Application and potential of coenzyme labelling

  • T. Cronholm
  • T. Curstedt
  • J. Sjövall


Reaction rates and equilibria in intermediary metabolism are regulated by several factors such as substrate concentrations, allosteric effects and feed-back control of enzyme synthesis. Another factor that may be of importance is the interaction between reactions that utilise the same coenzyme. A typical example is the decreased conversion of lactate to pyruvate during ethanol oxidation, mediated by the increased ratio between the concentrations of free (i.e. not protein-bound) NADH and NAD+ in the liver cells. Compartmentation of redox reactions and coenzymes may determine the degree of interaction. For example, the impermeability of the mitochondrial membrane to pyridine nucleotides results in the well-known existence of cytosolic and mitochondrial pools of NAD with widely different redox states. The diffusion rate in the cytosol is generally considered to be sufficiently high to prevent the formation of several coenzyme pools in this compartment. However, the equilibration of NAD bound to enzymes may be limited by slow dissociation rates. Another possibility for compartmentation of coenzymes when whole organs are studied is the existence of functionally different cell types in the organ.


Bile Acid Stable Isotope Ethanol Oxidation Pyridine Nucleotide Glycerol Moiety 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Carcinero, H. H., Moore, C. L. and Hoberman, H. D. (1972). J. Biol. Chem., 247, 418Google Scholar
  2. Cronholm, T. (1972). Europ. J. Biochem., 27, 10CrossRefGoogle Scholar
  3. Cronholm, T. (1974). Europ. J. Biochem., 43, 189CrossRefGoogle Scholar
  4. Cronholm, T., Burlingame, A. L. and Sjövall, J. (1974a). Europ. J. Biochem., 49, 497CrossRefGoogle Scholar
  5. Cronholm, T. and Curstedt, T. (1977). In preparationGoogle Scholar
  6. Cronholm, T. and Fors, C. (1976). Europ. J. Biochem., 70, 83CrossRefGoogle Scholar
  7. Cronholm, T., Makino, I. and Sjövall, J. (1972a). Europ. J. Biochem., 24, 507CrossRefGoogle Scholar
  8. Cronholm, T., Makino, I. and Sj6vall, J. (1972b). Europ. J. Biochem., 26, 251CrossRefGoogle Scholar
  9. Cronholm, T., Matern, H., Matern, S. and Sjövall, J. (1974b). Europ. J. Biochem., 48, 71CrossRefGoogle Scholar
  10. Cronholm, T. and Sjövall, J. (1970). Europ. J. Biochem., 13, 124CrossRefGoogle Scholar
  11. Curstedt, T. (1974a). Biochem. Biophys. Acta, 369, 196CrossRefGoogle Scholar
  12. Curstedt, T. (1974b). Eur. J. Biochem., 49, 355CrossRefGoogle Scholar
  13. Curstedt, T. and Sjövall, J. (1974). Biochem. Biophys. Acta, 369, 173CrossRefGoogle Scholar
  14. Hassinen, I. (1967). Ann. Med. Exp. Fenn., 45, 35Google Scholar
  15. Hoberman, H. D. (1958). J. Biol. Chem., 232, 9Google Scholar
  16. Hung, H. C. and Hoberman, H. D. (1972). Biochem. Biophys. Res. Commun., 46, 399CrossRefGoogle Scholar
  17. Jungas, R. L. (1968). Biochemistry, 7, 3708CrossRefGoogle Scholar
  18. Mützel, W. and Wenzel, M. (1971). Hoppe-Seyler’s Z. Physiol. Chem., 352, 304CrossRefGoogle Scholar
  19. Nabors, C. J., Berliner, D. L. and Dougherty, T. F. (1967). J. Reticuloend. Soc., 4, 237Google Scholar
  20. Pollow, K., Sokolowski, G., Schmalbeck, J. and Pollow, B. (1974). Hoppe-Seyler’s Z. Physiol. Chem., 355, 515CrossRefGoogle Scholar
  21. Rognstad, R. and Clark, D. G. (1974). Europ. J. Biochem., 42, 51CrossRefGoogle Scholar
  22. Rognstad, R. and Katz, J. (1970). Biochem. J., 116, 483CrossRefGoogle Scholar
  23. Rous, S. (1971). Adv. Lipid Res., 9, 73Google Scholar
  24. Threlfall, C. J. and Heath, D. F. (1968). Biochem. J., 110, 303CrossRefGoogle Scholar
  25. Wadke, M., Brunengraber, H., Lowenstein, J. M., Dolhun, J. J. and Arsenault, G. P. (1973). Biochemistry, 12, 2619CrossRefGoogle Scholar
  26. Wenzel, M., Bollert, B. and Ahlers, B. (1974). Hoppe-Seyler’s Z. Physiol. Chem., 355, 969CrossRefGoogle Scholar
  27. Wenzel, M., Pitzel, L. and Riesselmann, B. (1975). Hoppe-Seyler’s Z. Physiol Chem., 356, 459CrossRefGoogle Scholar
  28. Zehner, J., Loy, E., Müllhofer, G. and Bücher, T. (1973). Euro p. J. Biochem., 34, 248CrossRefGoogle Scholar

Copyright information

© The Contributors 1978

Authors and Affiliations

  • T. Cronholm
    • 1
  • T. Curstedt
    • 1
  • J. Sjövall
    • 1
  1. 1.Department of ChemistryKarolinska InstitutetStockholmSweden

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