Regulation of 5-HT Receptor Binding as a Biochemical model for Pathological or Functional Changes in the CNS
Chemically mediated neurotransmission and the transmitter-receptor interaction depend on the amount of transmitter molecules released as quantal units into the synaptic cleft [Del Castillo and Katz, 1955]. Aside from transmitter concentration also the quality and quantity of receptor molecules will affect the transmission process. Though biochemical in vitro studies are restricted to the characterization of ligand-receptor binding which can supply only limited information about the possible physiological response, the measurement of binding kinetics has proved to be a suitable tool for describing central dysfunctions on the molecular level. After cerebral insult a significant decrease in 5-HT binding was observed in membranes isolated from the infarct region as compared with fractions obtained from intact regions or from control postmortem brain [Weiner et al., 1979]. The possibility that changes in ligand-receptor interaction reflect merely generalized necrosis or postmortem changes seems unlikely in the light of studies on 5-HT binding in Huntington’s chorea [Enna et al., 1976].
KeywordsTourette Syndrome Receptor Sensitivity Crude Membrane Crude Membrane Fraction Cerebral Insult
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