Calcium Chelation and Buffers
An approach which has proved very useful in studies of the role of calcium in biological systems, in particular in muscle, is the use of substances which selectively bind this ion. One of the best known compounds of this type is ethylene-diamine-tetra-acetic acid (EDTA) which binds calcium about 100 times more effectively than it binds magnesium. In recent years, however, EDTA has been superseded in many studies involving calcium by ethylene-glycol bis (β-amino-ethyl-ether)-N,N′-tetra-acetic acid (EGTA) which can bind calcium over 105 times more effectively than it binds magnesium. This article is concerned with some of the uses which have been made of EGTA in studying the role of calcium in the contraction of the large single muscle fibres which can be obtained from certain crustacea, in particular crabs (Maia squinado) and barnacles (Balanus nubilus).
KeywordsAssociation Constant Contractile Response Ionize Calcium Concentration Calcium Chelation Single Muscle Fibre
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