Abstract
The early progress in biochemical studies of putative neurotransmitters in the central nervous system (CNS) was fostered by bioassay (Gaddum, 1959) and fluorometric procedures (Von Euler, 1959; Weil-Malherbe, 1959; Bogdanski, Pletscher, Brodie and Udenfriend, 1956) to measure acetylcholine (ACh), noradrenaline (NA), dopamine (DA) and serotonin (5-hydroxytryptamine, 5-HT) concentrations. The sensitivity of bioassay procedures was always greater than that of fluorescence analysis. The latter, however, offered some direct information on the chemical nature of the compound under analysis. There is accumulating evidence that amino acids, which are closely related structurally, may function in synaptic transmission, and it has become necessary to resort to methods of high sensitivity and specificity to measure transmitters at synaptic level.
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References
Bertilsson, L., Atkinson. A. J., Althaus, J. J. R., Marfast, A., Lindgren, J. F. and Holmstedt, B. (1972). ‘Quantitative determination of 5-hydroxyindole-3-acetic acid in cerebrospinal fluid by gas chromatography-mass spectrometry’, Analyt. Chem., 44, 1434–1438
Bogdanski, D. F., Pletscher, A., Brodie, B. B. and Udenfriend, S. (1956). ‘Identification and assay of serotonin in brain’, J. Pharmac. exp. Ther., 117, 82–88
Brodie, B. B., Costa, E., Dlabac, A., Neff, N. H. and Smookler, M. M. (1966). ‘Application of steady-state kinetics to the estimation of synthesis rate and turnover time of tissue catecholamines’, J. Pharmac. exp. Ther., 154, 493–498
Brownstein, M. J., Saavedra, J. M., Palkovits, M. and Axelrod, J. (1974). ‘Histamine content of hypothalamic nuclei of the rat’, Brain Res. 77, 151–156
Bunney, B. S., Walters, J. R., Roth, R. H. and Aghajanian, G. K. (1973). Dopaminergic neurons: effect of antipsychotic drugs and amphetamine on single cell activity’, J. Pharmac. exp. Ther., 185, 570–588
Cattabeni, F., Koslow, S. H. and Costa, E. (1972). ‘Gas chromatography—mass spectrometry assay of four indolealkylamines of the rat pineal’, Science, 178, 166–168
Clement-Cormier, Y. C., Kebabian, J. W., Petzold, G. L. and Greengard, P. (1974). ‘Dopamine sensitive adenylate cyclase in mammalian brain: a possible site of action of antipsychotic drugs’, Proc. natn. Acad. Sci. U.S.A., 71, 1113–1117
Costa, E., Bjegovic, M. and Koslow, S. H. (1974). ‘Mass fragmentography: a method to study monoamine neurotransmitters in brain nuclei’, in Psychoneuroendocrinology. (Hatotani, N. and Mieken, Tsu, Eds.) 304–312, Karger, Basel
Costa, E., Green, A. R., Koslow, S. H., LeFevre, H. F., Revuelta, A. V. and Wang, C. (1972). ‘Dopamine and norepinephrine in noradrenergic axons: a study in vivo of their precusor product relationship by mass fragmentography and radiochemistry’, Pharmac. Rev., 24, 167–189
Costa, E., Koslow, S. H. and LeFevre, H. F. (1975). ‘Mass fragmentography: a tool for studying transmitter function at synaptic level’, in Handbook of Psychopharmacology, (Iversen, L. L., Iversen, S. D. and Snyder, S. H., Eds.) 8–24, Plenum, New York
Costa, E. and Neff, N. H. (1970). ‘Estimation of turnover rates to study the metabolic regulation of the steady-state level of neuronal monoamines’, in Handbook of Neurochemistry Vol. 4 (Lajtha, A. Ed.) 45–90, Plenum, New York
Costa, E. and Trabucchi, M. (1974). ‘Regulation of brain dopamine turnover rate: pharmacological implications’, in Catecholamines and Behaviour (Friedhoff, A.J., Ed.) Plenum, New York
Coyle, J. T. and Henry, D. (1973). ‘Catecholamines in fetal and newborn rat brain’, J. Neurochem., 21, 61–67
Doteuchi, M., Wang, C. and Costa, E. (1973). ‘Compartmentation of dopamine in rat striatum’, Molec. Pharmac., 10, 225–234
Fonnum, F., Gropova, I., Rinvik, E., Storm-Mathisen, J. and Walberg, F. (1974). ‘Origin and distribution of glutamate decarboxylase in substantia nigra of the cat’, Brain Res., 71, 77–82
Fuxe, K., Hökfelt, T., Jonsson, G. and Ungerstedt, U. (1970). ‘Fluorescence microscopy in neuroanatomy’, in Contemporary Research Methods in Neuroanatomy (Nauta, W. J. H. and Ebbesson, S. O. E., Eds.) 275–314, Springer Verlag, Berlin
Fuxe, K., Hökfelt, T. and Ungerstedt, U. (1970). ‘Morphological and functional aspects of central monoamine neurons’, Int. Rev. Neurobiol., 13, 93–126
Fuxe, K. and Ungerstedt, U. (1970). ‘Histochemical, biochemical and functional studies on central monoamine neurons after acute and chronic amphetamine administration’, in Amphetamines and Related Compounds (Costa, E. and S., Garattini, Eds.) 257–288, Raven Press, New York
Gaddum, J. H. (1959). ‘Bioassay procedures’, Pharmac. Rev., 11, 278–288
