Mass spectrometric methods of isotope determination

  • J. Throck Watson


Although there are several analytical techniques for determining the isotopic composition of a sample, combined gas chromatography—mass spectrometry (GC—MS) is often the most suitable for analysis of samples of biological origin (Watson, 1976). For example, biological samples are rarely pure (even after extensive sample processing) and the biologically active component may represent only a trace constituent of the total sample. The combination of a separation technique with an identification technique in GC—MS permits a complex mixture to be separated in the time domain so that characteristic mass spectra can be obtained from picomole to nanomole quantities of sample depending on the mode of instrument operation.


Mass Spectrometric Method Urine Extract Biological Extract Discernible Peak Expect Retention Time 
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© The Contributors 1978

Authors and Affiliations

  • J. Throck Watson
    • 1
  1. 1.Department of PharmacologyVanderbilt UniversityNashvilleUSA

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