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Radioimmunoassays in Analytical Methods in Human Toxicology

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Analytical Methods in Human Toxicology

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Abstract

As man has used more and more chemical substances in his daily life, the subject of human toxicology has necessarily become increasingly important. One of the highest causes of human toxicity from chemical substances is the use of drugs whether for therapeutic or other purposes. Since the introduction of more stringent regulations for the registration of drugs it has become necessary to study the pharmacokinetics and pharmacology of new (and old) drugs in great detail. There is also a greater awareness of the possible toxic effects of the many substances in every day use — both domestic and industrial. Concern about our environment is widespread.

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References

  • Aherne, G. W. (1983). The specificity of morphine radioimmunoassays. Internal Congress and Symposium Series of The Royal Society of Medicine. No. 58, 21–9.

    Google Scholar 

  • Aherne, G. W. (1984). Use and significance of immunoassay in the analysis of water. Analyt. Proc. 21, 177–9.

    CAS  Google Scholar 

  • Aherne, G. W., Lawson, G. J., Quinton, M., and Marks, V. (1984). Drug determination in therapeutic and forensic contexts. In Reid, E. and Wilson, I. D. (eds.), Methodological Surveys in Biochemistry and Analysis Vol. 14, Plenum, London, 249–50.

    Google Scholar 

  • Aherne, G. W., English, J., and Marks, V. (1984). The role of immunoassay in the analysis of micro-contaminants in water samples. Ecotox env. Safety 9, 79–83.

    Article  Google Scholar 

  • Aherne, G. W. (1982). The specificity of morphine radioimmunoassays. Intl. Cong. Symp. Ser. Roy. Soc. Med. 58, 21–9.

    Google Scholar 

  • Aherne, G. W., and Marks, V. (1982). A radioimmunoassay for VP-16–213 in plasma. Cancer Chemother. Pharmacol. 7, 117–21.

    CAS  PubMed  Google Scholar 

  • Aherne, G. W., James, S. L., and Marks, V. (1981). The radioiodination of bleomycin using iodogen. Clin. Chim. Acta 119, 341–3.

    Article  Google Scholar 

  • Aherne, G. W., Piall, E. M., and Marks, V. (1976). The radioimmunoassay of tricyclic antidepressants. Br. J. Clin. Pharmacol. 3, 561–5.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Albano, J., and Ekins, R. P. (1970). In Proceedings of the Symposium on in vitro Procedures with Radioisotopes in Clinical Medicine and Research. International Atomic Energy Agency, Vienna, 491–512.

    Google Scholar 

  • Bolton, A. E., and Hunter, W. H. (1973). The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem. J. 133, 529–39.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Burke, C. W., and Shakespear, R. A. (1975). Rapid purification of triiodothyronine and thyroxine protein conjugates for antibody production. J. Endocr. 65, 133–8.

    Article  CAS  PubMed  Google Scholar 

  • Butler, V. P., and Beiser, S. H. (1973). Antibodies to small molecules: biological and clinical applications. Adv. Immunol. 17, 255–310.

    Article  PubMed  Google Scholar 

  • Collins, W. P., Barnard, G. J., Kim, J. B., Weerasekera, D. A., Kohen, F., Eshhar, Z., and Lindner, H. R. (1983). In Hunter, W. M. and Corrie, J. G. T. (eds.), Immunoassays for Clinical Chemistry, Churchill Livingstone, Edinburgh, 373–97.

    Google Scholar 

  • Ekins, R. P. (1974). Basic principles and theory. Br. Med. Bull. 30, 3–11.

    CAS  PubMed  Google Scholar 

  • Ekins, R. (1981). In Voller, A., Bartlett, A., and Bidwell, D. (eds.), Immunoassays for the 80s, MTP Press, Lancaster, 5–17.

    Chapter  Google Scholar 

  • Ferrura, B., Milano, G., Ly, B., Guennec, J. Y., and Masseyeff, R. (1983). An enzyme immunoassay design using labelled antibodies for the determination of haptens: Application to methotrexate assay. J. Immunol. Methods 60, 257–69.

    Article  Google Scholar 

  • Findlay, J. W. A., Butz, R. F., and Jones, E. C. (1981). Relationships between immunogen structure and antisera specificity in the narcotic alkaloid series. Clin. Chem. 27, 1524–35.

    CAS  PubMed  Google Scholar 

  • Fraker, P. J., and Speck, J. C. (1978). Protein and cell membrane iodinations with a sparingly soluble chloroamide 1,3,4,6-tetrachloro-3α, 6α, diphenylglycouril. Biochem. Biophys. Res. Commun. 80, 849–57.

    Article  CAS  PubMed  Google Scholar 

  • Grabinski, P. Y., Kaiko, R. F., Walsh, T. D., Foley, K. M., and Houde, R. W. (1983). Morphine radioimmunoassay specificity before and after extraction of plasma and cerebrospinal fluid. J. Pharm. Sci. 72, 27–30.

