Interaction of fluorescent analogues of acetylcholine with nicotinic acetylcholine receptors and acetylcholine esterase

  • Heino Prinz
  • Rolf Jürss
  • Alfred Maelicke
Chapter
Part of the Biological Council book series

Abstract

Time-resolved electrophysiological studies of end-plate responses to acetylcholine and cholinergic agonists define the time range for molecular studies in which the events at the neuromuscular junction may be simulated in vitro. Since this time range is of the order of milliseconds, rapid kinetic techniques have to be employed. Suitable ligands for such studies require the following combination of properties: (1) they must have suitable spectroscopic properties, such as fluorescence with high quantum yield, and excitation and emission remote from the ultraviolet-absorption range of proteins; (2) their pharmacological properties must closely resemble those of the natural transmitter and, in particular, must be uncomplicated by side effects which may obscure interpretation of kinetic data.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fertuck, H. C. and Salpeter, M. M. (1976). J. Cell Biol., 69, 144–58CrossRefGoogle Scholar
  2. Heidmann, T. and Changeux, J.-P. (1978). A. Rev. Biochem., 47, 317–57CrossRefGoogle Scholar
  3. Jürss, R. and Maelicke, A. (1980). J. biol. Chem., in pressGoogle Scholar
  4. Jürss, R., Prinz, H. and Maelicke, A. (1979a). Proc. natn. Acad. Sci. U.S.A., 76, 1064–8CrossRefGoogle Scholar
  5. Jürss, R., Prinz, H. and Maelicke, A. (1979b). In Recent Advances in Receptor Chemistry (ed. F. Gualtieri, M. Gianella and C. Melchiorre), Elsevier/North Holland Biomedical Press, Amsterdam, pp. 59–70Google Scholar
  6. Klett, R.P., Fulpius, B.W., Cooper, D., Smith, M., Reich, E. and Possani, L. D. (1973). J. biol. Chem., 248, 6841–53Google Scholar
  7. Lentz, T. L., Mazurkiewicz, J. and Rosenthal, J. (1977). Brain Res., 132, 423–42CrossRefGoogle Scholar
  8. Maelicke, A., Fulpius, B. W., Klett, R. P. and Reich, E. (1977). J. biol. Chem., 252, 4811–30Google Scholar
  9. Mathews-Bellinger, J. and Salpeter, M. M. (1978). J. Physiol., Lond., 279, 197–213CrossRefGoogle Scholar
  10. Nachmansohn, D. (1975). Chemical and Molecular Basis of Nerve Activity, Academic Press, New YorkGoogle Scholar
  11. Neumann, E., Rosenberry, T. L. and Chang, H. W. (1978). In Neuronal Information Transfer (ed. A. Karlin et al.). Academic Press, New York, pp. 183–210Google Scholar
  12. Prinz, H. and Maelicke, A. (1980). To be publishedGoogle Scholar
  13. Rogers, A. W., Darzynkiewicz, Z., Salpeter, M. M., Ostrowski, K. and Barnard, E. A. (1969). J. Cell Biol., 41, 665–85CrossRefGoogle Scholar
  14. Rüchel, R., Watters, D. and Maelicke, A. (1980). To be published.Google Scholar
  15. Salpeter, M. M., Plattner, H. and Rogers, A. W. (1972). Histochem. Cytochem., 20, 1059–68CrossRefGoogle Scholar

Copyright information

© Institute of Biology Endowment Trust Fund 1981

Authors and Affiliations

  • Heino Prinz
    • 1
  • Rolf Jürss
    • 1
  • Alfred Maelicke
    • 1
  1. 1.Max-Planck-InstitutDortmundGermany

Personalised recommendations