Characterization of Human Brain Phenol Sulfotransferase

  • Jerome A. Roth
  • Jennifer Rivett
  • Kevin Renskers


The biogenic amine neurotransmitters are inactivated in the central nervous system by several enzymatic reactions including deamination by monoamine oxidase (MAO), 0-methylation by catechol-0-methyltransferase (COMT) and 0-sulfation by phenol sulfotransferase (PST). The properties and location of both MAO and COMT have been extensively studied in human brain whereas the enzyme or enzymes involved in sulfate conjugation have received relatively little attention. Recent evidence (Anderson and Weinshilboum, 1980; Tyce et al., 1980; Renskers et al., 1980) has suggested that sulfate conjugation may contribute significantly to the overall inactivation of the putative amine neurotransmitters, especially dopamine. However, quantitative information as to the magnitude of this enzymatic process in human brain has not been assessed.


Human Brain Kinetic Constant Total Enzymatic Activity Crude Homogenate COMT Activity 
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  1. Anderson, R.J. and Weinshilboum, R.M. (1980). Phenolsulfotransferase in Human Tissue: Radiochemical Enzymatic Assay and Biochemical Properties. Clin. Chim. Acta, 103, 79–90.CrossRefPubMedGoogle Scholar
  2. Bronaugh, R.L., Hattox, S.E., Hoehn, M.M., Murphy, R.C. and Rutledge, C.O. (1975). The Separation and Identification of Dopamine 3–0-Sulfate and Dopamine 4–0-Sulfate in Urine of Parkinsonian Patients. J. Pharmac.ol. Exp. Ther., 195, 441–452.Google Scholar
  3. Foldes, A. and Meek, J.L. (1973). Rat Brain Phenolsulfotransferase — Partial Purification and Some Properties. Biochim. Biophys. Acta, 327, 365–374.CrossRefPubMedGoogle Scholar
  4. Goodall, M. and Alton, H. (1972). Metabolism of 3,4-Dihydroxyphenylalanine (L-DOPA) in Human Subjects. Biochem. Pharmacol., 21, 2401–2408.CrossRefPubMedGoogle Scholar
  5. Haggendal, J. (1963). The Presence of Conjugated Adrenaline and Noradrenaline in Human Blood Plasma. Acta Physiol. Scand., 59, 255–260.Google Scholar
  6. Hart, R.F., Renskers, K.J., Nelson, E.B. and Roth, J.A. (1979). Localization and Characterization of Phenol Sulfotransferase in Human Platelets. Life Sci., 24, 125–130.CrossRefPubMedGoogle Scholar
  7. Jenner, W.N. and Rose, F.A. (1974). Dopamine-3–0-Sulfate, an End Product of L-DOPA Metabolism in Parkinson Patients. Nature, 252, 237–238.CrossRefPubMedGoogle Scholar
  8. Johnson, C.A., Baker, C.A. and Smith, R.T. (1980). Radioenzymatic Assay of Sulfate Conjugates of Catecholamines and DOPA in Plasma. Life Sci., 26, 1591–1598.CrossRefPubMedGoogle Scholar
  9. Kahone, Z., Essner, A.H., Kline, N.S, and Vestergaard, P. (1967). Estimation of Conjugated Epinephrine and Norepinephrine in Urine. J. Lab. Clin. Med., 69, 1042–1050.Google Scholar
  10. Pennings, E.J.M., Vrielink, R. and VanKempen, G.M.J. (1978). Kinetics and Mechanism of the Rat Brain Phenol Sulfotransferase Reaction. Biochem. J., 173, 299–307.PubMedCentralCrossRefPubMedGoogle Scholar
  11. Renskers, K.J., Feor, K.D. and Roth, J.A. (1980). Sulfation of Dopamine and Other Biogenic Amines by Human Brain Phenol Sulfotransferase. J. Neurochem., 34, 1362–1368.CrossRefPubMedGoogle Scholar
  12. Richter, D. (1940). The Inactivation of Adrenaline in Vivo in Man. J. Physiol., 98, 361–374.PubMedCentralCrossRefPubMedGoogle Scholar
  13. Richter, D. and MacIntosh, F.C. (1941). Adrenaline Ester. Am. J. Physiol., 135, 1–5.Google Scholar
  14. Roth, J.A. (1980). Presence of Membrane-Bound Catechol-O-Methyltransferase in Human Brain. Biochem. Pharmacol., 29, 3119–3122.CrossRefPubMedGoogle Scholar
  15. Roth, J.A. and Feor, K.D. (1978). Deamination of Dopamine and its 3–0-Methylated Derivative by Human Brain Monoamine Oxidase. Biochem. Pharmacol., 27, 1606–1608.CrossRefPubMedGoogle Scholar
  16. Rutledge, C.O. and Hoehn, M.M. (1973). Sulphate Conjugation and L-DOPA Treatment of Parkinsonian Patients. Nature, 244, 447–450,CrossRefPubMedGoogle Scholar
  17. Tyce, B.M., Sharpless, N.S., Kerr, F.W.L. and Muenter, M.D. (1980), Dopamine Conjugate in Cerebrospinal Fluid. J. Neurochem., 34, 210–212,CrossRefPubMedGoogle Scholar
  18. White, H.L. and Glassman, A.T. (1977). Multiple Binding Sites of Human Brain and Liver Monoamine Oxidase: Substrate Specificities, Selective Inhibitors, and Attempts to Separate Enzyme Forms. J. Neurochem., 29, 987–997.CrossRefPubMedGoogle Scholar

Copyright information

© The Contributors 1981

Authors and Affiliations

  • Jerome A. Roth
    • 1
  • Jennifer Rivett
    • 1
  • Kevin Renskers
    • 1
  1. 1.Department of Pharmacology and TherapeuticsSUNY at BuffaloBuffaloUSA

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