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Coexistence of adenosine 5′-triphosphate and acetylcholine in the electromotor synapse

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Co-Transmission

Abstract

The neurotransmitter acetylcholine (ACh) is released from the presynaptic nerve terminal in the form of quantal packets. After diffusing through the synaptic cleft, it acts at the postsynaptic receptor. In the presynaptic nerve terminal ACE is stored inside synaptic vesicles which are thought to be the subcellular manifestation of quantal packaging. There is a “choline cycle” at the nerve terminal: ACh released is hydrolysed to form choline and acetate. An active transport system of high affinity supplies the nerve terminal with extracellular choline for renewed synthesis and release of ACh. Particularly for neuromuscular transmission these mechanisms would appear to be satisfactory for maintaining physiological levels of activation of the effector system.

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References

  • Barberis, C., Minn, A. and Gayet, J. (1981). Adenosine transport into guinea-pig synaptosomes. J. Neurochem., 36, 347–54

    Article  Google Scholar 

  • Bender, A. S., Wu, P. H. and Phillis, J. W. (1981). The rapid uptake and release of 3 H-adenosine by rat cerebral cortical slices. J. Neurochem., 36, 651–60

    Article  Google Scholar 

  • Burnstock, G. (1975). Purinergic transmission. In Handbook of Psychopharmacology, 5, (ed. Iversen, L. L., Iversen, S. D. and Snyder, S. H.), Plenum Press, New York, pp. 131–94

    Google Scholar 

  • Dowdall, M. J. (1978). Adenine nucleotides in cholinergic transmission: presynaptic aspects. J. Physiol. Paris, 74, 497–501

    Google Scholar 

  • Dowdall, M. J., Boyne, A. F. and Whittaker, V. P. (1974). Adenosine triphospahte, a constituent of cholinergic synaptic vesicles. Biochem. J., 140, 1–12

    Article  Google Scholar 

  • Forrester, T. and Williams, C. A. (1977). Release of adenosine triphosphate from adult heart cells in response to hypoxia. J. Physiol. Lond., 268, 371–90

    Article  Google Scholar 

  • Fredholm, B. B. and Vernet, L. (1979). Release of 3 H-nucleosides from 3 H-labelled hypothalamic synaptosomes. Acta Physiol. Scand. 106, 97–107

    Article  Google Scholar 

  • Fredholm, B. B. and Hedquist, P. (1980). Modulation of neurotransmission by purine nucleotides and nucleosides. Biochem. Pharmac., 29, 1635–43

    Article  Google Scholar 

  • Giompres, P. E., Zimmermann, H. and Whittaker, V. P. (1981a) Purification of small dense vesicles from stimulated Torpedo electric tissue by glass bead column chromatography. Neuroscience, 6, 765–74

    Article  Google Scholar 

  • Giompres, P. E., Zimmermann, H. and Whittaker, V. P. (1981b). Changes in the biochemical and biophysical parameters of cholinergic synaptic vesicles on transmitter release and during a subsequent period of rest. Neuroscience, 6, 775–85

    Article  Google Scholar 

  • Ingvar, D. H. (1976). Functional landscapes of the dominant hemisphere. Brain Res., 107, 181–97

    Article  Google Scholar 

  • Israel, M. and Neunier, F. M. (1978). The release of ATP triggered by transmitter action and its possible physiological significance: retrograde transmission. J. Physiol. Paris, 74, 485–490

    Google Scholar 

  • Kato, A. C. Katz, H. S. and Collier, B. (1974). Absence of adenine nucleotide release from autonomic ganglion. Nature, London, 249, 576–77

    Article  Google Scholar 

  • Kilbinger, H. and Wessler, I. (1980). Inhibition by acetylcholine of the stimulation-evoked release of 3H-acetylcholine from the guinea-pig myenteric plexus. Neuroscience, 5, 1331–40

    Article  Google Scholar 

  • Kloog, Y., Michaelson, D. M. and Sokolovsky, M. (1980). Characterization of the presynaptic muscarinic receptor in synaptosomes of Torpedo electric organ by means of equilibrium binding studies. Brain Res. 194, 97–115

    Article  Google Scholar 

  • Lugmani, Y. A. (1981). Nucleotide uptake by isolated cholinergic synaptic vesicles: evidence for a carrier of adenosine 5′-triphosphate. Neuroscience, 6, 1011–22

    Article  Google Scholar 

  • Meunier, F. M. and Morel, N. (1978). Adenosine uptake by cholinergic synaptosomes from Torpedo electric organ. J. Neurochem., 31, 845–851

    Article  Google Scholar 

  • Michaelson, D. M. (1978). Is presynaptic acetylcholine release accompanied by secretion of the synaptic vesicles contents? FEBS Lett. 89, 51–53

    Article  Google Scholar 

  • Morel, N. and Meunier, F. M. (1981). Simultaneous release of acetylcholine and ATP from stimulated cholinergic synaptosomes. J. Neurochem. 36, 1766–73

    Article  Google Scholar 

  • Murray, A. W. (1971). The biological significance of purine salvage. A. Rev. Biochem. 40, 811–26

    Article  Google Scholar 

  • Nagy, A., Baker, R. R., Morris, S. J. and Whittaker, V. P. (1976). The preparation and characterization of synaptic vesicles of high purity. Brain Res., 109, 285–309

    Article  Google Scholar 

  • Nagy, A., Várady, G., Joo, F., Rakonczay, Z. and Pill, A. (1977). Separation of acetylcholine and catecholamine containing synaptic vesicles from brain cortex. J. Neurochem., 29, 449–59

