Abstract
Recent work has shown that the various extrafusal muscle fibre types distinguishable by means of their histochemical myosin-based properties all contain structurally slightly different forms (isoforms) of myosin. In nearly all cases a different histochemical profile is associated with the presence of a single distinct myosin isoform (Gauthier & Lowey, 1977; Pierobon-Bormioli et al., 1981; Rowlerson et al., 1981), but for IIC fibres there as biochemical and immunohistochemical evidence (Billeter et al., 1980 & 1981) that two myosin isoforms co-exist in the same fibres. Futhermore, in fibres which transform from one histochemical type to another there is a corresponding switch from one form of myosin to another (e.g. Rubinstein et al., 1978). In addition to histochemical methods for ATPase activity (e.g. Brooke & Kaiser, 1970) the various myosin isoforms can be detected immunohistochemically using type (isoform)-specific antibodies to myosin.
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References
Barany, M. (1967). ATPase activity of myosin correlated with speed of muscle shortening. J. Gen. Physiol., 50, 197–216.
Barker, D., Bessou, P., Jankowska, E., Pages, P. & Stacey, M.J. (1975). Distribution of static and dynamic axons to cat intrafusal muscle fibres. J. Anat. 119, 199–200.
Bessou, P. & Pages, B. (1972). Intracellular potentials from intrafusal muscle fibres evoked by stimulation of static and dynamic fusimotor axons in the cat. J. Phvsiol.. 227. 709–727.
Bessou, P. & Pages, B. (1975). Cinematographic analysis of contractile events produced in intrafusal muscle fibres by stimulation of static and dynamic fusimotor axons in the cat. J. Physiol., 252, 397–427.
Billeter, R., Weber, H., Lutz, H., Howald, H., Eppenberger, H.M. & Jenny, E. (1980). Myosin types in human skeletal muscle fibres. Histochemistry, 65, 249–259.
Billeter, R., Heizmann, C.W., Howald, H. & Jenny, E. (1981). Analysis of myosin light and heavy chain types in single human skeletal muscle fibres. Eur. J. Biochem. 116, 389–395.
Boyd, I.A. (1976). The response of fast and slow nuclear bag fibres and nuclear chain fibres in isolated cat muscle spindles to fusimotor stimulation, and the effect of intrafusal contraction on the sensory endings. Quart. J. Exp. Physiol., 61. 203–254.
Boyd, I.A., Gladden, M., McWilliam, P.N. & Ward, J (1977). Control of dynamic and static nuclear bag fibres by gamma and beta axons in isolated cat muscle spindles. J. Physiol., 265, 133–162.
Brooke, M.H. & Kaiser, K.K. (1970). Muscle fiber types: how many and what kind? Archs. Neurol., 23, 369–379.
Close, R. (1965). The relationship between intrinsic speed of shortening and duration of the active state in muscle. J. Physiol., 180, 542–549.
Gauthier, G.F. & Lowey, S. (1977). Polymorphism of myosin among skeletal muscle fiber types. J. Cell Biol. 74, 760–779.
Gladden, M. (1976). Structural features relative to the function of intrafusal muscle fibres in the cat. Prog. Brain Res. 44, 51–59.
Kronnie, G., Donselaar, Y., Soukup, T. & van Raamsdonk, W. (1981). Immunohistochemical differences in myosin composition among intrafusal muscle fibres. Histochemistry. 73. 65–74.
Ovalle, W.K. & Smith, R.S. (1972). Histochemical identification of three types of intrafusal muscle fibres in the cat and monkey based on the myosin ATPase reaction. Can. J. Physiol. Pharmacol., 50, 195–202.
Pierobon-Bormioli, S., Sartore, S., Vitadello, M. & Schiaffino, S. (1980). “Slow” myosins in vertebrate skeletal muscle. An immuno -fluorescence study. J. Cell Biol., 85, 672–681.
Pierobon-Bormioli, S., Sartore, S., Dalla Libera, L., Vitadello, M. & Schiaffino, S. (1981). “Fast” isomyosins and fiber types in mammalian skeletal muscle. J. Histochem. Cytochem., 29, 1179–1188.
Rowlerson, A., Pope, B., Murray, J., Whalen, R.B. & Weeds, A.G. (1981). A novel myosin present in cat jaw-closing muscles. J. Mus. Res. Cell Motil., 2, 415–438.
Rubinstein, N., Mabuchi, K., Pepe, F., Salmons, S., Gergely, J. & Sreter, F.A. (1978). Use of type-specific antimyosins to demonstrate the transformation of individual fibres in chronically stimulated rabbit fast muscles. J. Cell Biol., 79, 252–261.
Sartore, S., Gorza, L. & Schiaffino, S. (1982). Fetal myosin heavy chains in regenerating muscle. Nature 298, 294–296.
Whalen, R.G., Sell, S.M., Butler-Browne, G.S., Schwarz, K., Bouveret, P. & Pinset-Harstrom, I. (1981). Three myosin heavy chain isozymes appear sequentially in rat muscle development. Nature, 292, 805–809.
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© 1985 I. A. Boyd and M. H. Gladden
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Rowlerson, A., Gorza, L., Schiaffino, S. (1985). Immunohistochemical identification of spindle fibre types in mammalian muscle using type-specific antibodies to isoforms of myosin. In: Boyd, I.A., Gladden, M.H. (eds) The Muscle Spindle. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-07695-6_2
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DOI: https://doi.org/10.1007/978-1-349-07695-6_2
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