Substrate and inhibitor binding to dihydrofolate reductase
Part of the Biological Council book series (BCSDA)
Dihydrofolate reductase (5, 6, 7, 8-tetrahydrofolate:NADP+ oxidoreductase, E.C.126.96.36.199), an enzyme found in the vast majority of both prokaryotic and eukaryotic organisms, catalyses the reduction of dihydrofolate to tetrahydrofolate, using NADPH as coenzyme:
The product of this reaction, tetrahydrofolate, plays a vital role in intermediary metabolism, acting as a ‘carrier’ of one-carbon fragments in the biosynthesis of a number of amino acids, thymidylate and purines (for a review, see Blakley, 1969).
KeywordsTyrosine Residue Folinic Acid Histidine Residue Nuclear Magnetic Reson Spectroscopy Dihydrofolate Reductase
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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- Baker, B. R. (1959). Cancer Chemother. Rep., 4, 1Google Scholar
- Baker, B. R. (1967). Design of Active -site -directed Irreversible Enzyme Inhibitors, New York, WileyGoogle Scholar
- Bertino, J. R. (Ed.) (1971). Folate antagonists as chemotherapeutic agents. Ann. N. Y. Acad. Sci., 186Google Scholar
- Birdsall, B., Feeney, J., Roberts, G. C. K. and Burgen, A. S. V. (1977a). Manuscript in preparationGoogle Scholar
- Birdsall, B., Griffiths, D. V., Roberts, G. C. K., Feeney, J. and Burgen, A. S. V. (1977b). Proc. R. Soc. London, in press.Google Scholar
- Blakley, R. L. (1969). The Biochemistry of Folic Acid and Related Pteridines. Amsterdam, North -HollandGoogle Scholar
- Feeney, J., Roberts, G. C. K., Birdsall, B., Griffiths, D. V., King, R. W., Scudder, P. and Burgen, A. S. V. (1977). Proc. R. Soc. London, in pressGoogle Scholar
- Mihich, E. (Ed.) (1973). Drug Resistance and Sensitivity: Biochemical and Cellular Basis, New York, Academic PressGoogle Scholar
- Poe, M., Bennett, C. D., Donoghue, D., Hirshfield, J. M., Williams, M. N. and Hoogsteen, K. (1975). In Chemistry and Biology of Pteridines (Proc. 5th Int. Symp.) (ed. W. Pfleiderer ), p. 51Google Scholar
- Poe, M., Greenfield, N. J., Hirshfield, J. M. and Hoogsteen, K. (1974). Cancer Biochem. Biophys., 1, 7Google Scholar
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