Advertisement

Mass spectrometric methods of isotope determination

  • J. Throck Watson

Abstract

Although there are several analytical techniques for determining the isotopic composition of a sample, combined gas chromatography—mass spectrometry (GC—MS) is often the most suitable for analysis of samples of biological origin (Watson, 1976). For example, biological samples are rarely pure (even after extensive sample processing) and the biologically active component may represent only a trace constituent of the total sample. The combination of a separation technique with an identification technique in GC—MS permits a complex mixture to be separated in the time domain so that characteristic mass spectra can be obtained from picomole to nanomole quantities of sample depending on the mode of instrument operation.

Keywords

Mass Spectrometric Method Urine Extract Biological Extract Discernible Peak Expect Retention Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Änggård, E. and Sedvall, G. (1969). Anal. Chem., 41, 1250CrossRefGoogle Scholar
  2. Axen, U., Gréen, K., Horlin, D. and Samuelsson, B. (1971). Biochem. Biophys. Res. Commun., 45, 519CrossRefGoogle Scholar
  3. Biller, J. W. and Biemann, K. (1974). Anal. Lett., 7, 515CrossRefGoogle Scholar
  4. Braselton, W. E., Orr, J. C. and Engel, L. L. (1973). Anal. Biochem., 53, 64CrossRefGoogle Scholar
  5. Bush, M. T., Sekerke, H. J. Jr., Vore, M., Sweetman, B. J. and Watson, J. T. (1972). Proceedings of Seminar on the Use of Stable Isotopes in Clinical Pharmacology (ed. P. D. Klein and L. J. Roth), Technical Information Center, CONF-711115, Springfield, Va., USA, p. 233Google Scholar
  6. Falkner, F. C., Sweetman, B. J. and Watson, J. T. (1975). Appl. Spectrosc. Rev., 10, 51CrossRefGoogle Scholar
  7. Farmer, J. B. (1963). Mass Spectrometry (ed. C. A. McDowell), McGraw-Hill, New York, Chapter 2, p. 7Google Scholar
  8. Frölich, J. C., Wilson, T. W., Sweetman, B. J., Nies, A. S., Carr, K., Watson, J. T. and Oates, J. A. (1975). J. Clin. Invest., 55, 763CrossRefGoogle Scholar
  9. Gréen, K. (1969). Chem. Phys. Lipids, 3, 254CrossRefGoogle Scholar
  10. Hammar, C.-G., Alexanderson, B., Holmstedt, B. and Sjöqvist, F. (1971). Clin. Pharmacol. Therap., 12, 496Google Scholar
  11. Hammar, C.-G., Holmstedt, B. and Ryhage, R. (1968). Anal. Biochem., 25, 532CrossRefGoogle Scholar
  12. Heath, E. C., Falkner, F. C., Hill, R. E. and Watson, J. T. (1974). Proceedings of the 22nd Annual Conference on Mass Spectrometry and Allied Topics, Philadelphia, American Society for Mass Spectrometry, p. 286Google Scholar
  13. Hites, R. A. and Biemann, K. (1967). Anal. Chem., 39, 965CrossRefGoogle Scholar
  14. Hites, R. A. and Biemann, K. (1970). Anal. Chem., 42, 855CrossRefGoogle Scholar
  15. Hoffenberg, R., Lawson, A. M., Ramsden, D. R. and Raw, P. J. (1973). Mass Spectrometry in Biochemistry and Medicine (ed. A. Frigerio and N. Castagnoli), Raven Press, New York, p. 303Google Scholar
  16. Holmes, W. F., Holland, W. H. and Parker, J. A. (1971). Anal. Chem., 43, 1806CrossRefGoogle Scholar
  17. Holmstedt, B. and Lindgren, J.-E. (1974). The Poisoned Patient: The Role of the Laboratory, Ciba Foundation Symposium 26, ASP (Elsevier—Exerpta Medica—North-Holland), Amsterdam, p. 106Google Scholar
  18. Holmstedt, B. and Palmér, L. (1973). Advan. Biochem. Psychopharmacol., 7, 1Google Scholar
  19. Knapp, D. R., Gaffney, T. W. and McMahon, R. E. (1972). Biochem. Pharmacol., 21, 425CrossRefGoogle Scholar
  20. Pohl, L. R., Nelson, S. D., Garland, W. A. and Trager, W. F. (1975). Biomed. Mass Spectrom., 2, 23CrossRefGoogle Scholar
  21. Samuelsson, B., Hamberg, M. and Sweeley, C. C. (1970). Anal. Biochem., 33, 301CrossRefGoogle Scholar
  22. Sweeley, C. C., Elliott, W. H., Fries, I. and Ryhage, R. (1966). Anal. Chem., 38, 1549CrossRefGoogle Scholar
  23. Sweeley, C. C., Ray, B. D., Wood, W. K., Holland, J. F. and Krichevsky, M. I. (1970). Anal. Chem., 42, 1505CrossRefGoogle Scholar
  24. Vore, M., Sweetman, B. J. and Bush, M. T. (1974). J. Pharmacol. Exp. Ther., 190, 384Google Scholar
  25. Waller, G. R. (1972). Biochemical Applications of Mass Spectrometry, Wiley-Interscience, New YorkGoogle Scholar
  26. Watson, J. T. (1976). Introduction to Mass Spectrometry for Biomedical, Environmental and Forensic Application, Raven Press, New YorkGoogle Scholar
  27. Watson, J. T., Falkner, F. C. and Sweetman, B. J. (1974). Biomed. Mass Spectrom., 1, 156CrossRefGoogle Scholar
  28. Watson, J. T., Pelster, D. R., Sweetman, B. J., Frölich, J. C. and Oates, J. A. (1973). Anal. Chem., 45, 2071CrossRefGoogle Scholar

Copyright information

© The Contributors 1978

Authors and Affiliations

  • J. Throck Watson
    • 1
  1. 1.Department of PharmacologyVanderbilt UniversityNashvilleUSA

Personalised recommendations