Abstract
The testing of four UKEMS trial compounds in the presence of Aroclor 1254-induced rat liver S9 using the standard Ames protocol showed that BZD and DAT induced mutation in S. typhimurium TA1538. A modification of the standard testing procedure known as the ‘York method’ demonstrated enhanced mutagenicity with BZD but not with DAT; Sample B of CDA was rendered a mutagen using this procedure. Optimum mutagenicity was seen with a final S9 concentration of 10%. Chicken liver S9 was less effective than rat in activating the two positive mutagens.
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Notes
Ames, B.N., McCann, J. and Yamasaki, E. (1975). Methods for detecting carcinogens and mutagens with the Salmonella/ mammalian-microsome mutagenicity test. Mutation Res. 31: 347–364
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© 1985 United Kingdom Environmental Mutagen Society
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Riley, S.E., Garner, R.C. (1985). The Testing of four UKEMS Trial Compounds for Bacterial Mutation in the Salmonella/Microsome Assay Using (1) Aroclor-induced Rat Liver, (2) Chicken Liver S9 and (3) The York Modification of the Standard Ames Protocol. In: Parry, J.M., Arlett, C.F. (eds) Comparative Genetic Toxicology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-07901-8_11
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DOI: https://doi.org/10.1007/978-1-349-07901-8_11
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-1-349-07903-2
Online ISBN: 978-1-349-07901-8
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