Interaction of fluorescent analogues of acetylcholine with nicotinic acetylcholine receptors and acetylcholine esterase

  • Heino Prinz
  • Rolf Jürss
  • Alfred Maelicke
Part of the Biological Council book series


Time-resolved electrophysiological studies of end-plate responses to acetylcholine and cholinergic agonists define the time range for molecular studies in which the events at the neuromuscular junction may be simulated in vitro. Since this time range is of the order of milliseconds, rapid kinetic techniques have to be employed. Suitable ligands for such studies require the following combination of properties: (1) they must have suitable spectroscopic properties, such as fluorescence with high quantum yield, and excitation and emission remote from the ultraviolet-absorption range of proteins; (2) their pharmacological properties must closely resemble those of the natural transmitter and, in particular, must be uncomplicated by side effects which may obscure interpretation of kinetic data.


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Copyright information

© Institute of Biology Endowment Trust Fund 1981

Authors and Affiliations

  • Heino Prinz
    • 1
  • Rolf Jürss
    • 1
  • Alfred Maelicke
    • 1
  1. 1.Max-Planck-InstitutDortmundGermany

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