Abstract
The presence of dopamine (DA) receptors in the mammalian central nervous system (CNS) was first revealed in conventional in vitro binding assays using various radiolabelled DA agonists and neuroleptics as ligands (see Seeman, 1980 for review). However these biochemical techniques have a limited anatomical resolution In order to further our understanding of the functional organization of DA-containing systems (especially in relation to other neuronal pathways). DA receptors need to be localized more precisely within the different DA-rich brain regions. Recent methodological advances in receptor autoradiography (Young and Kuhar, 1979; Murrin 1981 Palacios et al., 1981 Rainbow et al., 1982; Unnerstall et al., 1982) have enabled the in vitro and in vivo binding of dopaminergic radioligands in the brain to be visualized and pharmacologically characterized with an anatomical resolution, both macroscopic and microscopic, unattainable by conventional radioligand binding techniques This approach combined with computerized microdensitometry can be used not only to map the distribution of receptors (or drug binding sites) in the CNS but also to quantify their regional densities.
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Scatton, B. et al. (1986). Autoradiographic Visualization and Quantification of Dopamine Receptors and Uptake Sites in the Rat and Human CNS. In: Woodruff, G.N., Poat, J.A., Roberts, P.J. (eds) Dopaminergic Systems and their Regulation. Satellite Symposia of the IUPHAR 9th International Congress of Pharmacology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-07431-0_8
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DOI: https://doi.org/10.1007/978-1-349-07431-0_8
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