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Preparation of biospecific supports for affinity chromatography and immunoadsorption

  • S. Comoglio
  • A. Massaglia
  • E. Rolleri
  • U. Rosa

Abstract

In recent years much attention has been devoted to the techniques of affinity chromatography for studying complex biological systems1. The basic principle is to immobilise one of the components of the interacting system by binding to an insoluble support, preferably through covalent bonds2,3. The biologically active support can be used to separate selectively from the reaction mixture the component with which it interacts. A successive elution yields pure components4–6. This technique consequently looks very promising both as an analytical method7 and for studying the mode of action of biological substances8,9.

Keywords

Affinity Chromatography Coupling Reaction Coupling Yield Active Ester Scatchard Plot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Cuatrecasas, P. and Anfinsen, C. B. Affinity chromatography. Ann. Rev. Biochem., 40 (1971), 259CrossRefGoogle Scholar
  2. 2.
    Silman, I. H. and Katchalski, E. Water-insoluble derivatives of enzymes, antigens, and antibodies. Ann. Rev. Biochem., 35 (1966), 873CrossRefGoogle Scholar
  3. 3.
    Cuatrecasas, P. Protein purification by affinity chromatography. J. Biol. Chem., 245 (1970), 3059Google Scholar
  4. 4.
    Sato, N. and Carcille, M. Separation of specific from nonspecific anti-FSH antibody by affinity chromatography on Sepharose-HCG. Endocrinology, 90 (1972), 302CrossRefGoogle Scholar
  5. 5.
    Pensky, J. and Marshall, J. S. Studies on thyroxine-binding globulin (TBG). Arch. Biochem. Biophys., 135 (1969), 304CrossRefGoogle Scholar
  6. 6.
    Steers, E. Jr., Cuatrecasas, P. and Pollard, H. B. The purification of β-galactosidase from Escherichia Coli by affinity chromatography. J. Biol. Chem., 246 (1971), 196Google Scholar
  7. 7.
    Weintraub, B. D. and Kadesky, Y. M. Fractionation of antibodies to human chorionic somatomammotropin: application to radioimmunoassay. J. Clin. Endocr., 33 (1971), 432CrossRefGoogle Scholar
  8. 8.
    Rosenberry, T. L., Chang, H. W. and Chen, Y. T. Purification of acetylcholinesterase by affinity chromatography and determination of active site stoichiometry. J. Biol. Chem., 247 (1972), 1555Google Scholar
  9. 9.
    Olesen, H., Molin, J., Hippe, E., Rye, M., Thomsen, J., Lee, L. and Haber, E. (1972). Studies on human intrinsic factor purified by affinity chromatography. 8th International Congress on Clinical Chemistry. Copenhagen, 18–23 June, abs. in Scand. J. Clin. Lab. Invest., 29, sup. 126 (1972).Google Scholar
  10. 10.
    Wide, L. Radioimmunoassay employing immunosorbents. Acta Endocrin., Copenhagen, 63, suppl. 142 (1969), 207Google Scholar
  11. 11.
    Cuatrecasas, P. and Parikh, I. Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose. Biochem., 11 (1972), 2291CrossRefGoogle Scholar
  12. 12.
    Ludens, J. H., De Vries, J. R. and Fanestil, D. D. Criteria for affinity chromatography of steroid-binding macromolecules. J. Biol. Chem., 247 (1972), 7533Google Scholar
  13. 13.
    Massaglia, A., Rolleri, E., Barbieri, U. and Rosa, U. Fractionation of antibodies to testosterone. J. Clin. Endocrinol. Metab., 38 (1974), 820CrossRefGoogle Scholar
  14. 14.
    Sica, V., Parikh, I., Nola, E., Puca, G. A. and Cuatrecasas, P. Affinity chromatography and the purification of oestrogen receptors. J. Biol. Chem., 248 (1973), 6543Google Scholar
  15. 15.
    Rosner, W. and Bradlow, H. L. Purification of corticosteroid-binding globulin from human plasma by affinity chromatography. J. Clin. Endocrinol. Metab., 33 (1971), 193CrossRefGoogle Scholar
  16. 16.
    Burstein, S. H. The removal of testosterone binding globulin from plasma by affinity chromatography. Steroids., 14 (1969), 263CrossRefGoogle Scholar
  17. 17.
    Ludens, J. H., De Vries, J. R. and Fanestil, D. D. Studies on affinity chromatography of aldosterone-binding macromolecules. Steroid Biochem., 3 (1972), 192CrossRefGoogle Scholar
  18. 18.
    Vonderhaar, B. and Muller, G. C. Binding of oestrogen receptor to oestradiol immobilised on insoluble resins. Biochem. Biophys. Acta, 176 (1969), 626CrossRefGoogle Scholar
  19. 1.
    Bolton, A. E. and Hunter, W. M. The use of antisera covalently coupled to agarose, cellulose and Sephadex in radioimmunoassay systems for proteins and haptens. Biochim. Biophys. Acta, 329 (1973), 318CrossRefGoogle Scholar
  20. 2.
    Travis, J. and Pannel, R. Selective removal of albumin from plasma by affinity chromatography. Clinica Chimica Acta, 49 (1973), 49CrossRefGoogle Scholar

Copyright information

© The Contributors 1976

Authors and Affiliations

  • S. Comoglio
  • A. Massaglia
  • E. Rolleri
  • U. Rosa

There are no affiliations available

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