    Article  CAS  PubMed  Google Scholar 

  • Greenwood, F C., Hunter, W. H., and Glover, J. S. (1963). The preparation of131I-labelled human growth hormone of high specific radioactivity. Biochem. J. 89, 114–23.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Harvey, M. H. (1983). The development and evaluation of a radioimmunoassay for the alkaloid solanidine in serum and the investigation of its levels in human serum. MSc Thesis, University of Surrey.

    Google Scholar 

  • Hunter, W. H., and Greenwood, F. C. (1962). Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194, 495–6.

    Article  CAS  PubMed  Google Scholar 

  • Hurn, B. A. L. (1974). Practical problems in raising antisera. Br. Med. Bull. 30, 26–8.

    Google Scholar 

  • Kamel, R. S., and Gardner, J. (1978). Preparation of125iodine-labelled methotrexate and its use in a magnetisable particle solid-phase radioimmunoassay. Clin. Chim. Acta 89, 363–70.

    Article  CAS  PubMed  Google Scholar 

  • Landon, J., and Moffat, A. C. (1976). The radioimmunoassay of drugs. Analyst 101, 225–43.

    Article  CAS  PubMed  Google Scholar 

  • Landsteiner, K. (1945). The Specificity of Serological Reactions, Harvard University Press, Boston. Reprinted by Dover, New York, 1962.

    Google Scholar 

  • Levitt, Y. (1977). Radioimmunoassay for paraquat. Lancet II, 358.

    Article  Google Scholar 

  • Lindstrom, L., Meurling, L., and Lovgren, T. (1982). The measurement of serum cortisol by a solid-phase chemiluminescence immunoassay. J. Steroid. Biochem. 16, 577–80.

    Article  CAS  PubMed  Google Scholar 

  • Lucek, R., and Dixon, R. (1977). Specific radioimmunoassay for amitriptyline and nortriptyline in plasma. Res. Commun. Chem. Pathol. Pharmacol. 18, 125–35.

    CAS  PubMed  Google Scholar 

  • Marks, V., Morris, B. A., and Teale, J. D. (1974). Pharmacology. Br. Med. Bull. 30, 80–6.

    CAS  PubMed  Google Scholar 

  • Mason, P. A., Law, B., and Ardney, R. E. (1981). Preparation of 2-iodomorphine for use in RIA. J. Lab. Comp. Radio-pharm. 18, 1497–507.

    Article  CAS  Google Scholar 

  • Mould, G. P., Stout, G., Aherne, G. W., and Marks, V. (1978). Radioimmunoassay of amitriptyline and nortriptyline in body fluids. Ann. Clin. Biochem. 15, 221–52.

    Article  CAS  PubMed  Google Scholar 

  • Mould, G. P., Clough, J., Morris, B. A., Stout, G., and Marks, V. (1981). A propranalol radioimmunoassay and its use in the study of its pharmacokinetics following low doses. Biopharm. Drug Disp. 2, 49–57.

    Article  CAS  Google Scholar 

  • Nordblom, G. D., Webb, R., Counsell, R. E., and England, B. G. (1981). A chemical approach to solving bridging phenomena in steroid radioimmunoassays. Steroids 38, 161–73.

    Article  CAS  PubMed  Google Scholar 

  • Okabayashi, T., Mihara, S., Repke, D. B., and Moffatt, J. G. (1977). A radioimmunoassay for 1-β-D-arabinofuranosyluracil with reference to cross-reactivity of 1-β-D-arabinofuranosylcytosine with an antibody. Cancer Research 37, 3132–5.

    CAS  PubMed  Google Scholar 

  • Oliver, G. C., Parker, B. H., Brasfield, D. L., and Parker, C. W. (1968). The measurement of digitoxin in human serum by radioimmunoassay. J. Clin. Invest. 47, 1035–42.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Piall, E. M., Aherne, G. W., and Marks, V. (1982). Evaluation of a commercially available radioimmunoassay kit for the measurement of doxorubin (adramycin) in plasma. Clin. Chem. 28, 119–21.

    CAS  PubMed  Google Scholar 

  • Ratcliffe, J. G., (1974). Separation techniques in saturation analysis. Br. Med. Bull. 30, 32–7.

    CAS  PubMed  Google Scholar 

  • Robinson, J. D., Morris, B. A., Piall, E. M., Aherne, G. W., and Marks, V. (1975). In Pasternak, C. A. (ed.), Radioimmunoassay in Clinical Biochemistry, Heyden, London, 101–13.

    Google Scholar 

  • Robinson, J. D., Risby, D., Riley, G., and Aherne, G. W. (1978). A radioimmunoassay for the determination of combined amitriptyline and nortriptyline concentrations in microlitre samples of plasma. J. Pharmacol. Exp. Ther. 205, 499–502.

