The Mutagenicity of Benzidine, 4,4″-Diaminoterphenyl, N,N-Dimethyl-4-aminoazobenzene and 4-Cyano-N,N-dimethylaniline in the L51787 Mouse Lymphoma Assay
Four compounds were tested for their ability to cause mutation of L5178Y mouse lymphoma cells to 6-thioguanine resistance, as part of the Second UKEMS Collaborative Study. In these preliminary studies, the carcinogen benzidine was observed to have mutagenic activity in this assay in the presence but not the absence of liver S9. The benzidine analogue diaminoterphenyl was inactive. Dimethylaminoazo-benzene was also inactive, but this may have resulted from the use of an inappropriate metabolising system. Cyanodimethylaniline, a compound structurally related to dimethylaminoazobenzene, gave an equivocal result in the presence of S9, in the absence of S9 activation no activity was observed.
KeywordsMutation Rate Mutagenic Activity Mutation Assay L5178Y Cell Electronic Cell Counter
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