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Gene Mutation in Yeast Induced by DAB, CDA, BZD and DAT

  • I. G. de Mitchell
  • P. J. Gilbert

Summary

DAB, CDA, BZD and DAT were tested for the induction of gene mutation in Saccharomyces cerevisiae strain XV185–14C 3. All these agents showed weak mutagenicity when tested in the presence of microsomes after the tester strain had been pretreated with nystatin to increase permeability. However, in the absence of microsomes or nystatin pretreatment, there was very little, or no, genetic activity at any of the loci under test.

Keywords

Yeast Nitrogen Base Cytochrome P450 Activity Forward Mutation Microsomal Activation Microbial Assay 
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Notes

  1. Ames, B.N., McCann, J., Yamasaki, E. (1975). Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutation Res. 31: 347–364PubMedCrossRefGoogle Scholar
  2. Ames, B.N., Durston, W.E, Yamasaki, E. and Lee F.D. (1973). Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. 70: 2281–2285PubMedPubMedCentralCrossRefGoogle Scholar
  3. Callen, D.F. and Philpot R.M. (1977). Cytochrome P-450 and the activation of promutagens in Saccharomyces cerevisiae. Mutation Res. 45: 309–324PubMedCrossRefGoogle Scholar
  4. Cleland, W.W. (1964). Dithiothreitol a new protective reagent for SH groups. Biochemistry 3: 480–482PubMedCrossRefGoogle Scholar
  5. Elion-Gerritzen, W.E. (1980). Analytical precision in clinical chemistry and medical decisions. Am. J. Clin. Pathol. 73: 183–195PubMedGoogle Scholar
  6. Garner, R.C., Miller, E.C. and Miller, J.A. (1972). Liver microsomal metabolism of Aflatoxin Bl to a reactive derivative toxic to Salmonella typhimurium TA1530. Cancer Res. 32: 2058–2066PubMedGoogle Scholar
  7. Kelly, D. and Parry, J.M. (1983). Metabolic activation of cytochrome P-450/P-448 in the yeast Saccharomyces cerevisiae. Mutation Res. 108: 147–159PubMedCrossRefGoogle Scholar
  8. Kotler-Brajtburg, J., Medoff, G., Kobayashi, G.S., Boggs, S., Schlessinger, D., Pandey, C. and Reinhart, K.L. (1979). Classification of polyene antibiotics according to structure and biological effects. Antimicrob. Agents and Chemother. 15: 716–722CrossRefGoogle Scholar
  9. Lieve, L. (1968). Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. J. Biol. Chem. 243: 2373–2380Google Scholar
  10. Mehta, R.D. and von Borstel, R.C. (1981). Mutagenic activity of 42 encoded compounds in the haploid yeast reversion assay, strain XV 185–14C. In Evaluation of Short-term Tests for Carcinogens (Progress in Mutation Research Ser. Vol.1) (eds. F.J. de Serres and J. Ashby). Elsevier/ North Holland, pp.414–423Google Scholar
  11. Mitchell, I. de G. (1974). A comparison of the sensitivity and specificity of microbial systems for assessing genetic damage. Agents and Actions 4: 286–294CrossRefGoogle Scholar
  12. Mitchell, I. de G. (1978). Microbial assays for mutagenicity: a modified liquid culture method compared with the agar plate system for precision and sensitivity. Mutation Res. 54: 1–16PubMedCrossRefGoogle Scholar
  13. Mitchell, I. de G., Dixon, P.A, Gilbert, P.J. and White, D.J. (1980). Mutagenicity of antibiotics in microbial assays; problems of evaluation. Mutation Res. 79: 91–105CrossRefGoogle Scholar
  14. Moustacchi, E. (1980). Mutagenicity testing with eukaryotic microorganisms. Arch. Toxicol. 46: 99–110PubMedCrossRefGoogle Scholar
  15. Wiseman, A. (1976). Cytochrome P-450 and non-mitochondrial oxygen metabolism in yeast. J. Appl. Chem. Biotech.,26: 50–51Google Scholar

Copyright information

© United Kingdom Environmental Mutagen Society 1985

Authors and Affiliations

  • I. G. de Mitchell
    • 1
  • P. J. Gilbert
    • 1
  1. 1.Beecham Pharmaceuticals Research DivisionStockUK

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