Abstract
The human lymphoblast line TK6, heterozygous at the thymidine kinase locus, has been developed for use as a mutation assay by Thilly and co-workers (Thilly et al., 1980; Liber and Tilley, 1982; Deluca et al., 1983 and Goldmacher et al., 1983). The cells grow in suspension culture and may be readily cloned with high efficiency using the microtitration method. We have selected for resistance to 6-thioguanine, trifluorothymidine and ouabain to test the mutagenic response of Tk6 cells following treatment with benzidine (BZD) and 4,4″-diaminotherphenyl (DAT). Experiments were carried out with and without Aroclor-induced rat S9 liver supernatant.
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Cole, J., Arlett, CF., Lowe, J. and Muriel, M.J. (1983). A comparison of the agar cloning and microtitration techniques for assaying cell survival and mutation frequency in L5178Y mouse lymphoma cells. Mutation Res. 111: 371–386
DeLuca, J.G., Weinstein, L. and Thilly, W.G. (1983). Ultraviolet light-induced mutation of diploid human lymphoblasts. Mutation Res. 107: 347–370
Goldmacher, V.S. and Thilly, W.G. (1983). Formaldehyde is mutagenic for cultured human cells. Mutation Res. 116: 417–422
Liber, H.L. and Thilly, W.G. (1982). Mutation assay at the thymidine kinase locus in diploid human lymphoblasts. Mutation Res. 94: 467–485
Thilly, W.G., DeLuca, J.G., Furth, E.E., Hoppe, H., Kaden, D.A., Krolewski, J.J., Liber, H.L., Skopek, T.R., Slapikoff, S.A., Tizard, R.J. and Penman, B.W. (1980). Gene-locus mutation assays in diploid human lymphoblast lines. In Chemical Mutagens, Vol.6, (eds. F.J. de Serres and A. Hollaender). Resolution: Global Plenum Pub. Corp. pp.331–364
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© 1985 United Kingdom Environmental Mutagen Society
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Cole, J., Arlett, C.F., Muriel, W.J. (1985). The Absence of Mutagenic Activity of Benzidine and 4,4″-Diaminoterphenyl in Tk6 Human Lymphoblast Cells. In: Parry, J.M., Arlett, C.F. (eds) Comparative Genetic Toxicology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-07901-8_35
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DOI: https://doi.org/10.1007/978-1-349-07901-8_35
Publisher Name: Palgrave Macmillan, London
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