    Article  Google Scholar 

  • Newman, M. E., Patel, J. and McIlwain, H. (1981). The binding of 3 H-adenosine to synaptosomal and other preparations from mammalian brain. Biochem. J., 194, 611–20

    Article  Google Scholar 

  • Ohsawa, K., Dowe, G. H. C., Morris, S. J. and Whittaker, V. P. (1979). The lipid and protein content of cholinergic synaptic vesicles from the electric organ of Torpedo marmorata purified to constant composition: implications for vesicle structure. Brain Res., 161, 447–57

    Article  Google Scholar 

  • Potter, P. and White, T. D. (1980). Release of adenosine 5′-triphosphate from synaptosomes from different regions of rat brain. Neuroscience, 5, 1331–56

    Article  Google Scholar 

  • Pull, I. and McIlwain, H. (1977). Adenine nucleotides and their metabolites liberated from and applied to isolated tissues of the mammalian, brain. Neurochem. Res., 2, 203–16

    Article  Google Scholar 

  • Richardson, P. J. and Whittaker, V. P. (1981). The Na+ and K+ content of isolated Torpedo synaptosomes and its effect on choline uptake. J. Neurochem. 36, 1536–42

    Article  Google Scholar 

  • Sawynok, J. and Jahmadas, K. H. (1976). Inhibition of acetylcholine release from cholinergic nerves by adenosine, adenine nucleotides and morphine: antagonism by theophylline. J. Pharmac. exp. Therap., 197, 379–90

    Google Scholar 

  • Sheridan, M. N., Whittaker, V. P. and Israel, M. (1966). The subcellular fractionation of the electric organ of Torpedo. Z. Zellforsch. 74, 291–307

    Article  Google Scholar 

  • Tashiro, T. and Stadler, H. (1978). Chemical compositions of cholinergic synaptic vesicles from Torpedo marmonata based on improved purification. Eur. J. Biochem., 90, 479–487

    Article  Google Scholar 

  • Wagner, J. A., Carlson, S. S. and Kelly, R. B. (1978). Chemical and physical characterization of cholinergic synaptic vesicles. Biochemistry, 17, 1199–1206

    Article  Google Scholar 

  • White, T., Potter, P. and Wonnacot, S. (1980). Depolarization induced release of ATP from cortical synaptosomes is not associated with acetylcholine release. J. Neurochem., 34, 1109–12

    Article  Google Scholar 

  • Wolfe, M. M. and Berne, R. M. (1956). Coronary vasodilator properties of purine and pyrimidine derivatives. Circulat. Res., 4, 343–48

    Article  Google Scholar 

  • Zimmermann, H. (1978). Turnover of adenine nucleotides in cholinergic synaptic vesicles of the Torpedo electric organ. Neuroscience, 3., 827–36

    Article  Google Scholar 

  • Zimmermann, H. (1979a). Vesicle recycling and transmitter release. Neuroscience, 4, 1773–804

    Article  Google Scholar 

  • Zimmermann, H. (1979b). Vesicular heterogeneity and turnover of acetylcholine and ATP in cholinergic synaptic vesicles. Progr. Brain Res., 49, 141–51

    Article  Google Scholar 

  • Zimmermann, H. (1982). Isolation of cholinergic nerve vesicles. In Neurotransmitter vesicles, (ed. R. Klein, H. Lagercrantz and H. Zimmermann)., Academic Press, London, pp. 241–269

    Google Scholar 

  • Zimmermann, H. and Whittaker, V. P. (1974). Effect of electrical stimulation on the yield and composition of synaptic vesicles from the cholinergic synapses of the electric organ of Torpedo: a combined biochemical, electrophysiological and morphological study. J. Neurochem., 22, 435–50

    Article  Google Scholar 

  • Zimmermann, H. and Denston, C. R. (1976). Adenosine triphosphate in cholinergic vesicles isolated from the electric organ of electrophorus electricus. Brain Res., 111, 365–76

    Article  Google Scholar 

  • Zimmermann, H. and Denston, C. R. (1977a). Recycling of synaptic vesicles in the cholinergic synapses of the Torpedo electric organ during induced transmitter release. Neuroscience, 2, 695–714

    Article  Google Scholar 

  • Zimmermann, H. and Denston, C. R. (1977b). Separation of synaptic vesicles of different functional states from the cholinergic synapses of the Torpedo electric organ. Neuroscience, 2, 715–30

    Article  Google Scholar 

  • Zimmermann, H. and Bokor, J. T. (1979). ATP recycles independently of ACh in cholinergic synaptic vesicles. Neurosci. Lett., 13, 319–24

    Article  Google Scholar 

  • Zimmermann, H., Dowdall, M. J. and Lane, D. A. (1979). Purine salvage at the cholinergic nerve endings of the Torpedo electric organ: the central role of adenosine. Neuroscience, 4, 979–993

    Article  Google Scholar 

  • Zimmermann, H., Stadler, H. and Whittaker, V. P. (1981). Structure and function of cholinergic synaptic vesicles. In Chemical Neurotransmission: 75 Years (ed. L. Stjärne, P. Hedquist, H. Lagercrantz and Å. Wennmalm), Academic Press, London, pp. 91–104

    Google Scholar 

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Author information

Authors and Affiliations

  1. Fachbereich Biologie der Universität Oldenburg, Postfach 2503, D-2900, Oldenburg, Germany

    H. Zimmermann

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  1. H. Zimmermann
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Editor information

Editors and Affiliations

  1. University Department of Pharmacology, Oxford, UK

    A. C. Cuello

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© 1982 The Contributors

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Zimmermann, H. (1982). Coexistence of adenosine 5′-triphosphate and acetylcholine in the electromotor synapse. In: Cuello, A.C. (eds) Co-Transmission. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-06239-3_12

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