Hepatic Assist 1: Use of Liver Tissue in an Extracorporeal Device

Summary

Hepatic failure and the eventual hepatic coma form a complex pathophysiological state, the aetiology of which remains in most cases undefined and for which treatment has yet to be established. The functions of the liver may be grouped for discussion into those of detoxification and metabolism. Since the function of detoxification is most notable in the early stages of hepatic failure, most attention has been directed to this method of treatment. The rationale for this approach stems from the ability of the liver to regenerate itself and therefore eventually provide total support. However, methods of detoxification alone are limiting and are not sufficient where extended periods of support are necessary, therefore, the need to provide active metabolic activity. For this purpose, liver tissue was chosen. Studies on the procurement and preparation of liver tissue in the form of slices were carried out and the preparation evaluated both in incubation chambers and in specially constructed perfusion devices. Studies have indicated the active function of these preparations for various metabolic processes.