Abstract
Stable isotopes have been used, in conjunction with mass spectrometry, in a variety of biomedical applications. Of these, the most widely exemplified has been the quantification of drugs and their metabolites in biological fluids (e.g. blood, urine), by mass spectrometry—stable-isotope dilution using isotopically labelled analogues as the reference standards. This technique has been extensively reviewed (Jenden and Cho, 1973; Horning et al., 1973; Lawson and Draffan, 1975) and need not be detailed here except to note that, for reasons of cost and ease of synthesis, deuterium has been the most frequently employed isotope, although, in principle, any other stable isotope (e.g. 13C, 15N, 18O, 34S) can be used.
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References
Abdel-Monem, M. M. (1975). J. Med. Chem., 18, 427
Bigeleisen, J. (1949). Science, N.Y. 110, 14
Colvin, M., Padgett, C. A. and Fenselau, C. (1973). Cancer Res., 33, 915
Connors, T. A., Cox, P. J., Farmer, P. B., Foster, A. B. and Jarman, M. (1974a). Biochem. Pharmacol., 23, 115
Connors, T. A., Cox, P. J., Farmer, P. B., Foster, A. B., Jarman, M. and MacLeod, J. K. (1974b). Biomed. Mass Spectrom., 1, 130
Cox, P. J., Farmer, P. B., Foster, A. B., Gilbey, E. D. and Jarman, M. (1976a). Cancer Treatment Rep., 60, 483
Cox, P. J., Farmer, P. B., Jarman, M., Jones, M., Stec, W. J. and Kinas, R. (1976b). Biochem. Pharmacol., 25, 993
Dagne, E., Gruenke, L. and Castagnoli, N. (1974). J. Med. Chem., 17, 1330 Daly, J., Jerina, D. and Witkop, B. (1968). Arch. Biochem. Biophys., 128, 517
Ehrsson, H. (1976). J. Pharm. Pharmacol., 28, 662
Farmer, P. B., Foster, A. B. and Jarman, M. (1975). Biomed. Mass Spectrom., 2, 107
Foster, A. B., Jarman, M., Stevens, J. D., Thomas, P. and Westwood, J. H. (1974). Chem.-Biol. Interactions, 9, 327
Gal, J., Gruenke, L. D. and Castagnoli, N. (1975). J. Med. Chem., 18, 683
Horning, M. G., Nowlin, J., Lertratanangkoon, K., Stillwell, R. N., Stillwell, W. G. and Hill, R. M. (1973). Clin. Chem., 19, 845
Jenden, D. J. and Cho, A. K. (1973). Ann. Rev. Pharmacol., 13, 371
Johnston, T. P., McCaleb, G. S. and Montgomery, J. A. (1975). J. Med. Chem., 18, 634
Kakis, F. J., Fetizon, M., Douchkine, N., Golfier, M., Mourgues, P. and Prange, T. (1974). J. Org. Chem., 39, 523
Kinas, R., Pankiewicz, K. and Stec, W. J. (1975). Bull. Acad. Pol. Sci. Ser. Sci. Chim., 23, 981
Knapp, D. R., Gaffney, T. E. and McMahon, R. E. (1972a). Biochem. Pharmacol., 21, 425
Knapp, D. R., Gaffney, T. E., McMahon, R. E. and Kiplinger, G. (1972b). J. Pharmacol. Exp. Ther., 180, 784
Lawson, A. M. and Draffan, G. H. (1975). Prog. Med. Chem., 12, 1
Lijinsky, W., Loo, J. and Ross, A. E. (1968). Nature, 218, 1174
McGraw, N. P., Caliery, P. S. and Castagnoli, N. (1976). J. Med. Chem. (in press)
May, H. E., Boose, R. and Reed, D. J. (1974). Biochem. Biophys. Res. Commun., 57, 426
Mitoma, C., Yasuda, D. M., Tagg, J. and Tanabe, M. (1967). Biochim. Biophys. Acta, 136, 566
Selander, H. G., Daly, J. and Jerina, D. (1975). Arch. Biochem. Biophys., 168, 309
Struck, R. F., Kirk, M. C., Mellett, L. B., El Dareer, S. and Hill, D. L. (1971). Molec. Pharmacol., 7, 519
Tanaka, N. and Thornton, E. R. (1976). J. Am. Chem. Soc., 98, 1617
Takamizawa, A., Matsumoto, S., Iwata, T., Tochino, Y., Katagiri, K., Yamaguchi, K. and Shiratori, O. (1975). J. Med. Chem., 18, 376
Tomaszewski, J. E., Jerina, D. M. and Daly, J. W. (1975). Biochemistry, 14, 2024.
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Jarman, M., Farmer, P.B., Foster, A.B., Cox, P.J. (1978). The use of deuterium labelling in mechanistic studies of metabolic activation. In: Baillie, T.A. (eds) Stable Isotopes. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-03328-7_7
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DOI: https://doi.org/10.1007/978-1-349-03328-7_7
Publisher Name: Palgrave Macmillan, London
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