Abstract
The rapid chromatographic resolution of proteins requires that the mobile phase must move with high flow rates, and therefore is usually forced through the microparticulate columns under pressure. Thus, the support material must be mechanically stable. Additionally, the material should be macroporous to allow sufficient molecular penetration of the gel and have adequate surface area for high loading capacity in adsorption-type chromatography.
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References
Beissner, R. S. and Rudolph, F. B. (1978). Arch. Biochem. Biophys., 189, 76
Bock, H. G., Skene, P., Fleischer, B., Cassidy, P. and Harshman, S. (1976). Science, N. Y., 191, 380
Chang, S. H., Gooding, K. M. and Regnier, F. E. (1976). J. Chromatogr., 125, 103
Clonis Y. D. (1982). J. Chromatogr., 236, 69
Clonis Y. D. (1987a). In HPLC of Macromolecules—A Practical Approach (ed. R. W. A. Oliver), IRL Press, Oxford
Clonis, Y. D. (1987b). J. Chromatogr. (in press)
Clonis, Y. D., Goldfinch, M. J. and Lowe, C. R. (1981). Biochem. J., 197, 203
Clonis, Y. D., Jones, K. and Lowe, C. R. (1986). J. Chromatogr., 363, 31
Clonis, Y. D. and Lowe, C. R. (1987a). Affinity chromatography. In Scientific Foundations of Clinical Biochemistry (ed. V. Marks and D. L. Williams), 2nd edn, Vol. 1, Heinemann Medical, London
Dean, P. D. G. and Watson, D. H. (1979). J. Chromatogr., 165, 301
Eketorp, R. (1982). In Affinity Chromatography and Related Techniques (ed. T. C. J. Gribnau, J. Visser and R. J. F. Nivard), p. 263, Elsevier, Amsterdam
Haller, W. (1973). J. Chromatogr., 85, 129
Holroyde, M. J., Chesher, J. M. E., Trayer, I. P. and Walker, D. G. (1976). Biochem. J., 153, 351
Hughes, P., Lowe, C. R. and Sherwood, R. F. (1982). Biochim. Biophys. Acta, 700, 90
Kramer, D. M. (1981). Proceedings 6th Enzyme Engineering Conference, Kashikojima, Japan
Larsson, P.-O. (1984). Methods Enzymol., 104, 212
Ledger, R. and Stellwagen, E. (1984). J. Chromatogr., 299, 175
Lowe, C. R. (1979). An introduction to affinity chromatography. In Laboratory Techniques in Biochemistry and Molecular Biology (ed. T. S. Work and E. Work), Vol. 7, pp. 267–522, Elsevier/North-Holland Biomedical Press, Amsterdam
Lowe, C. R. (1984). In Topics in Enzyme and Fermentation Biotechnology (ed. A. Wiseman), Vol. 9, pp. 78–161, Ellis Horwood, Chichester
Lowe, C. R., Larsson, P.-O., Ohlson, S., Small, D. A. P., Atkinson, T. and Mosbach, K. (1981). J. Chromatogr., 215, 303
Nilsson, K. and Mosbach, K. (1984). Methods Enzymol., 104, 56
Ohlson, S., Hansson, L., Larsson, P.-O. and Mosbach, K. (1978). FEBS Lett., 93, 5
Regnier, F. E. and Gooding, K. M. (1980). Analyt. Biochem., 103, 1
Regnier, F. E. and Noel, R. (1976). J. Chromatogr. Sci., 14, 316
Robinson, P. J., Wheatly, M. A., Janson, J. C., Dannill, P. and Lilly, M. D. (1974). Biotechnol. Bioengng, 16, 1103
Schmidt, D. E., Giese, R. W., Conron, D. and Karger, B. L. (1980). Analyt. Chem., 52, 177
Small, D.A.P., Atkinson, A. and Lowe, C. R. (1981). J. Chromatogr., 216, 175
Small, D.A.P., Atkinson, A. and Lowe, C. R. (1983). J. Chromatogr., 266, 151
Stout, R. W. and Destefano, J. J. (1985). J. Chromatogr., 326, 63
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Clonis, Y.D., Small, D.A.P. (1987). High-performance Dye-ligand Chromatography. In: Clonis, Y.D., Atkinson, T., Bruton, C.J., Lowe, C.R. (eds) Reactive Dyes in Protein and Enzyme Technology. Palgrave, London. https://doi.org/10.1007/978-1-349-06582-0_5
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DOI: https://doi.org/10.1007/978-1-349-06582-0_5
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