Combined immunocytochemical and radioautographic study of dopaminergic terminals in the neostriatum

  • V M Pickel
  • A Beaudet
  • T H Joh
  • D J Reis
  • M Cuenod

Abstract

Immunocytochemical localization of tyrosine hydroxylase alone or in combination with radioautographic localization of 3H-dopamine is used to determine the ultrastructural morphology and synaptic specializations of dopaminergic terminals in the n. caudatus-putamen (neostriatum) of adult rat brain. In all regions of the neostriatum, the enzyme immunoreactivity is localized to unmyelinated axons and axon terminals having a diameter of 0.1–1.5 µm. The most prevalent type of terminal is 0.15-0.8µm in diameter and forms symmetric junctions with unlabeled dendrites and dendritic spines. In ventricular regions, the tyrosine hydroxylase labeled axon terminals exhibit selective radioautographic labeling for 3H-dopamine, thus establishing the specificity of uptake and synthesis mechanisms within the same axons.

Keywords

Hydroxyl Dopamine Tyrosine Citrate Aldehyde 

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Copyright information

© The Contributors 1981

Authors and Affiliations

  • V M Pickel
    • 1
  • A Beaudet
    • 2
  • T H Joh
    • 1
  • D J Reis
    • 1
  • M Cuenod
    • 3
  1. 1.Department of NeurologyCornell University Medical CollegeNew YorkUSA
  2. 2.Laboratory of NeuroanatomyMontreal Neurological InstituteMontrealCanada
  3. 3.Brain Research InstituteUniversity of ZurichZurichSwitzerland

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