Significance of in vivo and in vitro metabolic studies of tumour-produced proteins

  • G. Mariani
  • P. W. Sullivan
  • E. A. McGuire
  • K. R. McIntire
  • R. H. Adamson
  • T. A. Waldmann


Recent years have witnessed growing interest and intense expectation in the areas of the immunodiagnosis of cancer and the evaluation (both for staging and for monitoring) of cancer patients. This is largely due to recent methodological advances in radioimmunoassay and other immunological techniques that are both highly sensitive and exquisitely specific. The number of tumour markers described in the literature is extremely high, whether this term is restricted to the purely oncodevelopmental, or oncofetal, proteins (those present in the biological fluids of the fetus and of patients with certain forms of cancer, but absent, or in exceedingly low concentrations, in the serum of normal adults) or extended to include the more general definition of tumour-produced, or tumour-related, proteins (such as, for instance, the Bence-Jones proteins of myeloma).


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Sullivan, P. W. and Salmon, S. E. (1972). ‘Kinetics of tumour growth and regression in IgG multiple myeloma’. J. Clin. Invest., 51, 1697.CrossRefGoogle Scholar
  2. 2.
    Waldmann, T. A., McIntire, K. R., Dalgard, D. W. and Adamson, R. H. (1976). ‘The metabolism of tumour related proteins’. In Plasma Protein Turnover (R. Bianchi, G. Mariani and A. S. McFarlane, Eds.), Macmillan, London, p. 155.Google Scholar
  3. 3.
    Viets, D. H., Howards, S. S. and Goldstein, G. (1977). ‘Serum alpha-fetoprotein in testicular tumors’. New Engl. J. Med., 296, 693.CrossRefGoogle Scholar
  4. 4.
    McIntire, K. R., Vogel, C. L., Primack, A., Waldmann, T. A. and Kyalwazi, S. K. (1976). ‘Effect of surgical and chemotherapeutic treatment on alpha-fetoprotein levels in patients with hepatocellular carcinoma’. Cancer, 37, 677.CrossRefGoogle Scholar
  5. 5.
    Waldmann, T. A. and McIntire, K. R. (1979). ‘The use of sensitive assays for alpha-fetoprotein in monitoring the treatment of malignancy’. In Immunodiagnosis of Cancer (R. B. Herberman and K. R. McIntire, Eds.), Marcel Dekker, New York, p. 130.Google Scholar
  6. 6.
    Vogelzang, N. J., Lange, P. H., Goldman, A., Vessela, R. H., Fraley, E. E. and Kennedy, B. J. (1982). ‘Acute changes of α-fetoprotein and human chorionic gonadotropin during induction chemotherapy of germ cell tumors’. Cancer Res., 42, 4855.Google Scholar
  7. 7.
    Rioche, M., Quelin, S. and Masseyeff, R. (1974). ‘Synthèse de protéines plasmatiques par les hépatocytes humains cancéreux et normaux en culture’. Pathol. Biol., 22, 867.Google Scholar
  8. 8.
    Szpirer, C. and Szpirer, J. (1975). ‘A mouse hepatoma cell line which secretes several serum proteins including albumin and α-fetoprotein’. Differentiation, 4, 85.CrossRefGoogle Scholar
  9. 9.
    Kelly, M. G., O’Gara, R. W., Adamson, R. H., Gadekar, K., Botkin, C. D., Reese, W. H., Jr. and Kerber, W. T. (1966). ‘Induction of hepatic cell carcinoma in monkeys with N-nitrosodiethylamine’. J. Natl Cancer Inst., 36, 323.Google Scholar
  10. 10.
    Sizaret, P. P., McIntire, K. R. and Princler, G. R. (1971). ‘Quantitation of human alpha-fetoprotein by electroimmunodiffusion’. Cancer Res., 31, 1899.Google Scholar
  11. 11.
    Waldmann, T. A. and McIntire, K. R. (1974). ‘The use of radioimmunoassay for alpha-fetoprotein in the diagnosis of malignancy’. Cancer, 34, 1510.CrossRefGoogle Scholar
  12. 12.
    Berman, M., Shahn, E. and Weiss, M. F. (1962). ‘The routine fitting of kinetic data to models: A mathematical formalism for digital computers’. Biophys. J., 2, 275.CrossRefGoogle Scholar
  13. 13.
    Berman, M. and Weiss, M. F. (1976). SAAM Manual (U.S. DHEW Publication No. NIH 76–730), U.S. Government Printing Office, Washington D.C.Google Scholar
  14. 14.
    Altman, P. L. (1961). Blood and Other Body Fluids. Biological Handbooks Series, Federation Am. Soc. Exp. Biol., Washington, D.C.Google Scholar
  15. 15.
    Anderson, W. B. and Pastan, I. (1975). ‘Altered adenylate cyclase activity: its role in growth regulation and transformation of fibroblasts’. In Advances in Nucleotide Research (G. I. Drummond, P. Greengard and G. A. Robinson, Eds.), Raven Press, New York, p. 681.Google Scholar
