The effects of methylation inhibitors on depolarization-dependent exocytosis, phospholipid methylation, and protein carboxymethylation in clonal pheochromocytoma cells

  • Carolyn S Rabe
  • Richard McGeeJr.

Abstract

Since transmethylations have been proposed as important components of a variety of transmembrane signaling processes (Borchardt, 1980) we have begun examining the role of transmethylations in depolarization-dependent exocytosis. We have chosen the clonal rat pheochromocytoma cell line PC12 as our model system. These cells store norepinephrine (NE) and acetylcholine (ACh) in secretory granules and release the transmitters in response to both K+ depolarization and nicotinic receptor stimulation in a fashion similar to sympathetic neurons (Greene and Rein, 1977; Rabe et al., 1980).

Keywords

Norepinephrine CHCl3 Methionine Choline Catecholamine 

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References

  1. Borchardt, R. T. (1980). S-Adenosyl-L-methionine-Dependent Macromolecule Methyltransferases: Potential Targets for the Design of Chemotherapeutic Agents. J. Med. Chem. 23,347–357.PubMedCrossRefGoogle Scholar
  2. Crews, F. T., Hirata, F., and Axelrod, J. (1980). Identification and Properties of Methyltransferases that Synthesize Phosphatidylcholine in Rat Brain Synaptosomes. J. Neurochem. 34,1491–1498.PubMedCrossRefGoogle Scholar
  3. Gagnon, C. (1979). Presence of a Protein Methylesterase in Mammalian Tissues. Biochem. Biophys. Res. Commun. 88,847–853.PubMedCrossRefGoogle Scholar
  4. Greene, L. A. and Rein, G. (1977). Release, storage and uptake of catecholamines by a clonal cell line of nerve growth factor (NGF) responsive pheochromocytoma cells. Brain Res. 129,247–263.Google Scholar
  5. Rabe, C. S., Williams, T. P. and McGee, R. Jr. (1980). Enhancement of Depolarization-Dependent Release of Norepinephrine by an Inhibitor of S-Adenosylmethionine-Dependent Transmethylations. Life Sci. 27,1753–1759.Google Scholar
  6. Zimmerman, T. P., Schmitges, C. J., Wolberg, G., Deeprose, R. D., Duncan, G. S., Cuatrecasas, P. and Elion, G. B. (1980). Modulation of cyclic AMP metabolism by S-adenosylhomocysteine and S-3-deazaadenosylhomocysteine in mouse lymphocytes. Proc. Natl. Acad. Sci. USA 77,5639–5643.PubMedPubMedCentralCrossRefGoogle Scholar

Copyright information

© The contributors 1982

Authors and Affiliations

  • Carolyn S Rabe
  • Richard McGeeJr.

There are no affiliations available

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