Combined immunocytochemical and radioautographic study of dopaminergic terminals in the neostriatum
Immunocytochemical localization of tyrosine hydroxylase alone or in combination with radioautographic localization of 3H-dopamine is used to determine the ultrastructural morphology and synaptic specializations of dopaminergic terminals in the n. caudatus-putamen (neostriatum) of adult rat brain. In all regions of the neostriatum, the enzyme immunoreactivity is localized to unmyelinated axons and axon terminals having a diameter of 0.1–1.5 µm. The most prevalent type of terminal is 0.15-0.8µm in diameter and forms symmetric junctions with unlabeled dendrites and dendritic spines. In ventricular regions, the tyrosine hydroxylase labeled axon terminals exhibit selective radioautographic labeling for 3H-dopamine, thus establishing the specificity of uptake and synthesis mechanisms within the same axons.
KeywordsHydroxyl Dopamine Tyrosine Citrate Aldehyde
Unable to display preview. Download preview PDF.
- Reaudet, A., Pickel V., Joh, T.H., Reis, D.J. and Cuenod, M. (1981). Combined radioautographic localization of 3H-mono-amines and immunocytochemical localization of tyrosine hydroxylase in brain. J. Histochem. Cytochem., submitted.Google Scholar
- Pickel, V.M., Beckley, S.C., Joh, T.H., Reis, D.J. (1981). Ultrastructural localization of tyrosine hydroxylase in neostriatum. Brain Res., submitted.Google Scholar
- Pickel, V.M., Sumal, K.K., Beckley, S.C., Miller, R.J., and Reis, D.J. (19800. Immunocytochemical localization of enkephalin in the neostriatum of rat brain: a light and electron microscopic study. J. Comp. Neurol., 189, 721–740.Google Scholar
- Pickel, V.M., Joh, T.H. and Reis, D.J. (1977). Regional and ultrastructural localization of tyrosine hydroxylase by immunocytochemistry in dopaminergic neurons of the mesolimbic and nigroneostriatal system. In Advances in Biochemical Psychopharmacology, Vol. 16 (eds. E. Costa and G.L. Gessa), Raven Press, New York, 321–329.Google Scholar
- Sternberger, L.A. (1974). Immunocytochemistry, Prentice Hall, Englewood Cliffs, N.J..Google Scholar