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Abstract

The rapid chromatographic resolution of proteins requires that the mobile phase must move with high flow rates, and therefore is usually forced through the microparticulate columns under pressure. Thus, the support material must be mechanically stable. Additionally, the material should be macroporous to allow sufficient molecular penetration of the gel and have adequate surface area for high loading capacity in adsorption-type chromatography.

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Clonis, Y.D., Small, D.A.P. (1987). High-performance Dye-ligand Chromatography. In: Clonis, Y.D., Atkinson, T., Bruton, C.J., Lowe, C.R. (eds) Reactive Dyes in Protein and Enzyme Technology. Palgrave, London. https://doi.org/10.1007/978-1-349-06582-0_5

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