Catecholamine synthesizing enzymes in rat pheochromocytoma PC-12 cells

  • Esther Sabban
  • Jim Fong
  • Keith Markey
  • Louis Shenkman
  • Menek Goldstein
  • Lloyd A Greene

Abstract

The purification of catecholamine synthesizing enzymes from rat adrenal glands or brain requires a large number of animals and elaborate procedures are yielding small amounts of purified enzyme preparations. Furthermore, rat adrenal tyrosine hydroxylase (TH) tends to aggregate and this hampers its extensive purification. The availability of transplantable rat pheochromocytoma tumors (Warren and Chute, 1972), and of cultured rat pheochromocytoma cells (Tischler and Greene, 1975), provides an excellent source for purification of rat catecholamine synthesizing enzyme. Indeed, we have recently purified TH from a clonal pheochromocytoma PC-12 cell line (Markey et al., 1979) and dopaminebeta-hydroxylae (DβH) from rat pheochromocytoma tumors (Fong et al., 1980). In this presentation, we will review the characteristics of rat pheochromocytoma TH and DβH and we will describe some of our recent studies with DβH in cultured PC-12 cells.

Keywords

Sucrose Dopamine Tyrosine Sedimentation Electrophoresis 

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References

  1. Drummond, G.S., Symchowicz, E., Goldstein, M. and Shenkman, L. (1978). J. Neural Transmission, 42, 139.CrossRefGoogle Scholar
  2. Fong, J.C., Shenkman, L. and Goldstein, M. (1980). Journal of Neurochemistry. 34. 346.CrossRefPubMedGoogle Scholar
  3. Markey, K.A., Kondo, S., Shenkman, L. and Goldstein, M. (1980). Molecular Pharmacology, 17, 79.PubMedGoogle Scholar
  4. Tischler, A.S. and Greene, L.A. (1975). Nature, 258, 341.CrossRefPubMedGoogle Scholar
  5. Warren, S. and Chute, R. (1972). Cancer, 29, 327.CrossRefPubMedGoogle Scholar
  6. This study is supported by NINDS grant #06801 and NIMH grant #02717.Google Scholar

Copyright information

© The Contributors 1981

Authors and Affiliations

  • Esther Sabban
    • 1
  • Jim Fong
    • 1
  • Keith Markey
    • 1
  • Louis Shenkman
    • 2
  • Menek Goldstein
    • 1
  • Lloyd A Greene
    • 3
  1. 1.Departments of PsychiatryNew York University Medical CenterNew YorkUnited Kingdom
  2. 2.Departments of MedicineNew York University Medical CenterNew YorkUnited Kingdom
  3. 3.Departments of PharmacologyNew York University Medical CenterNew YorkUnited Kingdom

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