Effects of xylosyladenine and other inhibitors of methylation on induction of murine erythroleukemia cells

  • Charles Garrett
  • Nicholas M Kredich


DNA methylation is of undetermined purpose in higher organisms, but a significant body of evidence suggests that it may be involved in cell differentiation (for a recent review see Razin and Riggs, 1980). Several examples have been reported where genes for tissue-specific proteins are less methylated in cells expressing these genes than in cells that do not (Waalwijk and Flavell, 1978; Mandel and Chambon, 1979; McGhee and Ginder, 1979; van der Ploeg and Flavell, 1980; Shen and Maniatis, 1980). Furthermore, some agents that interfere with DNA methylation can also induce differentiation in cultured cells (Christman, 1979; Constantinides et al., 1977, 1978; Christman et al., 1977 Taylor and Jones, 1979; Jones and Taylor, 1980).


Pyrimidine Nucleoside Hemoglobin Synthesis Methionine Adenosyltransferase Transmethylation Reaction Murine Erythroleukemia Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Borchardt, R.T. and Wu, Y.S. (1975). Potential Inhibitors of S-Adenosylmethionine-Dependent Methyltransferases. 3. Modifications of the Sugar Portion of S-Adenosylhomocysteine. J. Med. Chem. 18, 300–304.PubMedCrossRefGoogle Scholar
  2. Borchardt, R.T., Wu, Y.S., Huber, J.A. and Wycpalek, A.F. (1976). Potential Inhibitors of S-Adenosylmethionine-Dependent Methyltransferases. 6. Structural Modifications of S-Adenosylmethionine. J. Med. Chem. 19, 1104–1110.PubMedCrossRefGoogle Scholar
  3. Christman, J.K. (1979). DNA Methylation in Friend Erythroleukemia Cells. In Transmethylation, (eds. E. Usdin, R.T. Borchardt and C.R. Creveling), Elsevier/North Holland, New York.Google Scholar
  4. Christman, J.K., Price, P., Pedrinan, L. and Acs, G. (1977). Correlation Between Hypomethylation of DNA and Expression of Globin Genes in Friend Erythroleukemia Cells. Eur. J. Biochem. 81, 53–61.PubMedCrossRefGoogle Scholar
  5. Constantinides, P.G., Jones, P.A. and Gevers, W. (1977). Functional Striated Muscle Cells from Non-Myoblast Precursors Following 5-Azacytidine Treatment. Nature 267, 364–366.PubMedCrossRefGoogle Scholar
  6. Constantinides, P.G., Taylor, S.M. and Jones, P.A. (1978). Phenotypic Conversion of Cultured Mouse Embryo Cells by Aza Pyrimidine Nucleosides. Dev. Biol. 66, 57–71.PubMedCrossRefGoogle Scholar
  7. Friend, C., Scher, W., Holland, J.G. and Sato, T. (1971). Hemoglobin Synthesis in Murine Virus-Induced Leukemic Cells In Vitro: Stimulation of Erythroid Differentiation by Dimethyl Sulfoxide. Proc. Natl. Acad. Sci. USA 68, 378–382.PubMedPubMedCentralCrossRefGoogle Scholar
  8. Garrett, C. and Kredich, N.M. (1981). Induction of Hemoglobin Synthesis by Xylosyladenine in Murine Erythroleukemia Cells: Metabolism of Xylosyladenine and Effects on Transmethylation. J. Biol. Chem. in press.Google Scholar
  9. Jones, S.M. and Taylor, P.A. (1980). Cellular Differentiation, Cytidine Analogues and DNA Methylation. Cell 20, 85–93.PubMedCrossRefGoogle Scholar
  10. Kredich, N.M. (1980). Inhibition of Nucleic Acid Methylation by Cordycepin: In Vivo Synthesis of S-3’-Deoxyadenosylmethionine by WI-L2 Human Lymphoblasts. J. Biol. Chem. 255, 7380–7385.PubMedGoogle Scholar
  11. Kredich, N.M. and Guarino, A.J. (1960). An Improved Method of Isolation and Determination of Cordycepin. Biochim. Biophys. Acta 41, 363–365.PubMedCrossRefGoogle Scholar
  12. Kredich, N.M. and Hershfield, M.S. (1979). S-Adenosylhomocysteine Toxicity in Normal and Adenosine Kinase Deficient Lymphoblasts of Human Origin. Proc. Natl. Acad. Sci. USA 76, 2450–2454.PubMedPubMedCentralCrossRefGoogle Scholar
  13. Kredich, N.M. and Martin, D.W. Jr. (1977). Role of S-Adenosylhomocysteine in Adenosine Mediated Toxicity in Cultured Mouse T-Lymphoma Cells. Cell 12, 931–938.PubMedCrossRefGoogle Scholar
  14. Mandel, J.L. and Chambon, P. (1979). DNA Methylation: Organ Specific Variations in the Methylation Pattern Within and Around Ovalbumin and Other Chicken Genes. Nucl. Acids Res. 7, 2081–2103.Google Scholar
  15. McGhee, J.D. and Ginder, G.D. (1979). Specific DNA Methylation Sites in the Vicinity of the Chicken β-Globin Genes. Nature 280, 419–420.CrossRefGoogle Scholar
  16. Ohta, Y., Tanaka, M., Terada, M., Miller, O.J., Bank, A., Marks, P.A. and Rifkind, R.A. (1976). Erythroid Cell Differentiation: Murine Erythroleukemia Cell Variant with Unique Pattern of Induction by Polar Compounds. Proc. Natl. Acad. Sci. USA 73, 1232–1236.PubMedPubMedCentralCrossRefGoogle Scholar
  17. Razin, A. and Riggs, A.D. (1980). DNA Methylation and Gene Function. Science 210, 604–610.PubMedCrossRefGoogle Scholar
  18. Reuben, R.C., Rifkind, R.A. and Marks, P.A. (1980). Chemically Induced Murine Erythroleukemic Differentiation. Biochim. Biophys. Acta 605, 325–346.PubMedGoogle Scholar
  19. Shen, C.-K.J. and Maniatis, T. (1980). Tissue-Specific DNA Methylation in a Cluster of Rabbit β-Like Globin Genes. Proc. Natl. Acad. Sci. USA 77, 6634–6638.PubMedPubMedCentralCrossRefGoogle Scholar
  20. Singer, J., Roberts-Ems, J., Luthardt, F.W. and Riggs, A.D. (1979). Methylation of DNA in Mouse Early Embryos, Teratocarcinoma Cells and Adult Tissues of Mouse and Rabbit. Nuc. Acids Res. 7, 2369–2385.Google Scholar
  21. Taylor, S.M. and Jones, P.A. (1979). Multiple New Phenotypes Induced in 10T½ and 3T3 Cells Treated with 5-Azacytidine. Cell 17, 771–779.PubMedCrossRefGoogle Scholar
  22. van der Ploeg, L.H.T. and Flavell, R.A. (1980). DNA Methylation in the Human γδβ-Globin Locus in Erythroid and Nonerythroid Tissues. Cell 19, 947–958.PubMedCrossRefGoogle Scholar
  23. Waalwijk, C. and Flavell, R.A. (1978). DNA Methylation at a CCGG Sequence in the Large Intron of the Rabbit β-Globin Gene: Tissue-Specific Variations. Nucl. Acids Res. 5, 4631–4641.Google Scholar

Copyright information

© The contributors 1982

Authors and Affiliations

  • Charles Garrett
  • Nicholas M Kredich

There are no affiliations available

Personalised recommendations