The use of deuterium labelling in mechanistic studies of metabolic activation
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Stable isotopes have been used, in conjunction with mass spectrometry, in a variety of biomedical applications. Of these, the most widely exemplified has been the quantification of drugs and their metabolites in biological fluids (e.g. blood, urine), by mass spectrometry—stable-isotope dilution using isotopically labelled analogues as the reference standards. This technique has been extensively reviewed (Jenden and Cho, 1973; Horning et al., 1973; Lawson and Draffan, 1975) and need not be detailed here except to note that, for reasons of cost and ease of synthesis, deuterium has been the most frequently employed isotope, although, in principle, any other stable isotope (e.g. 13C, 15N, 18O, 34S) can be used.
KeywordsStable Isotope Isotope Effect Metabolic Switching Deuterium Labelling Intermediary Metabolite
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- Colvin, M., Padgett, C. A. and Fenselau, C. (1973). Cancer Res., 33, 915Google Scholar
- Cox, P. J., Farmer, P. B., Foster, A. B., Gilbey, E. D. and Jarman, M. (1976a). Cancer Treatment Rep., 60, 483Google Scholar
- Dagne, E., Gruenke, L. and Castagnoli, N. (1974). J. Med. Chem., 17, 1330 Daly, J., Jerina, D. and Witkop, B. (1968). Arch. Biochem. Biophys., 128, 517Google Scholar
- Horning, M. G., Nowlin, J., Lertratanangkoon, K., Stillwell, R. N., Stillwell, W. G. and Hill, R. M. (1973). Clin. Chem., 19, 845Google Scholar
- Kinas, R., Pankiewicz, K. and Stec, W. J. (1975). Bull. Acad. Pol. Sci. Ser. Sci. Chim., 23, 981Google Scholar
- Knapp, D. R., Gaffney, T. E., McMahon, R. E. and Kiplinger, G. (1972b). J. Pharmacol. Exp. Ther., 180, 784Google Scholar
- McGraw, N. P., Caliery, P. S. and Castagnoli, N. (1976). J. Med. Chem. (in press)Google Scholar
- Struck, R. F., Kirk, M. C., Mellett, L. B., El Dareer, S. and Hill, D. L. (1971). Molec. Pharmacol., 7, 519Google Scholar