Abstract
Much has been written concerning the physiological effects of opiates on various species as well as on the localisation of these opiate receptors (for reviews see Reynolds and Randall, 1957; Lewis et al., 1971; Clouet, 1971, Winter, 1975; Kosterlitz et al., 1973). However, with the exception of studies indicating a presynaptic action of opiates which prevents Ca2+-dependent transmitter release and the more recent findings of a postsynaptic action of opiates which controls membrane premeability of Na+ (Zieglgansberger and Bayerl, 1976), little is really known about the biochemical mechanisms underlying opiate receptor function. The discovery that the tissue culture cell line NG108-15 (a hybrid cell made by fusion of a mouse neuroblastoma and a rat glioma cell) contains opiate binding sites which act as regulators of the activity of the cell’s adenylate cyclase affords a model in which to elucidate, in detail, the biochemical bases for one possible opiate receptor function. Although there are a considerable number of similarities between brain and NG108-15 opiate receptors, it should be noted that at present there is not any conclusive evidence that any of the physiological actions of opiates are mediated through regulation of cyclic adenosine monophosphate (cAMP) production.
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© 1981 Institute of Biology Endowment Trust Fund
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Blume, A.J. (1981). NG108-15 opiate receptors: characterization as binding sites and regulators of adenylate cyclase. In: Birdsall, N.J.M. (eds) Drug Receptors and Their Effectors. Biological Council. Palgrave, London. https://doi.org/10.1007/978-1-349-05555-5_4
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DOI: https://doi.org/10.1007/978-1-349-05555-5_4
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