Jonsson, G., Hallman, H., Luthman, J. (1985). Quantitation of Nerve Terminal Networks of Transmitter-Identified Neurons After Selective Neurotoxic Lesions. In: Agnati, L.F., Fuxe, K. (eds) Quantitative Neuroanatomy in Transmitter Research. Wenner-Gren Center International Symposium Series. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-08171-4_20
Download citation
DOI: https://doi.org/10.1007/978-1-349-08171-4_20
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-1-349-08173-8
Online ISBN: 978-1-349-08171-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)