The complex nature of methyl-accepting chemotaxis proteins of enteric bacteria

  • Gerald L Hazelbauer
  • Peter Engström
  • Shigeaki Harayama


Methyl-accepting chemotaxis proteins (MCPs) are central to the mechanisms of chemotaxis in the enteric bacteria, Escherichia coli and Salmonella typhimurium. These proteins were the first physiological substrates to be identified for a protein carboxyl methyl transferase (Kort et al., 1975) and the functional significance of protein carboxyl methylation is best understood in the case of MCPs (Springer et al., 1979). Recent studies have revealed a striking complexityofstructure and function for the bacterial MCPs.


Alkali Treatment Enteric Bacterium Bacterial Chemotaxis Hybrid Plasmid Flagellar Motor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Boyd, A. and Simon, M. I. (1980). Multiple electrophoretic forms of methyl-accepting chemotaxis proteins generated by stimuluselicited methylation in Escherichia coli. J. Bacteriol. 143, 809–815.Google Scholar
  2. Boyd, A., Krikos, A. and Simon, M. (1981). Sensory transducers of E. coli are encoded by homologous genes. Cell, in press.Google Scholar
  3. Chelsky, D. and Dahlquist, R. W. (1980). Structural studies of methyl-acceping chemotaxis proteins of Escherichia coli: evidence for multiple methylation sites. Proc. Nat. Acad. Sci.:USA 77, 2434–2438.Google Scholar
  4. DeFranco, A. L. and Koshland, D. E., Jr. (1980). Multiple methylation in the processing of sensory signals during bacterial chemotaxis. Proc. Nat. Acad. Sci. USA 77, 2429–2433.Google Scholar
  5. De Jong, W. W., Zweers, A. and Cohen, L. H. (1978). Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complex. Biochem. Biophys. Res. Commun. 82, 523–539.Google Scholar
  6. Engström, P. and Hazelbauer, G. L. (1980). Multiple methylation of methyl-accepting chemotaxis proteins during adaptation of Escherichia coli to chemical stimuli. Cell 20, 165–171.Google Scholar
  7. Gagnon, C. (1979). Presence of a protein methylesterase in mammalian tissues. Biochem. Biophys. Res. Commun. 88, 847–853.Google Scholar
  8. Hazelbauer, G. L. and Engstrom, P. (1981). Multiple forms of methyl-accepting chemotaxis proteins distinguished by a factor in addition to multiple methylation. J. Bacteriol. 145, 35–42.Google Scholar
  9. Hazelbauer, G. L., Engstrom, P. and Harayama, S. (1981) Methylaccepting chemotaxis protein III and transducer gene trg. J. Bacteriol. 145, 43–49.Google Scholar
  10. Hazelbauer, G. L. and Harayama, S. (1979) Mutants in transmission of chemotactic signals from two independent receptors of E. coli. Cell 16, 617–625.Google Scholar
  11. Hedblom, M. L. and Adler, J. (1980) Genetic and biochemical properties of Escherichia coli mutants with defects in serine chemotaxis. J. Bacteriol. 144, 1048–1060.Google Scholar
  12. Koiwai, O., Minoshima, S. and Hayashi, H. (1980). Studies on bacterial chemotaxis. V. Possible involvement of four species of the methyl-accepting chemotaxis protein in chemotaxis of Escherichia coli. J. Biochem. 87, 1365–1370.Google Scholar
  13. Kort, E. N., Goy, M. F., Larsen, S. H. and Adler, J. (1975). Methylation of a membrane protein involved in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 72, 3939–3943.Google Scholar
  14. Noel, D., Nikaido, K. and Ames, G. F.-L. (1979). A single amino acid substitution in a histidine transport protein drastically alters its mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochemistry 18, 4159–4165.Google Scholar
  15. Rollins, C. and Dahlquist, F. W. (1981). The methyl-accepting chemotaxis proteins of E. coli: a repellent-stimulated covalent modification, distinct from methylation. Cell 25, 333–340.Google Scholar
  16. Sherris, D. and Parkinson, J. S. (1981). Posttranslational processing of methyl-accepting chemotaxis proteins in Escherichia coli. Proc. Nat. Acad. Sci. USA 78, in press.Google Scholar
  17. Springer, M. S., Goy, M. F. and Adler, J. (1979). Protein methylation in behavioral control mechanisms and in signal transduction. Nature 280, 279–284.Google Scholar
  18. Wang, E. A. and Koshland, D. E., Jr. (1980). Receptor structure in the bacterial sensing system. Proc. Nat. Acad. Sci. USA 77, 7157–7161.Google Scholar

Copyright information

© The contributors 1982

Authors and Affiliations

  • Gerald L Hazelbauer
    • 1
  • Peter Engström
    • 1
  • Shigeaki Harayama
    • 2
  1. 1.Biochemistry/Biophysics ProgramWashington State UniversityPullmanUnited States
  2. 2.Laboratory of Genetics, Department of BiologyFaculty of Science, University of TokyoHongoJapan

Personalised recommendations