Aequorin-Calcium luminescence and Its Application to Muscle Physiology

  • C. C. Ashley
  • E. B. Ridgway
Part of the Biological Council book series


There is much evidence to suggest that small transient changes in intracellular calcium play an important intermediate role in the complex process of excitation-contraction (E-C) coupling in skeletal muscle (Weber, Herz & Reiss, 1964; Portzehl, Caldwell & Rüegg, 1964; Jöbsis & O’Connor, 1966; Hellam & Podolsky, 1969). These changes in intracellular calcium can now be monitored during the contraction of a single muscle fibre by a photometric method (Ridgway & Ashley, 1967; Ashley & Ridgway, 1968, 1969, in preparation; Ashley, 1969). The method uses the remarkable properties of the photoprotein, aequorin, which is extracted from the photogenic organs of the hydromedusa, Aequorea forskalea.


Hypertonic Saline Calcium Transient Tension Response Isometric Tension Potassium Citrate 
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Copyright information

© Palgrave Macmillan, a division of Macmillan Publishers Limited 1970

Authors and Affiliations

  • C. C. Ashley
    • 1
  • E. B. Ridgway
    • 2
  1. 1.Department of ZoologyUniversity of BristolUK
  2. 2.Department of BiologyUniversity of OregonUK

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