Preparation of biospecific supports for affinity chromatography and immunoadsorption
In recent years much attention has been devoted to the techniques of affinity chromatography for studying complex biological systems1. The basic principle is to immobilise one of the components of the interacting system by binding to an insoluble support, preferably through covalent bonds2,3. The biologically active support can be used to separate selectively from the reaction mixture the component with which it interacts. A successive elution yields pure components4–6. This technique consequently looks very promising both as an analytical method7 and for studying the mode of action of biological substances8,9.
KeywordsAffinity Chromatography Coupling Reaction Coupling Yield Active Ester Scatchard Plot
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