Goldberg, A. M. and McAaman (1973). ‘The determination of picomole amounts of acetylcholine in mammalian brain’, J. Neurochem., 20, 1–8
Guidotti, A., Cheney, D. L., Trabucchi, M., Doteuchi, M., Wang, C. and Hawkins, R. A. (1974). ‘Focused microwave radiation: a technique to minimize postmortem changes of cyclic nucleotides, DOPA and choline and to preserve brain morphology’, Neuropharmacology, 13, 1115–1122
Hanin, I. and Schuberth, J. (1974). ‘Labelling of acetylcholine in the brain of mice fed on a diet containing deuterium labeled choline: studies utilizing gas chromatography-mass spectrometry’, J. Neurochem., 23, 819–824
Jacobowitz, D. M. and Palkovits, M. (1974). ‘Topographic atlas of catecholamine and acetylcholinesterase-containing neurons in the rat brain. I. Forebrain (telencephalon, diencephalon)’, J. comp. Neurol., 157, 13–28
Koslow, S. H., Cattabeni, F. and Costa, E. (1972). ‘Norepinephrine and dopamine: assay by mass fragmentography in the picomole range’, Science, 176, 177–180
Koslow, S. H. and Green, A. R. (1973). ‘Analysis of pineal and brain indolealkylamines by gas chromatography-mass spectrometry’, in Advances in Biochemical Psychopharmacology, Vol. 7, (Costa, E. and Holmstedt, B., Eds.) 33–43 Raven Press, New York
Koslow, S. H., Racagni, G. and Costa, E. (1974). ‘Mass fragmentography measurements of norepinephrine, dopamine, serotonin and acetylcholine in seven discrete nuclei of the rat tel-diencephaIon’, Neuropharmacology, 13, 1123–1130
Koslow, S. H. and Schlumpf,.M. (1974). ‘Quantitation of epinephrine in rat brain nuclei and areas by mass fragmentography’, Nature, 251, 530–531
Lewis, P. R. and Shute, C. C. D. (1967). ‘The cholinergic limbic system: projections to hippocampal formation, medial cortex, nuclei of the ascending cholinergic reticular system and the subformical organ and supraoptic crest’, Brain, 90, 521–542
Miller, R. J. and Hiley, C. R. (1974). ‘Antimuscarinic properties of neuroleptics and drug induced parkinsonism’, Nature, 248, 596–602
Palkovits, M., Brownstein, M., Saavedra, J. M. and Axelrod, J. (1974). ‘Norepinephrine and dopamine content of hypothalamic nuclei of the rat’, Brain Res., 77, 137–149
Palkovits, M. and Jacobowitz, D. M. (1974). ‘Topographic atlas of catecholamine and acetylcholinesterase-containing neurons in the rat brain. 11. Hindbrain (mesencephalon, rhombencephalon)’, J. comp. Neurol., 157, 29–42
Saavedra, J. M., Brownstein, M. and Axelord, J. (1973). ‘A specific and sensitive microassay for serotonin in tissues’, J. Pharmac. exp. Ther., 186, 508–515
Saavedra, J. M., Palkovits, M., Brownstein, M. J. and Axelord, J. (1974). ‘Serotonin distribution in the nuclei of the rat hypothalamus and preoptic region’, Brain Res., 77, 157–165
Shute, C. C. D. and Lewis, P. R. (1967). ‘The ascending cholinergic reticular system: neocortical, olfactory and subcortical projections’, Brain, 90, 497–520
Silver, A. (1967). ‘Cholinesterase of the central nervous system with special reference to the cerebellum’, Int. Rev..Neurobiol., 10, 57–109
Sjoqvist, B., Dailey, J., Sedvall, G. and Anggard, E. (1973). ‘Mass fragmentographic assay of homovanillic acid in brain tissue’, J. Neurochem., 20, 729–733
Trabucchi, M., Cheney, D., Racagni, G. and Costa, E. (1974) ‘Involvement of brain cholinergic mechanisms in the action of chlorpromazine’, Nature, 249, 664–666
Trabucchi, M., Cheney, D. L., Racagni, G. and Costa, E. (1975). In vivo inhibition of striatal acetylcholine turnover by L-DOPA, apomorphine and ( + )-amphetamine’, Brain Res., 85, 130–134
Von Euler, U. S. (1959). The development and application of the trihydroxyindole method for catecholamines’, Pharmac. Rev., 11, 262–268
Waldron, H. A. and Gwyn, D. G. (1969). ‘Acetylcholinesterase activity in the red nucleus of the rat and its response to axotomy’, Brain Res., 13, 146–154
Weil-Malherbe, H. (1959). ‘The fluorometric estimation of catechol compounds by the ethylenediamine condensation method’, Pharmac. Rev., 11, 278–288
Zivkovic, B., Guidotti, A., Revuelta, A. and Costa, E. (1975). ‘Effect of thioridazine, clozapine and other antipsychotics on the kinetic state of tyrosine hydroxylase and on the turnover rate of dopamine in striatum and nucleus accumbens’, J. Pharmac. exp. Ther. 194, 37–46
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Costa, E., Cheney, D.L., Racagni, G. (1976). Steady state and dynamics of putative neurotransmitters in tel-diencephalic nuclei: a study using multiple ion detection. In: Bradley, P.B., Dhawan, B.N. (eds) Drugs and Central Synaptic Transmission. Palgrave, London. https://doi.org/10.1007/978-1-349-01247-3_3
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DOI: https://doi.org/10.1007/978-1-349-01247-3_3
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