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Immunohistochemical identification of spindle fibre types in mammalian muscle using type-specific antibodies to isoforms of myosin

  • A. Rowlerson
  • L. Gorza
  • S. Schiaffino

Abstract

Recent work has shown that the various extrafusal muscle fibre types distinguishable by means of their histochemical myosin-based properties all contain structurally slightly different forms (isoforms) of myosin. In nearly all cases a different histochemical profile is associated with the presence of a single distinct myosin isoform (Gauthier & Lowey, 1977; Pierobon-Bormioli et al., 1981; Rowlerson et al., 1981), but for IIC fibres there as biochemical and immunohistochemical evidence (Billeter et al., 1980 & 1981) that two myosin isoforms co-exist in the same fibres. Futhermore, in fibres which transform from one histochemical type to another there is a corresponding switch from one form of myosin to another (e.g. Rubinstein et al., 1978). In addition to histochemical methods for ATPase activity (e.g. Brooke & Kaiser, 1970) the various myosin isoforms can be detected immunohistochemically using type (isoform)-specific antibodies to myosin.

Keywords

Myosin ATPase Activity Propagate Action Potential Chain Fibre Intrafusal Muscle Fibre Tonic Fibre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© I. A. Boyd and M. H. Gladden 1985

Authors and Affiliations

  • A. Rowlerson
  • L. Gorza
  • S. Schiaffino

There are no affiliations available

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