    CAS  PubMed  Google Scholar 

  • Salacinsky, P., Hope, J., and McLean, C. (1979). A new simple method which allows theoretical incorporation of radioiodine into proteins and peptides without damage. J. Endoc. 81, 131.

    Article  Google Scholar 

  • Sizaret, P., Malaveille, C., Montesano, R., and Frayssinet, C. (1982). Detection of aflotoxins and related metabolites by radioimmunoassay. J. Natl. Cancer Inst. 69, 1375–81.

    CAS  PubMed  Google Scholar 

  • Slevin, M. L., Piall, E. M., Aherne, G. W., Johnston, A., Sweatman, M. C., and Lister, T. A. (1981). The pharmacokinetics of subcutaneous cytosine arabinoside in patients with acute myelogenous leukaemia. Br. J. Clin. Pharmac. 12, 507–10.

    Article  CAS  Google Scholar 

  • Smith, T. W., Butler, V. P., Haber, E., Fozzard, H., Marcus, F., Bremner, F., Schulman, I. and Phillips, A. (1982). Treatment of life-threatening digitalis intoxication with digoxin specific fab antibody fragments. New Engl. J. Med. 307, 1357–62.

    Article  CAS  PubMed  Google Scholar 

  • Smith, J., Pearson, S., and Marks, V. (1982). Plasma caffeine concentrations in outpatients. Lancet ii, 985–6.

    Article  Google Scholar 

  • Smith, R. N. (1983). Radioimmunoassay in forensic science. Analyt. Proc. 20, 417–19.

    CAS  Google Scholar 

  • Soini, E., and Hemmila, I. (1979). Fluoroimmunoassay: Present status and key problems. Clin. Chem. 25, 353–61.

    CAS  PubMed  Google Scholar 

  • Stoller, R. G., Hande, K. R., Jacobs, S. A., and Chabner, B. A. (1977). Use of plasma pharmacokinetics to predict and prevent methotrexate toxicity. New. Engl. J. Medicine 297, 630–4.

    Article  CAS  Google Scholar 

  • Teale, J. D., Clough, J. M., and Marks, V. (1977a). Radioimmunoassay of vinblastine and vincristine. Br. J. Clin. Pharmacol. 4, 160–72.

    Article  Google Scholar 

  • Teale, J. D., Clough, J. H., and Marks, V. (1977b). Radioimmunoassay of bleomycin in plasma and urine. Br. J. Cancer 33, 822–7.

    Article  Google Scholar 

  • Teale, J. D., Clough, J. M., King, L. J., Marks, V., Williams, P. L., and Moffat, A. C. (1977c). The incidence of cannabinoids in fatally injured drivers; an investigation by radioimmunoassay and high pressure liquid chromatography. J. Forens. Sci. Soc. 177–83.

    Google Scholar 

  • Thorell, J. I., and Johansson, B. G. (1971). Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim. Biophys. Acta 251, 363–9.

    Article  CAS  PubMed  Google Scholar 

  • Tigelaar, R. E., Rapport, R. L., Inman, J. K., and Kupferberg, H. J. (1973). A radioimmunoassay for diphenylhydantoin. Clin. Chim. Acta 43, 231–41.

    Article  CAS  PubMed  Google Scholar 

  • Vellejo, R. P., Bogus, E. R., and Mumma, R. O. (1982). Effects of hapten structure and bridging groups on antisera specificity in parathion immunoassay development. J. Agric. Food Chem. 30, 572–80.

    Article  Google Scholar 

  • Vining, R. F., Compton, P., and McGinley, R. (1981). Steroid radioimmunoassay — effect of shortened incubation time on specificity. Clin. Chem. 27, 910–13.

    CAS  PubMed  Google Scholar 

  • Voller, A., Bartlett, A., and Bidwell, D. E. (1978). Enzymeimmunoassays with special reference to ELISA techniques. J. Clin. Pathol. 31, 507–20.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Williams, P. L., Moffat, A. C., and King, L. J. (1978). Combined high-pressure liquid chromatography and radioimmunoassay method for the quantitation of delta-9-tetrahydrocannabinol and some of its metabolites in human plasma. J. Chromatogr. 155, 273–83.

    Article  CAS  PubMed  Google Scholar 

  • Yalow, R. S., and Berson, S. A. (1960). Immunoassay of endogenous plasma insulin in man. J. Clin. Invest. 39, 1157–75.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yalow, R. S., and Berson, S. A. (1970). In vitro Procedures with Radioisotopes in Clinical Medicine and Research. International Atomic Energy Agency, Vienna, 455–79.

    Google Scholar 

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Authors
  1. G. W. Aherne
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A. S. Curry

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Aherne, G.W. (1986). Radioimmunoassays in Analytical Methods in Human Toxicology. In: Curry, A.S. (eds) Analytical Methods in Human Toxicology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-07244-6_4

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