  16. 16.
    Pastan, I. H., Johnson, G. S. and Anderson, W. B. (1975). ‘Role of cyclic nucleotides in growth control’. Ann. Rev. Biochem., 44, 491.CrossRefGoogle Scholar
  17. 17.
    Johnson, G. S., Friedman, R. M. and Pastan, I. (1971). ‘Restoration of several morphological characteristics of normal fibroblasts in sarcoma cells treated with adenosine-3’, 5’-cyclic monophosphate and its derivatives. Proc. Natl Acad. Sci. U.S.A., 68, 425.CrossRefGoogle Scholar
  18. 18.
    Willingham, M. C. and Pastan, I. (1975). ‘Cyclic AMP modulates microvillous formation and agglutinability in transformed and normal mouse fibroblasts’. Proc. Natl Acad. Sci. U.S.A., 72, 1263.CrossRefGoogle Scholar
  19. 19.
    Dawe, C. J., Whang-Peng, J., Morgan, W. D., O’Gara, R. W. and Kelly, M. G. (1968). ‘Culture of a cell line (NCLP-6) derived from a hepatocarcinoma induced in Macaca mulatta by N-nitrosodiethylamine’. J. Natl Cancer Inst., 40, 1167.Google Scholar
  20. 20.
    Setaro, F. and Morley, C. G. D. (1976). ‘A modified fluorometric method for the determination of microgram quantities of DNA from cell or tissue cultures’. Analyt. Biochem., 71, 313.CrossRefGoogle Scholar
  21. 21.
    Gershengorn, M. C., Glinoer, D., Fox, S. H. and Robbins, J. (1976). ‘Thyroxine-binding globulin synthesis by hepatocarcinoma cells in continuous culture: effect of physiological concentrations of thyroxine’. Biochem. Biophys. Res. Commun., 71, 76.CrossRefGoogle Scholar
  22. 22.
    Bélanger, L., Hamel, D., Lachance, L., Dufour, D., Tremblay, M. and Gagnon, P. M. (1975). ‘Hormonal regulation of α1 -fetoprotein’. Nature, Lond., 256, 657.CrossRefGoogle Scholar
  23. 23.
    De Nechaud, B., Becker, J. E. and Potter, V. R. (1976). ‘Effect of glucocorticoids on fetoprotein production by an established cell line from Morris hepatoma 8994’. Biochem. Biophys. Res. Commun., 68, 8.CrossRefGoogle Scholar
  24. 24.
    Isaka, H., Umehara, S. and Tsukada, Y. (1975). ‘α-fetoprotein synthesis in cultured cells: Studies on Yoshida sarcoma and ascites hepatoma in vitro’. Ann N.Y. Acad. Sci., 259, 74.CrossRefGoogle Scholar
  25. 25.
    Tumyan, B. G., Svet-Moldavski, G. G. and Karmanova, N. V. (1975). ‘Factors suppressing α-fetoprotein production in newborn mice’. Nature, Lond., 255, 244.CrossRefGoogle Scholar
  26. 26.
    Kanai, K., Torii, M., Hayashi, S. and Watabe, H. (1979). ‘Effects of cyclic nucleotides on synthesis and release of alpha-fetoprotein by rat ascites hepatoma cells’. In Carcino-Embryonic Proteins (F. G. Lehmann, Ed.), Elsevier/North-Holland, Amsterdam, p. 219.Google Scholar
  27. 27.
    Bélanger, L., Chiu, J. F., Exton, J. H., Richardson, D., Laberge, C., Larochelle, J. and Bélanger, M. (1979). ‘Relation between ATP and AFP metabolism in liver injuries’. In Carcino-Embryonic Proteins (F. G. Lehmann, Ed.), Elsevier/North-Holland, Amsterdam, Vol. II, p. 223.Google Scholar
  28. 28.
    Watanabe, A., Miyazaki, M. and Nagashima, H. (1979). ‘Effect of pyridoxine-adenosine 5’-triphosphate and S-adenosylmethionine on increased alpha-fetoprotein synthesis by hepatic injury’. In Carcino-Embryonic Proteins (F. G. Lehmann, Ed.), Elsevier/North-Holland, Amsterdam, Vol. II, p. 231.Google Scholar
  29. 29.
    Kanai, K., Endo, Y., Oda, T. and Kaneko, Y. (1975). ‘Synthesis of α-fetoprotein by rat ascites hepatoma’. Ann. N.Y. Acad. Sci., 259, 29.CrossRefGoogle Scholar
  30. 30.
    van Vijk, R., Wicks, W. D. and Clay, K. (1972). ‘Effects of derivatives of cyclic 3’, 5’-adenosine monophosphate on the growth, morphology and gene expression of hepatoma cells in culture’. Cancer Res., 32, 1905.Google Scholar
  31. 31.
    Bélanger, L., Larochelle, J., Bélanger, M. and Privé, L. (1976). ‘Tyrosinosis: Hereditary persistence of alpha1-fetoprotein’. In Onco-Development Gene Expression (W. H. Fishmann and S. Sell, Eds.), Academic Press, New York, p. 155.Google Scholar

Copyright information

© The contributors 1984

Authors and Affiliations

  • G. Mariani
  • P. W. Sullivan
  • E. A. McGuire
  • K. R. McIntire
  • R. H. Adamson
  • T. A. Waldmann

There are no affiliations available

Personalised recommendations